Search results for "toy"

showing 10 items of 324 documents

Flow cytometric analysis of glyoxalase-1 expression in human leukocytes.

2011

Altered glyoxalase-1 (GLO-1) activity and expression is associated with the development of late diabetic complications, malignancy and oxidative stress- and aging-related diseases. In the present study, we developed a flow cytometry method for GLO-1 detection in human leukocytes isolated from peripheral blood samples to investigate GLO-1 expression in leukocyte subsets from type 1 and 2 diabetes mellitus patients (n = 11) and healthy subjects (n = 8). The flow cytometry analysis of GLO-1 in leukocytes showed that expression index of GLO-1-positive cells was slightly increased in mononuclear leukocytes from diabetic patients. This result correlated with the increase in GLO-1 activity in the …

AdultMaleClinical BiochemistryType 2 diabetesBiochemistryGene Expression Regulation EnzymologicFlow cytometryPathogenesisYoung AdultGlycationDiabetes mellitusDiabetes MellitusMedicineHumansCells CulturedWhole bloodAgedmedicine.diagnostic_testbusiness.industryExpression indexLactoylglutathione LyaseCell BiologyGeneral MedicineMiddle Agedmedicine.diseaseFlow CytometryCase-Control StudiesImmunologyLeukocytes MononuclearFemalebusinessGlyoxalase systemCell biochemistry and function
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Identification of glyoxalase 1 polymorphisms associated with enzyme activity.

2013

The glyoxalase system and its main enzyme, glyoxalase 1 (GLO1), protect cells from advanced glycation end products (AGEs), such as methylglyoxal (MG) and other reactive dicarbonyls, the formation of which is increased in diabetes patients as a result of excessive glycolysis. MG is partly responsible for harmful protein alterations in living cells, notably in neurons, leading to their dysfunction, and recent studies have shown a negative correlation between GLO1 expression and tissue damage. Neuronal dysfunction is a common diabetes complication due to elevated blood sugar levels, leading to high levels of AGEs. The aim of our study was to determine whether single nucleotide polymorphisms (S…

AdultMalemedicine.medical_specialtyGenotypeType 2 diabetesPolymorphism Single Nucleotidechemistry.chemical_compoundEnzyme activatorLactoylglutathione lyaseInternal medicineDiabetes mellitusGeneticsmedicineHumansAllelesGeneticsType 1 diabetesbiologyMethylglyoxalLactoylglutathione LyaseGeneral MedicineMiddle Agedmedicine.diseaseEnzyme assayEnzyme ActivationEndocrinologyDiabetes Mellitus Type 1chemistryDiabetes Mellitus Type 2biology.proteinFemaleGlyoxalase systemGene
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Association of reduced glyoxalase 1 activity and painful peripheral diabetic neuropathy in type 1 and 2 diabetes mellitus patients

2013

Abstract Aims The present study was undertaken to investigate the relationship between glyoxalase 1 (Glo1) enzyme activity and painful diabetic neuropathy (DN) in patients with diabetes mellitus. Methods Glo1 activity and biochemical markers were determined in blood samples from 108 patients with type 1 diabetes, 109 patients with type 2 diabetes, and 132 individuals without diabetes as a control. Painful and painless peripheral DN was assessed and multivariate regression analysis was used to determine independent association of Glo1 activity with occurrence of painful DN. Results In patients with type 1 and type 2 diabetes mellitus and painful DN compared to patients with painless DN, Glo1…

AdultMalemedicine.medical_specialtyMultivariate analysisEndocrinology Diabetes and MetabolismPainType 2 diabetesSeverity of Illness IndexGastroenterologyEndocrinologyDiabetic NeuropathiesInternal medicineDiabetes mellitusInternal MedicinemedicineHumansPain MeasurementGlycated HemoglobinType 1 diabetesbusiness.industryConfoundingLactoylglutathione LyasePeripheral Nervous System DiseasesType 2 Diabetes MellitusMiddle Agedmedicine.diseasePeripheralDiabetes Mellitus Type 1EndocrinologyDiabetes Mellitus Type 2Painful diabetic neuropathyDisease ProgressionFemalebusinessBiomarkersJournal of Diabetes and its Complications
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Current state of clinical and morphological features in human NCL.

