Search results for "toxin"

Reduction of the enniatins A, A1, B, B1 by an in vitro degradation employing different strains of probiotic bacteria: Identification of degradation p…

2013

Abstract The degradation of the Fusarium mycotoxins ENs by 9 bacterial strains characteristic of the gastrointestinal tract like Bb. longum , Bb. bifidum , Bb. breve , Bb. adolescentes , Lb. rhamnosus , Lb. casei–casei , S. termofilus , Lb. ruminis , Lb. casei and twenty two strains of Saccharomyces cerevisiae was studied. The fermentations were carried out in the liquid medium of De Man Rogosa Sharpe (MRS) under anaerobic conditions for Bifidobacteria Streptococcus and Lactobacillus, and in Potato Dextrose Broth (PDB) for Saccharomyces strains, during 48 h. The degradation of the bioactive compounds ENs was also studied in a food system composed by wheat flour naturally contaminated by ENs…

Determination of Fusarium mycotoxins enniatins, beauvericin and fusaproliferin in cereals and derived products from Tunisia

2011

Abstract In this study, 51 samples of cereals (wheat, Barley, maize and Sorghum) and by-products (mainly pasta and couscous) purchased from Tunisian supermarkets were examined for contamination with the emerging Fusarium mycotoxins: Enniatins ENs (EN A, EN A 1 , EN B and EN B 1 ), beauvericin (BEA) and fusaproliferin (FUS). The extraction of the samples was performed with methanol using an Ultra-turrax homogenizer. Mycotoxins were analyzed with a liquid chromatography (LC) coupled to a diode array detector (DAD). The frequencies of contamination of total samples with ENs were 96%. EN A 1 was the most common EN found with the highest prevalence of 92.1%, levels ranged between 11.1 and 480 mg…

Challenges of Lactobacillus fermentation in combination with acoustic screening for deoxynivalenol and deoxynivalenol conjugates reduction in contami…

2022

Abstract This study was dedicated to apply biological treatment using Lactobacillus (LAB) fermentation separately or in combination with an acoustic screening method for the prevention of mycotoxins in Fusarium spp. contaminated wheat-based products. Wheat samples of different contamination were treated separately using antimicrobial LAB strains (L. casei, L. plantarum, L. paracasei, and L. uvarum) and the changes on the level of deoxynivalenol (DON) and its conjugates such as 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), and DON-3-β-D-glucoside (D3G)) were evaluated using UHPLC-QqQ-MS/MS and UHPLC-Orbitrap-HRMS. Additionally, an acoustic device was used to analyse DON…

Comparison of toxic effects on CHO-KL and VERO cells after individual and combined treatment with Fusarium mycotoxins, beauvericin, deoxynivalenol an…

2009

Degradation of the minor Fusarium mycotoxin beauvericin by intracellular enzymes of Saccharomyces cerevisiae

2013

Abstract Beauvericin (BEA) is a cyclic depsipeptide with antibiotic and insecticidal effects. It was discovered for the first time from the fungus Beauveria bassiana , but more significantly, is produced by several Fusarium strains, and considered a contaminant of several cereals like corn, wheat and barley. This study investigated the degradation of BEA by intracellular raw enzymes of four strains of Saccharomyces cerevisiae , named LO9, YE5, A34, and A17. The BEA at 25 mg/kg in a model solution and in corn flour was co-incubated with the raw enzymes from the four yeast strains, respectively. The reduction of BEA was evaluated using liquid chromatography coupled to a diode array detector (…

First report on the presence of emerging Fusarium mycotoxins enniatins (A, A1, B, B1), beauvericin and fusaproliferin in rice on the Moroccan retail …

2011

Seventy samples of rice purchased from local markets in six cities from Morocco (Rabat, Casablanca, Kenitra, Mohammadia, Tanger and Errachidia) were analyzed for the presence of six emerging mycotoxins: four enniatins ENs (ENA, ENA1, ENB and ENB1), beauvericin (BEA) and fusaproliferin (FUS). Samples were extracted with a mixture of acetonitrile/water (85/15, v/v) by using an ultra-turrax homogenizer. Mycotoxins were then identified and quantified with liquid chromatography (LC) coupled to diode array detector (DAD). Positive samples were confirmed with an LC-MS/MS. Analytical results showed that BEA was present in 75.7% of total analyzed samples. BEA levels varied between 3.8 and 26.3 mg/kg…

Further data on the occurrence of Fusarium emerging mycotoxins enniatins (A, A1, B, B1), fusaproliferin and beauvericin in raw cereals commercialized…

2011

Abstract In this study, 64 samples of raw cereals (wheat, maize and barley) purchased from local markets in Rabat–Sale area from Morocco were analyzed for the occurrence of six emerging mycotoxins: four enniatins ENs (ENA, ENA1, ENB and ENB1), beauvericin (BEA) and fusaproliferin (FUS). Samples were extracted with a mixture of water/acetonitrile (85/15, v/v) by using an Ultra-turrax homogenizer. Mycotoxins were then identified and quantified with a liquid chromatography (LC) with diode array detector (DAD). Positive samples were confirmed with an LC–MS/MS. Analytical results showed that the frequencies of contamination of total samples with ENs, BEA and FUS were 50, 26.5 and 7.8%, respectiv…

Fluorescent probes on HepG2 cells treated with emergent Fusarium mycotoxins

2012

Identification by PCR of Trichothecene-ProducingFusarium

2008

Occurrence of fumonisins in foods

2004

Fumonisins (FB) are synthesized mainly by different species of the genus Fusarium and FB1 is the predominating fumonisin isolated in foods. FB1 shows different toxicological effects in humans and animals and has been associated with esophageal cancer in different countries. This review presents an overview of the worldwide occurrence of fumonisins in crops and human foods being corn and corn meal the most contaminated foods, and reviews variations of the presence of these mycotoxins in foods and in agricultural crops. In foods, these variations are due to the alkaline solutions, water and temperature. For agricultural crops, the role of climatic conditions and latitude, genotypes, spoilage …