Search results for "tissue engineering."

Growth kinetics and characterization of human dental pulp stem cells: Comparison between third molar and first premolar teeth

2015

Background Dental pulp stem cells (DPSCs) play an important role in tissue regeneration. This study compares the growth kinetics and characterization of third molar and first premolar human DPSCs. Material and Methods Dental pulp tissues were isolated from human first premolar and third molar teeth and were digested by treating them with collagenase type I. Single-cell suspensions from each dental pulp were seeded in T25 culture flasks and the media were replaced every 3 days until 70% confluence. The cells were enumerated to determine the population doubling time (PDT). Cells were characterized using flow cytometry, RT-PCR and osteogenic medium for differentiation of DPSCs. Karyotyping ass…

Biomaterials and bioactive molecules to drive differentiation in striated muscle tissue engineering

2015

International audience; The generation of engineered tissues and organs has entered into the clinical practice in response to the chronic lack of organ donors. In particular, for the skeletal and cardiac muscles the translational potential of tissue engineering approaches has clearly been shown, even though the construction of these tissues lags behind others given the hierarchical, highly organized architecture of striated muscles. Failure of the cardiac tissue leads to cardiovascular diseases, which are the leading cause of death in the developed world (Di Felice et al., 2014). On the other hand, there are many clinical cases where the loss of skeletal muscle due to a traumatic injury, an…

High-density ZnO Nanowires as a Reversible Myogenic-Differentiation-Switch

2018

Mesoangioblasts are outstanding candidates for stem-cell therapy and are already being explored in clinical trials. However, a crucial challenge in regenerative medicine is the limited availability of undifferentiated myogenic progenitor cells because growth is typically accompanied by differentiation. Here reversible myogenic-differentiation switching during proliferation is achieved by functionalizing the glass substrate with high-density ZnO nanowires (NWs). Specifically, mesoangioblasts grown on ZnO NWs present a spherical viable undifferentiated cell state without lamellopodia formation during the entire observation time (8 days). Consistently, the myosin heavy chain, typically express…

Physical and biological properties of electrospun poly( d , l ‐lactide)/nanoclay and poly( d , l ‐lactide)/nanosilica nanofibrous scaffold for bone t…

2021

Electrospun scaffolds exhibiting high physical performances with the ability to support cell attachment and proliferation are attracting more and more scientific interest for tissue engineering applications. The inclusion of inorganic nanoparticles such as nanosilica and nanoclay into electrospun biopolymeric matrices can meet these challenging requirements. The silica and clay incorporation into polymeric nanofibers has been reported to enhance and improve the mechanical properties as well as the osteogenic properties of the scaffolds. In this work, for the first time, the physical and biological properties of polylactic acid (PLA) electrospun mats filled with different concentrations of n…

Engineered axon tracts within tubular biohybrid scaffolds

2021

[EN] Injuries to the nervous system that involve the disruption of axonal pathways are devastating to the individual and require specific tissue engineering strategies. Here we analyse a cells-biomaterials strategy to overcome the obstacles limiting axon regeneration in vivo, based on the combination of a hyaluronic acid (HA) single-channel tubular conduit filled with poly-L-lactide acid (PLA) fibres in its lumen, with pre-cultured Schwann cells (SCs) as cells supportive of axon extension. The HA conduit and PLA fibres sustain the proliferation of SC, which enhance axon growth acting as a feeder layer and growth factor pumps. The parallel unidirectional ensemble formed by PLA fibres and SC …

Optimization of a decellularized protocol of porcine tracheas. Long-term effects of cryopreservation. A histological study

2021

[EN] Objective: The aim of this study was to optimize a decellularization protocol in the trachea of Sus scrofa domestica (pig) as well as to study the effects of long-term cryopreservation on the extracellular matrix of decellularized tracheas. Methods: Porcine tracheas were decellularized using Triton X-100, SDC, and SDS alone or in combination. The effect of these detergents on the extracellular matrix characteristics of decellularized porcine tracheas was evaluated at the histological, biomechanical, and biocompatibility level. Morphometric approaches were used to estimate the effect of detergents on the collagen and elastic fibers content as well as on the removal of chondrocytes from …

Bioengineering strategies of the uterus towards improving current investigative models and female reproductive health

2018

In this thesis, we intended to develop tissue-engineering approaches based on decellularized (DC) uterine tissues obtained from whole organs to improve several aspects within reproductive medicine. We hypothesized that that the decellularization of whole uteri from different species has not only the potential to, one day, create tissue-engineered, transplantable organs but that the DC endometrial fraction can also be processed further into thin sections, ECM hydrogels and coatings that can be used as a biocompatible tissue-specific substrate for cell and embryo culture. Moreover, we intended to corroborate if the differences in the cyclically and drastically changing endometrium are transla…

BIODEGRADABLE POLYASPARTAMIDE-GRAFT-POLYESTER COPOLYMER FOR VASCULAR REGENERATION

2012

Use of decellularized tracheas for airways engineering

2016

Background/Objective: Tracheal stenosis produces serious dyspnea. In some cases, a defective correction or timely intervention can lead to the death of the patient. The Hospital Clinico Universitario de Valencia is a reference hospital for the treatment of patients with airway problems exhibiting complex tracheal stenosis. Surgery is limited to a maximum length of 6-7 cm and it is not exempt of mortality (3-5%). In this study we have study the useful of decellularized porcine tracheas to be used in airway regenerative medicine. Methods: Three porcine tracheas were cut in 3 cm rings and exposed to SDS 2% for up to four weeks. Cell content was evaluated by fluorescence microscopy (DAPI) weekl…

Wharton's Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration

2013

Abstract Perinatal stem cells such as human umbilical cord Wharton's jelly stem cells (HWJSCs) are excellent candidates for tissue engineering because of their proliferation and differentiation capabilities. However, their differentiation potential into epithelial cells at in vitro and in vivo levels has not yet been reported. In this work we have studied the capability of HWJSCs to differentiate in vitro and in vivo to oral mucosa and skin epithelial cells using a bioactive three-dimensional model that mimics the native epithelial-mesenchymal interaction. To achieve this, primary cell cultures of HWJSCs, oral mucosa, and skin fibroblasts were obtained in order to generate a three-dimension…