2004

The neuronal ceroid lipofuscinoses (NCL) are large group of autosomal recessive lysosomal storage disorders with both enzymatic deficiency and structural protein dysfunction. Previously, diagnosis of (NCL) was based on age at onset clinicopathological (C‐P) findings described 4 forms, classified as infantile (INCL) (2), late‐infantile (LINCL) (5), juvenile (JNCL) (6), and adult (ANCL) most patients with NCL have progressive ocular and cerebral dysfunvtion, including cognitive/motor dysfunction and uncontrolled seizures. After reviewing 520 patients with NCL, we found that about 104 (20%) did not fit this classification of NCL With further research, 4 additional forms have been recognized: F…

AdultPathologymedicine.medical_specialtymedicine.disease_causeArticlePathology and Forensic MedicineEpilepsyNeuronal Ceroid-LipofuscinosesGenotypemedicineHumansPalmitoyl protein thioesteraseAge of OnsetChildInclusion BodiesMutationbiologyTripeptidyl-Peptidase 1General NeurosciencePPT1Infantmedicine.diseasePhenotypeCLN8Child PreschoolMutationbiology.proteinNeurology (clinical)Age of onsetBrain pathology (Zurich, Switzerland)
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Intercellular Communication in Skeletal Muscle Stem Cell Niche: Focus on extracellular vesicles and secreted signals

AlixS-palmitoylationmuscleexosome; Alix; S-palmitoylation; muscle; self-renewal; intercellular communication;intercellular communicationexosomeself-renewal
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Monolayers of dipalmitoylphosphatidylcholine at the oil-water interface

1995

Abstract Monolayers of dipalmitoylphosphatidylcholine at the interfaces waterbicylohexyl, waterdodecane and waterhexadecane were studied by fluorescence microscopy and thermodynamic measurements. The experiments show that the oil also partitions in the liquid condensed (LC) monolayer phase with concentration depending on the type of oil and the lateral pressure. In contrast to monolayers of dipalmitoylphosphatidylethanolamine the larger molecular area per head group enables oil partitioning in the LC phase even if there is a mismatch between the aliphatic tails of liquid and alkane.

Alkanechemistry.chemical_classificationChromatographyDodecaneAnalytical chemistryHexadecanechemistry.chemical_compoundColloid and Surface ChemistryHydrocarbonchemistryDipalmitoylphosphatidylcholinePhase (matter)PhosphatidylcholineMonolayerColloids and Surfaces A: Physicochemical and Engineering Aspects
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Thermodynamic Study of Small Hydrophobic Ions at the Water–Lipid Interface

2001

Abstract The thermodynamics of binding of two small hydrophobic ions such as norharman and tryptophan to neutral and negatively charged small unilamellar vesicles was investigated at pH 7.4 using fluorescence spectroscopy. Vesicles were formed at room temperature from dimyristoyl phosphatidylcholine (DMPC) or DMPC/dimyristoylphosphatidic acid and DMPC/dimyristoylphosphatidylglycerol. The changes in fluorescence properties were used to obtain association isotherms at variable membrane surface negative charge and at different ionic strengths. The binding of both ions was found to be quantitatively enhanced as the percentage of negative phospholipid increases in the membrane. Also, a decrease …

