Search results for "throughput"

showing 10 items of 483 documents

Molecular community and population studies of arbuscular mycorrhizal fungi (Glomeromycota)

2014

The arbuscular mycorrhizal symbiosis, which appeared at the same time as land plants, 460 million years ago, is a mutualistic beneficial association between most land plants, including those cultivated, and arbuscular mycorrhizal fungi (AMF). AMF, from the Glomeromycota phylum, are widespread soil microorganisms needing a photosynthetic host to complete their life cycle (obligate symbionts). The great potential of plant mineral nutrition improvement and crop production increased during this symbiosis, make AMF an asset in the context of an increase in the demand of world food crop production. The control of that symbiosis by ecology engineering in order to improve ecosystem services, especi…

Gloméromycètes[SDV.SA] Life Sciences [q-bio]/Agricultural sciencesHigh-throughput sequencingCultural practicesPopulationPopulationsCommunityPolymorphisme de longueur de fragments de restrictionSéquençage haut-débitSymbiose mycorhizienneRhizophagus irregularisArbuscular mycorrhizaPratiques culturalesGlomeromycotaRestriction fragment length polymorphismCommunauté
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The baculovirus display technology--an evolving instrument for molecular screening and drug delivery.

2008

High throughput screening is a core technology in drug discovery. During the past decade, several strategies have been developed to screen (poly)peptide libraries for diverse applications including disease diagnosis and profiling, imaging, as well as therapy. The recently established baculovirus display vector system (BDVS) represents a eukaryotic screening platform that combines the positive attributes of both cell and virus-based display approaches, allowing presentation of complex polypeptides on cellular and viral surfaces. Compared to microbial display systems, the BDVS has the advantage of correct protein folding and post-translational modifications similar to those in mammals, facili…

GlycosylationInsectaHigh-throughput screeningCellGenetic VectorsDrug Evaluation PreclinicalHeterologousComputational biologyBiologychemistry.chemical_compoundAdjuvants ImmunologicDrug DiscoverymedicineAnimalsHumansGenomic libraryGeneGene LibraryDrug discoveryOrganic ChemistryGene Transfer TechniquesGeneral MedicineMolecular biologyComputer Science Applicationsmedicine.anatomical_structurechemistryDrug deliveryBaculoviridaeCombinatorial chemistryhigh throughput screening
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Selective MicroRNA-Offset RNA Expression in Human Embryonic Stem Cells

2015

Small RNA molecules, including microRNAs (miRNAs), play critical roles in regulating pluri-potency, proliferation and differentiation of embryonic stem cells. miRNA-offset RNAs (moRNAs) are similar in length to miRNAs, align to miRNA precursor (pre-miRNA) loci and are therefore believed to derive from processing of the pre-miRNA hairpin sequence. Recent next generation sequencing (NGS) studies have reported the presence of moRNAs in human neurons and cancer cells and in several tissues in mouse, including pluripotent stem cells. In order to gain additional knowledge about human moRNAs and their putative development-related expression, we applied NGS of small RNAs in human embryonic stem cel…

HUMAN DICERSmall RNAHuman Embryonic Stem CellsMolecular Sequence Datalcsh:MedicineGene ExpressionBiologyPLURIPOTENCYCell LinemicroRNAGene expressionmiRNA-offset RNAsELEMENTSHumansSmall nucleolar RNAlcsh:ScienceInduced pluripotent stem cellGene LibraryGENE-EXPRESSIONGeneticsBinding SitesMultidisciplinaryBase Sequenceta1184Gene Expression ProfilingMATURE MICRORNASMORNASlcsh:RComputational BiologyHigh-Throughput Nucleotide SequencingRNAMolecular Sequence AnnotationRNA sequencingembryonic stem cellsEmbryonic stem cellmicroRNAsCell biologyMicroRNAsMIRNASDISCOVERYMOUSE ES CELLSRNA Small Untranslatedlcsh:Q3111 BiomedicineRNA extractionFEEDER CELLSSequence AlignmentResearch ArticlePLOS ONE
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Start-Up of Chitosan-Assisted Anaerobic Sludge Bed Reactors Treating Light Oxygenated Solvents under Intermittent Operation

