Search results for "test"

Generation and neutralization of pseudovirions of human papillomavirus type 33

1997

Since human papillomaviruses (HPV) cannot be propagated in cell culture, the generation of infectious virions in vitro is a highly desirable goal. Here we report that pseudovirions can be generated by the assembly of virus-like particles (VLPs) in COS-7 cells containing multiple copies of a marker plasmid. Using recombinant vaccinia viruses, we have obtained spherical VLPs of HPV type 33 (HPV-33) which fractionate into heavy and light VLPs in cesium chloride density gradients. VLPs in the heavy fraction (1.31 g/cm3) carry the plasmid in DNase-resistant form and are capable of transferring the genetic marker located on the plasmid to COS-7 cells in a DNase-resistant way (pseudoinfection). Th…

Rapid and sensitive detection of metapneumovirus in clinical specimens by indirect fluorescence assay using a monoclonal antibody.

2008

Human metapneumovirus, with two known genotypes named A and B, is associated with mild respiratory symptoms to severe LRTI in children, high-risk adults and the elderly. Rapid and reliable methods of hMPV detection in clinical samples are essential to implement appropriate care, to better understand the pathology of hMPV and to determine its epidemiology. Respiratory samples from 1,386 patients collected during 2 consecutive years were screened for hMPV using indirect immunofluorescence (IFA) assay with a monoclonal antibody. Forty-three patients tested positive for hMPV by the IFA method. In parallel, the samples were examined with RT-PCR on the F gene. Of these, 41 specimens were RT-PCR p…

Genome sequence of SeIV-1, a novel virus from the Iflaviridae family infective to Spodoptera exigua

2011

Analysis of the transcriptome of Spodoptera exigua larvae revealed the presence of several ESTs with homology to virus of the order Picornavirales and with the highest similarity to Infectious flacherie virus (Iflaviridae) that infects Bombyx mori larvae. Iflaviridae is a recently defined family of insect-infecting viruses that consist of positive single strand RNA genomes translated into a single polyprotein of around 3000 amino acids long. Using the sequence information derived from the obtained ESTs, we have completed the genomic sequence of this virus. The novel S. exigua iflavirus (SeIV-1) has a genome of 10.3 kb and codes for a 3222 aa polyprotein. Expression analysis has revealed the…

Esercizio fisico ed emoreologia

2010

Development and characterisation of polymer composite materials with bio-inspired functions

2018

Promocijas darbs ir izstrādāts disertācijas veidā fizikas nozarē materiālu fizikas apakšnozarē. Pētījums tika veikts LU Materiālu mehānikas institūtā. Darbs saistīts ar aktuālu un jaunu pētījumu virzienu: bioinspirēto funkciju tālāku attīstīšanu slodzi nesošos kompozītos. Tās ir mehānisku triecienu vizuālās indikācijas un polimēru matricas vaskulārās pašdziedināšanas funkcijas. Šīs funkcijas tika nodrošinātas, izveidojot viedus slāņus un iebūvējot tos kompozītā. Pētījumā tika noskaidrotas piemērotās pārbaužu secības, attīstītas kvantitatīvas metodes un mehānisks modelis, lai novērtētu jauno funkciju efektivitāti kompozītos. Ir pierādīta funkciju augstā efektivitāte un spēja uzlabot slodzi n…

Explicit behavioral detection of visual changes develops without their implicit neurophysiological detectability

2011

Change blindness is a failure of reporting major changes across consecutive images if separated, e.g., by a brief blank interval. Successful change detection across interrupts requires focal attention to the changes. However, findings of implicit detection of visual changes during change blindness have raised the question of whether the implicit mode is necessary for development of the explicit mode. To this end, we recorded the visual mismatch negativity (vMMN) of the event-related potentials (ERPs) of the brain, an index of implicit pre-attentive visual change detection, in adult humans performing an oddball-variant of change blindness flicker task. Images of 500 ms in duration were prese…

Finnish Matriculation Examination computer-based : the impact of pictures and video in assessing listening comprehension

2016

Poster at 33rd Summer School and Conference of Applied Language Studies. CoDesigns: Envisioning Multi-sited Language Education Policies. Jyväskylä, Finland, June 20-22, 2016. This research is about using visual support like videos and pictures in testing foreign language listening comprehension. The study limits to the foreign language testing in the Finnish Matriculation Examination, which is a final examination of the upper secondary school. There is a need for this research because the way people live and communicate has totally changed in this decade. The Finnish Matriculation Examination is becoming computer-based which opens new possibilities and challenges for language testing. Also …

Vārdu krājumu novērtējums ar dažādu testēšanu paņēmienu izmantošanu

2016

Darbā tika pētītas vārdu krājuma testēšanas paņēmienu priekšrocības un trūkumi un visefektīvākais un drošākais paņēmiens vārdu krājuma pārbaudei mācību apstākļos. Pētījuma rezultāti spēj veicināt izvēli par labu konkrētam testēšanas paņēmienam, saskaņā ar novērtēšanas veidu un mērķi, tēmu, mērķauditoriju un vidi. Darba teorētiskā daļa ir balstīta uz Arthur Hughes (2003), Scott Thornbury (2002), John Read (2000) un Penny Ur (1999) teorijas. Gadījuma metodes ietvaros tika vadīta mācību nodarbība, pēc kuras studentu zināšanas tika novērtētas ar autores izstrādāto testa palīdzību. Kvantitatīva analīze tika pielietota testa rezultātu noteikšanā un uzticamības mērīšanā, turklāt kvalitatīva analīz…

Word and item features that affect measurement of vocabulary size in a multiple choice vocabulary task

1999

Relations between representational consistency, conceptual understanding of the force concept, and scientific reasoning

2012

Previous physics education research has raised the question of “hidden variables” behind students’ success in learning certain concepts. In the context of the force concept, it has been suggested that students’ reasoning ability is one such variable. Strong positive correlations between students’ preinstruction scores for reasoning ability (measured by Lawson’s Classroom Test of Scientific Reasoning) and their learning of forces [measured by the Force Concept Inventory (FCI)] have been reported in high school and university introductory courses. However, there is no published research concerning the relation between students’ ability to interpret multiple representations consistently (i.e.,…