Analytical chemistryPhospholipidPhosphatidic AcidsIonic bondingBiomaterialschemistry.chemical_compoundColloid and Surface ChemistryIon bindingElectrochemistryLipid bilayerUnilamellar LiposomesIonsChromatographyVesicleTryptophanBinding constantSurfaces Coatings and FilmsElectronic Optical and Magnetic MaterialsPartition coefficientHarminechemistryPartition equilibriumThermodynamicslipids (amino acids peptides and proteins)DimyristoylphosphatidylcholineHydrophobic and Hydrophilic InteractionsCarbolinesJournal of Colloid and Interface Science
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Pharmacological blockade of the fatty acid amide hydrolase (FAAH) alters neural proliferation, apoptosis and gliosis in the rat hippocampus, hypothal…

2015

Endocannabinoids participate in the control of neurogenesis, neural cell death and gliosis. The pharmacological effect of the fatty acid amide hydrolase (FAAH) inhibitor URB597, which limits the endocannabinoid degradation, was investigated in the present study. Cell proliferation (phospho-H3(+) or BrdU(+) cells) of the main adult neurogenic zones as well as apoptosis (cleaved caspase-3(+)), astroglia (GFAP(+)), and microglia (Iba1(+) cells) were analyzed in the hippocampus, hypothalamus and striatum of rats intraperitoneally treated with URB597 (0.3 mg/kg/day) at one dose/4-days resting or 5 doses (1 dose/day). Repeated URB597 treatment increased the plasma levels of the N-acylethanolamine…

AstrocitosNeurobiologia del desenvolupamentAmidohidrolasasCannabinoid receptorCarbamatos:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Intracellular Signaling Peptides and Proteins::Apoptosis Regulatory Proteins::Caspases [Medical Subject Headings]:Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Differentiation::Neurogenesis [Medical Subject Headings]medicine.medical_treatment:Chemicals and Drugs::Carbohydrates::Monosaccharides::Hexoses::Glucose [Medical Subject Headings]Apoptosis:Phenomena and Processes::Physiological Phenomena::Body Constitution::Body Weights and Measures::Body Size::Body Weight [Medical Subject Headings]chemistry.chemical_compound:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Membrane Proteins::Receptors Cell Surface::Receptors G-Protein-Coupled::Receptors Cannabinoid::Receptor Cannabinoid CB1 [Medical Subject Headings]0302 clinical medicine:Chemicals and Drugs::Organic Chemicals::Carboxylic Acids::Acids Acyclic::Carbamates [Medical Subject Headings]Fatty acid amide hydrolaseReceptor cannabinoide CB1:Organisms::Eukaryota::Animals [Medical Subject Headings]FAAHGliosishealth care economics and organizations:Chemicals and Drugs::Nucleic Acids Nucleotides and Nucleosides::Nucleosides::Deoxyribonucleosides::Deoxyuridine::Bromodeoxyuridine [Medical Subject Headings]:Chemicals and Drugs::Lipids::Glycerides::Triglycerides [Medical Subject Headings]Original Research0303 health sciencesNeurogenesisBenzamidas:Chemicals and Drugs::Polycyclic Compounds::Steroids::Cholestanes::Cholestenes::Cholesterol [Medical Subject Headings]Endocannabinoid systemEtanolaminas3. Good healthEndocannabinoides:Chemicals and Drugs::Lipids::Fatty Acids::Fatty Acids Unsaturated::Fatty Acids Monounsaturated::Oleic Acids [Medical Subject Headings]CannabinoidesMicroglíalipids (amino acids peptides and proteins)medicine.symptomColesterol:Chemicals and Drugs::Organic Chemicals::Hydrocarbons::Terpenes::Cannabinoids [Medical Subject Headings]:Chemicals and Drugs::Lipids::Fatty Acids::Palmitic Acids [Medical Subject Headings]psychological phenomena and processesProliferación celularmedicine.medical_specialtyCerebroNeurogenesiseducationBiologyBromodesoxiuridina:Anatomy::Nervous System::Neuroglia::Microglia [Medical Subject Headings]Triglicéridoslcsh:RC321-571Ácidos oléicosRatas03 medical and health sciencesCellular and Molecular NeuroscienceInternal medicineHipocampomedicineCaspasa 3:Anatomy::Nervous System::Central Nervous System::Brain::Limbic System::Hippocampus [Medical Subject Headings]:Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Growth Processes::Cell Proliferation [Medical Subject Headings]lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry030304 developmental biologyPalmitoylethanolamide:Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Molecular Mechanisms of Pharmacological Action::Neurotransmitter Agents::Endocannabinoids [Medical Subject Headings]:Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Amidohydrolases [Medical Subject Headings]Cannabinoids:Anatomy::Cells::Neuroglia::Astrocytes [Medical Subject Headings]Peso corporalEnergy metabolism:Anatomy::Nervous System::Central Nervous System::Brain [Medical Subject Headings]:Anatomy::Nervous System::Central Nervous System::Brain::Limbic System::Hypothalamus [Medical Subject Headings]URB597:Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Death [Medical Subject Headings]:Diseases::Pathological Conditions Signs and Symptoms::Pathologic Processes::Gliosis [Medical Subject Headings]:Chemicals and Drugs::Organic Chemicals::Amines::Amino Alcohols::Ethanolamines [Medical Subject Headings]Muerte celular:Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Death::Apoptosis [Medical Subject Headings]:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats [Medical Subject Headings]EndocrinologyURB597chemistryGliosisnervous systemGlucosaCannabinoidEnergy Metabolism:Chemicals and Drugs::Organic Chemicals::Amides::Benzamides [Medical Subject Headings]HipotálamoÁcidos palmíticos030217 neurology & neurosurgeryNeuroscienceFrontiers in Cellular Neuroscience
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Mitigating the Climate Change Impacts of Aviation through Behavioural Change