2021

Quality of the granular sludge developed during the start-up of anaerobic up-flow sludge bed reactors is of crucial importance to ensure the process feasibility of treating industrial wastewater such as those containing solvents. In this study, the microbial granule formation from suspended-growth biomass was investigated in two chitosan-assisted reactors. These reactors operated mimicking industrial sites working with night closures treating a mixture of ethanol, ethyl acetate, and 1-ethoxy-2-propanol. Each reactor operated under different hydrodynamic regimes typical from UASB (R1: &lt

Health Toxicology and Mutagenesis0208 environmental biotechnologyEthyl acetate02 engineering and technology010501 environmental sciencesWaste Disposal Fluid01 natural sciencescomplex mixturesArticleIndustrial wastewater treatmentchemistry.chemical_compoundGranulationBioreactorsgranulationAcetobacteriumAigües residualsAnaerobiosis0105 earth and related environmental sciencesSewagebiologyChemistryGranule (cell biology)RPublic Health Environmental and Occupational Healthtechnology industry and agriculturehigh-throughput sequencingbiology.organism_classificationPulp and paper industryequipment and suppliesDesulfovibriointermittent feedingInnovacions tecnològiques020801 environmental engineeringsolventsMedicineEnginyeria ambientalanaerobic reactorschitosanAnaerobic exerciseGeobacterInternational Journal of Environmental Research and Public Health
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TAF-ChIP: an ultra-low input approach for genome-wide chromatin immunoprecipitation assay

2019

The authors present a novel method for obtaining chromatin profiles from low cell numbers without prior nuclei isolation. The method is successfully implemented in generating epigenetic profile from 100 cells with high signal-to-noise ratio.

Health Toxicology and MutagenesisPlant ScienceComputational biologySignal-To-Noise RatioBiochemistry Genetics and Molecular Biology (miscellaneous)GenomeDNA sequencingEpigenesis GeneticHistones03 medical and health sciences0302 clinical medicineTranscriptional regulationMethodsAnimalsHumansEpigenetics030304 developmental biologyWhole genome sequencing0303 health sciencesEcologybiologyWhole Genome SequencingChemistryHigh-Throughput Nucleotide SequencingChip11Histonebiology.proteinChromatin Immunoprecipitation SequencingDrosophilaK562 CellsChromatin immunoprecipitation030217 neurology & neurosurgerySoftwareLife Science Alliance
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Revisit of RTS/CTS Exchange in High-Speed IEEE 802.11 Networks

2005

IEEE 802.11 medium access control (MAC), called distributed coordination function (DCF), provides two different access modes, namely, 2-way (basic access) and 4-way (RTS/CTS) handshaking. The 4-way handshaking has been introduced in order to combat the hidden terminal phenomenon. It has been also proved that such a mechanism can be beneficial even in the absence of hidden terminals, because of the collision time reduction. We analyze the effectiveness of the RTS/CTS access mode, in current 802.11b and 802.11a networks. Since the rates employed for control frame transmissions can be much lower than the rate employed for data frames, the assumption on the basis of the 4-way handshaking introd…

Hidden node problembusiness.industryComputer scienceReal-time computingFrame (networking)Wireless local area networks (WLAN)Distributed coordination functionThroughputbackoff algorithmHandshakingMedia access controlMultiple Access with Collision Avoidance for WirelessIEEE 802.11e-2005businessCarrier sense multiple access with collision avoidanceComputer networkSixth IEEE International Symposium on a World of Wireless Mobile and Multimedia Networks
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Phenotyping plant root and their interaction with soil micro-organisms

