Search results for "translocation"

A new method for the direct quantification of lipopolysaccharide by High Performance Liquid Chromatography coupled with Mass Spectrometry in non-infe…

2017

IF 13.246; International audience

The effect of Shallow Tillage on soil erosion in a semi-arid vineyard

2019

Soil erosion has been considered a threat for semi-arid lands due to the removal of solid materials by water and wind. Although water erosion is currently considered the most important process of soil degradation, a growing interest has been drawn to the impact of soil tillage. Although numerous studies on tillage erosion have been carried out on arable land using a moldboard plow, a chisel, and a tandem disc for different crops, there are no studies on the effect of shallow tillage on soil redistribution in vineyards. The aim of this work was to evaluate the soil tillage erosion rate in a vineyard using a 13C natural abundance tracer. A strip of soil (C3-C soil) was removed, mixed with C4-…

Instruction of haematopoietic lineage choices, evolution of transcriptional landscapes and cancer stem cell hierarchies derived from an AML1-ETO mous…

2013

The t(8;21) chromosomal translocation activates aberrant expression of the AML1-ETO (AE) fusion protein and is commonly associated with core binding factor acute myeloid leukaemia (CBF AML). Combining a conditional mouse model that closely resembles the slow evolution and the mosaic AE expression pattern of human t(8;21) CBF AML with global transcriptome sequencing, we find that disease progression was characterized by two principal pathogenic mechanisms. Initially, AE expression modified the lineage potential of haematopoietic stem cells (HSCs), resulting in the selective expansion of the myeloid compartment at the expense of normal erythro- and lymphopoiesis. This lineage skewing was foll…

Adsorption-induced polymer translocation through a nanopore: a Monte Carlo investigation

2005

Abstract We study the translocation of a coarse-grained flexible polymer through a nanopore in a membrane induced by its adsorption on the trans side of the membrane. Dramatic differences in the threading behavior are observed if the adhesion to the membrane wall, e w , is below or above the adsorption threshold e c r . For e w e c r (weak adsorption) the activation barrier for translocation is at c cis 0 ≈ N / 2 (in terms of the fraction of chain c cis = N cis / N before the pore), independent of chain length N. For e w > e c r this barrier is at a constant (vanishing) number of passed trans monomers for all N. The mean time of chain passage τ trans ∝ c cis 1.3 when c cis c cis 0 . It scal…

Translocation dynamics of a short polymer driven by an oscillating force

2013

Under the terms of the Creative Commons Attribution 3.0 Unported License.

P100 Guanosine prevents nuclear factor-κB nuclear translocation ameliorating experimental colitis in rats

2018

Trisomy 12 and translocation (7;9) in an ovarian immature teratoma.

1989

We report a case of an immature malignant ovarian teratoma with peritoneal implants diagnosed in an 18-year-old woman. The tumor was brought into remission after surgery (three laparotomies) and adjuvant chemotherapy. A residual peritoneal implant showed a mature epithelial and glial configuration. Histologically, the neuroectodermal component was dominant in the original tumor as well as in the metastases, being confirmed by immunohistochemistry and electron microscopy. A stem cell line has been obtained with cell culture, having a germ cell character and a yolk sac configuration. This line possessed a trisomy 12 and a translocation (7;9) similar to other chromosomal abnormalities describe…

Light-dependent CK2-mediated phosphorylation of centrins regulates complex formation with visual G-protein.

2008

AbstractCentrins are Ca2+-binding EF-hand proteins. All four known centrin isoforms are expressed in the ciliary apparatus of photoreceptor cells. Cen1p and Cen2p bind to the visual G-protein transducin in a strictly Ca2+-dependent way, which is thought to regulate light driven movements of transducin between photoreceptor cell compartments. These relatively slow motile processes represent a novel paradigm in light adaptation of photoreceptor cells.Here we validated specific phosphorylation as a novel regulator of centrins in photoreceptors. Centrins were differentially phosphorylated during photoreceptor dark adaptation. Inhibitor treatments revealed protein kinase CK2 as the major protein…

Intensive Management and Natural Genetic Variation in Red Deer (Cervus elaphus)

2017

The current magnitude of big-game hunting has outpaced the natural growth of populations, making artificial breeding necessary to rapidly boost hunted populations. In this study, we evaluated if the rapid increase of red deer (Cervus elaphus) abundance, caused by the growing popularity of big-game hunting, has impacted the natural genetic diversity of the species. We compared several genetic diversity metrics between 37 fenced populations subject to intensive management and 21 wild free-ranging populations. We also included a historically protected population from a national park as a baseline for comparisons. Contrary to expectations, our results showed no significant differences in geneti…

A One-Step PCR-Based Assay to Evaluate the Efficiency and Precision of Genomic DNA-Editing Tools

2017

Despite rapid progress, many problems and limitations persist and limit the applicability of gene-editing techniques. Making use of meganucleases, TALENs, or CRISPR/Cas9-based tools requires an initial step of pre-screening to determine the efficiency and specificity of the designed tools. This step remains time consuming and material consuming. Here we propose a simple, cheap, reliable, time-saving, and highly sensitive method to evaluate a given gene-editing tool based on its capacity to induce chromosomal translocations when combined with a reference engineered nuclease. In the proposed technique, designated engineered nuclease-induced translocations (ENIT), a plasmid coding for the DNA-…