Search results for "thaliana"
showing 10 items of 136 documents
Functional analysis of endo-1,4-β-glucanases in response to Botrytis cinerea and Pseudomonas syringae reveals their involvement in plant-pathogen int…
2013
Plant cell wall modification is a critical component in stress responses. Endo-1,4-β-glucanases (EGs) take part in cell wall editing processes, e.g. elongation, ripening and abscission. Here we studied the infection response of Solanum lycopersicum and Arabidopsis thaliana with impaired EGs. Transgenic TomCel1 and TomCel2 tomato antisense plants challenged with Pseudomonas syringae showed higher susceptibility, callose priming and increased jasmonic acid pathway marker gene expression. These two EGs could be resistance factors and may act as negative regulators of callose deposition, probably by interfering with the defence-signalling network. A study of a set of Arabidopsis EG T-DNA insert…
Expression of a vegetative-storage-protein gene from Arabidopsis is regulated by copper, senescence and ozone
2001
Emerging data suggest that the mechanisms regulating plant copper homeostasis could be implicated in stress and senescence signal transduction pathways. To gain insight into copper-modulated patterns of gene expression, copper-treated Arabidopsis thaliana (L.) Heynh. plants were analysed by mRNA differential display. The experimental conditions were selected using aggregation of ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) as a molecular sensor to monitor copper-induced oxidative stress. Two copper-induced messengers encoding a vegetative storage protein (VSP2) were isolated by this technique. Both clones differed in the length of their 3'-untranslated region according to the p…
Arabidopsis copper transport protein COPT2 participates in the crosstalk between iron deficiency responses and low phosphate signaling
2013
[EN] Copper and iron are essential micronutrients for most living organisms because they participate as cofactors in biological processes, including respiration, photosynthesis, and oxidative stress protection. In many eukaryotic organisms, including yeast (Saccharomyces cerevisiae) and mammals, copper and iron homeostases are highly interconnected; yet, such interdependence is not well established in higher plants. Here, we propose that COPT2, a high-affinity copper transport protein, functions under copper and iron deficiencies in Arabidopsis (Arabidopsis thaliana). COPT2 is a plasma membrane protein that functions in copper acquisition and distribution. Characterization of the COPT2 expr…
Functional characterization of the plastidial 3-phosphoglycerate dehydrogenase family in Arabidopsis.
2013
This work contributes to unraveling the role of the phosphorylated pathway of serine (Ser) biosynthesis in Arabidopsis (Arabidopsis thaliana) by functionally characterizing genes coding for the first enzyme of this pathway, 3-phosphoglycerate dehydrogenase (PGDH). We identified two Arabidopsis plastid-localized PGDH genes (3-PGDH and EMBRYO SAC DEVELOPMENT ARREST9 [EDA9]) with a high percentage of amino acid identity with a previously identified PGDH. All three genes displayed a different expression pattern indicating that they are not functionally redundant. pgdh and 3-pgdh mutants presented no drastic visual phenotypes, but eda9 displayed delayed embryo development, leading to aborted emb…
Molecular characterization and evolution of the protein phosphatase 2A B' regulatory subunit family in plants.
2002
Abstract Type 2A serine/threonine protein phosphatases (PP2A) are important components in the reversible protein phosphorylation events in plants and other organisms. PP2A proteins are oligomeric complexes constituted by a catalytic subunit and several regulatory subunits that modulate the activity of these phosphatases. The analysis of the complete genome of Arabidopsis allowed us to characterize four novel genes, AtB′ε, AtB′ζ,AtB′η, and AtB′θ, belonging to the PP2A B′ regulatory subunit family. Because four genes of this type had been described previously, this family is composed of eight members. Reverse transcriptase-polymerase chain reaction experiments showed thatAtB′ε mRNAs are prese…
Improved Mesophyll Protoplast Culture and Plant Regeneration in Arabidopsis thaliana (L.) Heynh., Genotype Landsberg Erecta
1993
Summary The response of in vitro cultured Arabidopsis thaliana mesophyll protoplasts was investigated with the aim of characterising the crucial factors that affect plating efficiencies and plant regeneration. We selected the genotype Landsberg erecta, which is frequently used for genetic studies, but which is known to respond poorly in protoplast culture. Factors permitting vigorous vegetative growth of the donor plants, i.e. short light period and avoidance of high temperature, were found to be advantageous. Selection of competent leaves and fractionation of protoplast populations indicated that predominantly protoplasts derived from still expanding, yet not highly immature mesophyll cell…
The reduction of selenium(IV) by boreal Pseudomonas sp. strain T5-6-I – Effects on selenium(IV) uptake in Brassica oleracea
2019
Selenium (Se) is an essential micronutrient but toxic when taken in excessive amounts. Therefore, understanding the metabolic processes related to selenium uptake and bacteria-plant interactions coupled with selenium metabolism are of high importance. We cultivated Brassica oleracea with the previously isolated heterotrophic aerobic Se(IV)-reducing Pseudomonas sp. T5-6-I strain to better understand the phenomena of bacteria-mediated Se(IV) reduction on selenium availability to the plants. B. oleracea grown on Murashige and Skoog medium (MS-salt agar) with and without of Pseudomonas sp. were amended with Se(IV)/75Se(IV), and selenium transfer into plants was studied using autoradiography and…
The cytosolic Arabidopsis thaliana cysteine desulfurase ABA3 delivers sulfur to the sulfurtransferase STR18
2020
ABSTRACTThe biosynthesis of many sulfur-containing molecules depends on cysteine as a sulfur source. Cysteine desulfurase (CD) and rhodanese (Rhd) domain-containing protein families participate in the trafficking of sulfur for various metabolic pathways in bacteria and human, but their connection is not yet described in plants. The existence of natural chimeric proteins, however, containing both CD and Rhd domains in specific bacterial genera suggests a general interaction between both proteins. We report here the biochemical relationships between two cytosolic proteins from Arabidopsis thaliana, a Rhd domain containing protein, the sulfurtransferase 18 (STR18), and a CD isoform referred to…
Identification of Trans-Golgi Network Proteins in Arabidopsis thaliana Root Tissue
2014
Knowledge of protein subcellular localization assists in the elucidation of protein function and understanding of different biological mechanisms that occur at discrete subcellular niches. Organelle-centric proteomics enables localization of thousands of proteins simultaneously. Although such techniques have successfully allowed organelle protein catalogues to be achieved, they rely on the purification or significant enrichment of the organelle of interest, which is not achievable for many organelles. Incomplete separation of organelles leads to false discoveries, with erroneous assignments. Proteomics methods that measure the distribution patterns of specific organelle markers along densit…
Tráfico intracelular de proteínas de la familia p24 en células vegetales
2014
Las proteínas p24 constituyen una familia única de proteínas transmembrana de tipo I, con un peso molecular aproximado de 24 kDa, que se localizan mayoritariamente en los compartimentos de la vía secretora temprana, retículo endoplásmico (ER) y complejo de Golgi, y son componentes mayoritarios de las vesículas recubiertas por proteínas COPI o COPII. Se dividen, por homología de secuencia, en cuatro subfamilias: p24α, p24β, p24δ y p24γ. En animales y levaduras hay representantes de cada una de las 4 subfamilias; sin embargo, las plantas solo tienen miembros de la subfamilia p24β y p24δ. En Arabidopsis existen 9 miembros de la subfamilia p24δ (p24δ3-p24δ11) y 2 de la subfamilia p24β (p24β2 y …