2014

International audience

High Throughput Plant Phenotyping Platform (PPHD)[SDV] Life Sciences [q-bio][SDE] Environmental Sciencesplant microorganism relationshipsnodulated root imaging;[SDV]Life Sciences [q-bio][SDE]Environmental Sciences[SDV.BV]Life Sciences [q-bio]/Vegetal Biology[SDV.BV] Life Sciences [q-bio]/Vegetal BiologyComputingMilieux_MISCELLANEOUS
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Roadmap on digital holography [Invited]

2021

This Roadmap article on digital holography provides an overview of a vast array of research activities in the field of digital holography. The paper consists of a series of 25 sections from the prominent experts in digital holography presenting various aspects of the field on sensing, 3D imaging and displays, virtual and augmented reality, microscopy, cell identification, tomography, label-free live cell imaging, and other applications. Each section represents the vision of its author to describe the significant progress, potential impact, important developments, and challenging issues in the field of digital holography.

HolografiaComputer scienceCèl·lulesCellsHolography02 engineering and technology01 natural sciencesField (computer science)Visualització tridimensional010309 opticsOpticsImaging Three-DimensionalComputer graphics (images)Lab-On-A-Chip Devices0103 physical sciencesMedical imagingAnimalsHumansTomographyPotential impactbusiness.industryVirtual RealityMicrofluidic Analytical Techniques021001 nanoscience & nanotechnologyAtomic and Molecular Physics and OpticsHigh-Throughput Screening AssaysAugmented realityThree-dimensional display systems0210 nano-technologybusinessDigital holographyAlgorithms
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Parallelized short read assembly of large genomes using de Bruijn graphs

2011

Abstract Background Next-generation sequencing technologies have given rise to the explosive increase in DNA sequencing throughput, and have promoted the recent development of de novo short read assemblers. However, existing assemblers require high execution times and a large amount of compute resources to assemble large genomes from quantities of short reads. Results We present PASHA, a parallelized short read assembler using de Bruijn graphs, which takes advantage of hybrid computing architectures consisting of both shared-memory multi-core CPUs and distributed-memory compute clusters to gain efficiency and scalability. Evaluation using three small-scale real paired-end datasets shows tha…

Hybrid genome assemblyParallel computingComputational biologyBiologylcsh:Computer applications to medicine. Medical informaticsBiochemistryAssemblersStructural BiologyHumansThroughput (business)Molecular Biologylcsh:QH301-705.5De Bruijn sequenceGenomeContigBacteriaGenome HumanApplied MathematicsMessage passingDNA sequencing theoryComputational BiologyHigh-Throughput Nucleotide SequencingComputer Science Applicationslcsh:Biology (General)comic_booksScalabilitylcsh:R858-859.7comic_books.characterSoftwareResearch ArticleBMC Bioinformatics
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Analysis of the IEEE 802.11e EDCA Under Statistical Traffic

2006

Many models have been proposed to analyze the performance of the IEEE 802.11 distributed coordination function (DCF) and the IEEE 802.11e enhanced distributed coordination function (EDCA) under saturation condition. To analyze DCF under statistical traffic, Foh and Zukerman introduce a model that uses Markovian Framework to compute the throughput and delay performance. In this paper, we analyze the protocol service time of EDCA mechanism and introduce a model to analyze EDCA under statistical traffic using Markovian Framework. Using this model, we analyze the throughput and delay performance of EDCA mechanism under statistical traffic.

IEEE 802Computer sciencebusiness.industryQuality of serviceComputerSystemsOrganization_COMPUTER-COMMUNICATIONNETWORKSMarkov processWireless local area networks (WLAN)ThroughputDistributed coordination functionThroughputComputer Science::OtherComputer Science::Performancebackoff algorithmsymbols.namesakeWireless lanComputer Science::Networking and Internet ArchitecturesymbolsbusinessThroughput (business)Protocol (object-oriented programming)Computer network
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