Search results for "torquetenovirus"

showing 3 items of 3 documents

Assessment of prevalence and load of torquetenovirus viraemia in a large cohort of healthy blood donors.

2020

OBJECTIVES: Torquetenovirus (TTV) is an emerging marker of functional immune competence with the potential to predict transplant-related adverse events. A large-scale epidemiological study was performed to understand how basal values vary in healthy individuals according to age and gender.; METHODS: We tested plasma from 1017 healthy blood donors aged 18-69years. The presence and load of TTV were determined by a real-time PCR assay. A sub-cohort of 384 donors was tested for anti-cytomegalovirus IgG antibodies, and 100 participants were also tested for TTV viraemia on a paired whole blood sample.; RESULTS: The overall prevalence of TTV was 65% (657/1017) with a mean (±SD) growth of 5±4% ever…

0301 basic medicineMicrobiology (medical)AdultMalemedicine.medical_specialtyAgingAdolescentprevalence030106 microbiologyPcr assayPhysiologyTTVViremiaBlood DonorsanelloviridaeReal-Time Polymerase Chain Reaction03 medical and health sciencesPlasmaYoung Adult0302 clinical medicineEpidemiologyMedicineHumansBlood Transfusion030212 general & internal medicineViremiaTTV; anelloviridae; blood donors; healthy controls; prevalence; torquetenovirus; viremiaAdverse effectWhole bloodAgedTorque teno virusbiologybusiness.industryGeneral MedicineMiddle AgedViral Loadmedicine.diseaseDNA Virus InfectionsHealthy VolunteersLarge cohorttorquetenovirusInfectious DiseasesHealthy individualsDNA Viralbiology.proteinhealthy controlsFemaleAntibodybusinessClinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
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Comparative evaluation of molecular methods for the quantitative measure of torquetenovirus (TTV) viremia, the new surrogate marker of immune compete…

2019

Background Torquetenovirus (TTV) viremia is emerging as a promising tool to assess functional immune competence, to predict posttransplant immune-related complications, and eventually to customize immunosuppression. Methods In this study, 327 blood samples were tested using two real-time PCR (rtPCR) assays both targeted to the untranslated region of the TTV genome. The first assay was an in-house rtPCR developed by our group, the second one was the recently marketed TTV R-GENE assay. Results In the validation study, the TTV R-GENE showed good performances in precision and reproducibility, and sensitivity as low as 12 TTV DNA copies/mL, like previously reported for the in-house rtPCR. The Bl…

Concordancedigital droplet PCR; methods comparison; real-time PCR; torquetenovirusViremiaTTVBiologyReal-Time Polymerase Chain ReactionComparative evaluation03 medical and health sciencesTTV; digital droplet PCR; methods comparison; real-time PCR0302 clinical medicineImmune systemVirologymedicineHumans030212 general & internal medicineViremiaDigital droplet pcrTorque teno virusSurrogate endpointReproducibility of Resultsmedicine.diseaseVirologyDNA Virus InfectionsQuantitative measuretorquetenovirusInfectious DiseasesReal-time polymerase chain reactionCase-Control StudiesDNA Viral030211 gastroenterology & hepatologyreal-time PCRImmunocompetencedigital droplet PCRBiomarkersmethods comparison
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Valor predictivo de la carga viral de Citomegalovirus y Torquetenovirus en heces y plasma del desarrollo de enfermedad de injerto contra huésped inte…

2022

Uno de los retos clínicos más relevantes en los pacientes sometidos a trasplante alogénico de precursores hematopoyéticos (alo-TPH) es reducir el riesgo de enfermedad de injerto contra huésped (EICH). Otro de estos, es evitar la infección por Citomegalovirus (CMV) debido a su elevada morbilidad y mortalidad. En este contexto, se ha postulado una posible relación recíproca entre el riesgo de EICH y la infección por CMV, aunque existen datos contradictorios en la literatura a este respecto, especialmente al relacionar la presencia de CMV en heces, como predictor de EICH de tipo intestinal. Adicionalmente, el Torque Teno Virus (TTV) se encuentra actualmente bajo estudio debido a su íntima rela…

torquetenovirusEICHaialo-tphUNESCO::CIENCIAS MÉDICASUNESCO::CIENCIAS DE LA VIDAcitomegalovirus:CIENCIAS MÉDICAS [UNESCO]:CIENCIAS DE LA VIDA [UNESCO]
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