2020

Aviation plays a crucial role for economic development and social welfare, but at the same time it also significantly contributes to climate change. Therefore, if the industry wants to follow the same growth path as it has in the past, it will need to mitigate its environmental impacts more seriously or it may otherwise face regulatory restrictions. The current literature has discussed five mitigation strategies. These are technological changes, market-based changes, operational changes, regulatory changes and behavioural changes. While several authors have regarded behavioural changes as the measure with the greatest mitigation potential, it is also the measure that has received far less a…

Aviationbusiness.industryTechnological changePerspective (graphical)Carbon offsetClimate changeSocial WelfareGeneral Medicineenvironmental impactsEnvironmental economicsilmailuilmastonmuutoksetDilemmalentoyhtiötbehavioural changeOrder (exchange)ympäristövaikutuksetkulutustottumuksetBusinessair passengers
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Giant liposomes as model membranes for immunological studies: spontaneous insertion of purified K1-antigen (poly-alpha-2,8-NeuAc) of Escherichia coli.

1990

A flow chamber has been constructed to use giant liposomes (diameter 5-50 microns) as model membranes for immunological studies and other experiments involving the interaction with water-soluble compounds. As an example of immunological importance, the insertion of purified K-antigen from Escherichia coli K1 has been studied. Despite its large hydrophilic part (poly-alpha-2,8-NeuAc), which is capped at its potential reducing end with phosphatidic acid acting as a lipid anchor group, this water-soluble material is readily incorporated into liposomal membranes of dimyristoylphosphatidylcholine (DMPC). The incorporation has been proven by immunofluorescence using a FITC-labeled monoclonal anti…

BiophysicsFluorescent Antibody TechniqueNeuraminidaseBiologymedicine.disease_causeBiochemistryModels BiologicalResidue (chemistry)chemistry.chemical_compoundMembrane LipidsmedicineEscherichia coliMicroscopy Phase-ContrastEscherichia coliHEPESchemistry.chemical_classificationLiposomeAntigens BacterialAntibodies MonoclonalWaterCell BiologyPhosphatidic acidbiology.organism_classificationEnterobacteriaceaeEnzymeMembranechemistryBiochemistrySolubilityImmunoglobulin GAntigens SurfaceLiposomesDimyristoylphosphatidylcholineBiochimica et biophysica acta
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