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showing 10 items of 10618 documents

Cytochrome P450 2E1 variable number tandem repeat polymorphisms and health risks: A genotype-phenotype study in cancers associated with drinking and/…

2012

Cytochrome P450 2E1 (CYP2E1) is one of the main enzymes involved in the oxidation of ethanol and in the transformation of a number of potentially dangerous compounds. It has various polymorphic sites, one of which is a variable number tandem repeat (VNTR) polymorphism previously described in the 5'-flanking region. The aim of this study was to investigate the genotype-phenotype association between CYP2E1 VNTR polymorphisms and risky health habits in healthy subjects and to analyze the associations between these polymorphisms with drinking- and/or smoking-related cancers. We analyzed 166 healthy subjects by genotyping for the CYP2E1 VNTR polymorphism associated with drinking and/or smoking h…

AdultMaleCancer Researchmedicine.medical_specialtyCarcinoma HepatocellularAlcohol Drinkinghuman genetic variability genetic factors cytochrome P450 2E1 variable number tandem repeat polymorphisms predis-posing alleles health risks drinking- and/or smoking-related cancer.Minisatellite RepeatsBiologyBiochemistryGastroenterologyRestriction fragmentYoung AdultRisk-TakingRisk FactorsInternal medicineGenotypeOdds RatioGeneticsmedicineHumansGenetic Predisposition to DiseaseMolecular BiologyGenotypingGenetic Association StudiesGeneticsPolymorphism GeneticLiver NeoplasmsSmokingCytochrome P-450 CYP2E1Odds ratiomedicine.diseaseConfidence intervalPancreatic NeoplasmsVariable number tandem repeatSettore BIO/18 - GeneticaOncologyCase-Control StudiesHepatocellular carcinomabiology.proteinMolecular MedicineAdenocarcinomaFemalePolymorphism Restriction Fragment Length
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Somatostatin-producing neuroendocrine tumors of the duodenum and pancreas: incidence, types, biological behavior, association with inherited syndrome…

2008

Somatostatin-producing neuroendocrine tumors (SOM-NETs) of the duodenum and pancreas appear to be heterogeneous. To determine their clinicopathological profiles, respective data were analyzed on a series of 82 duodenal and 541 pancreatic NETs. In addition, the clinical records of 821 patients with duodenal or pancreatic NETs were reviewed for evidence of a somatostatinoma syndrome. Predominant or exclusive expression of somatostatin was found in 21 (26%) duodenal and 21 (4%) pancreatic NETs. They were classified as sporadic (n=31) or neurofibromatosis type 1 (NF1)-associated duodenal NETs (n=3), gangliocytic paragangliomas (GCPGs; n=6), or poorly differentiated neuroendocrine carcinomas (pd…

AdultMaleCancer Researchmedicine.medical_specialtyEndocrinology Diabetes and Metabolism610 Medicine & healthNeuroendocrine tumorsGastroenterologyParagangliomaEndocrinologyDuodenal NeoplasmsInternal medicine10049 Institute of Pathology and Molecular PathologySomatostatinomaMultiple Endocrine Neoplasia Type 1MedicineHumansMEN11306 Cancer ResearchGenetic Predisposition to DiseaseNeurofibromatosisMultiple endocrine neoplasia610 Medicine & healthAgedAged 80 and overbusiness.industryIncidenceSyndromeSomatostatinomaMiddle Agedmedicine.diseasePrognosis1310 EndocrinologyPancreatic Neoplasms2712 Endocrinology Diabetes and MetabolismNeuroendocrine Tumorsmedicine.anatomical_structureSomatostatinOncologyDuodenum570 Life sciences; biology2730 OncologyFemalebusinessPancreasSomatostatinFollow-Up Studies
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Cytogenetic findings in secondary acute nonlymphocytic leukemia

1992

Abstract We here report the results of cytogenetic studies carried out in eight patients with acute nonlymphocytic leukemia developed after primary neoplasias. In seven of the reported cases, clonal chromosome aberrations were found, some being specific of de novo acute nonlymphocytic leukemia (ANLL). Numerical abnormalities were detected, such as the total monosomy of chromosomes 5, 7, 21, trisomy of chromosomes 8, 11, 15, and duplication of chromosome Y. Structural changes were also observed: a del(12)(p12), a del(16)(q22), the translocations t(3;5)(p21;q35),t(3;7)(p21;q35), and t(12;14)(p12;q32) and other changes involving chromosome 8. The finding of a hypertetraploid karyotype with com…

AdultMaleCancer Researchmedicine.medical_specialtyMonosomyChromosomal translocationBiologyTranslocation GeneticPolyploidyMonosomyhemic and lymphatic diseasesGeneticsmedicineHumansMolecular BiologyAgedChromosome AberrationsCytogeneticsChromosomeNeoplasms Second PrimaryKaryotypeMiddle Agedmedicine.diseaseLymphomaLeukemia Myeloid AcuteLeukemiaImmunologyCancer researchChromosomes Human Pair 5FemaleTrisomyChromosomes Human Pair 7Cancer Genetics and Cytogenetics
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Chromosomal abnormalities in Waldenström's macroglobulinemia

1992

We report the results of cytogenetic studies of direct bone marrow (BM) preparations and of short-term BM and peripheral blood (PB) cultures from 17 patients with Waldenström's macroglobulinemia. We noted clonal chromosome changes in 10 patients. Abnormalities affected chromosomes X, Y, 2, 4, 5, 15, 16, 18, 19, 20, 21, and 22; in particular, chromosomes 2, 4, and 5 were involved in structural changes: a homogeneously staining region [hsr(2)], a der(4)t(4;?)(q32;?), and a 5q+. The other chromosomes were involved in numerical abnormalities, such as pseudodiploidy (a 46,X,-X,+15 clone), loss of chromosome Y, and monosomy of chromosomes 16, 18, 19, 20, 21, and 22. Nonclonal chromosome rearrange…

AdultMaleCancer Researchmedicine.medical_specialtyMonosomyClone (cell biology)Chromosome rearrangements Waldenström's macroglobulinemiaBiologyGeneticsmedicineHumanseducationMolecular BiologyHomogeneously Staining RegionAgedGeneticsAged 80 and overChromosome Aberrationseducation.field_of_studyCytogeneticsMacroglobulinemiaWaldenstrom macroglobulinemiaChromosomeKaryotypeMiddle Agedmedicine.diseaseMolecular biologySettore BIO/18 - GeneticaChromosomes Human Pair 2FemaleWaldenstrom Macroglobulinemia
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Treatment of 11 patients with chronic myelogenous leukemia with interferon-alpha-2C and low-dose cytosine arabinoside

1993

Abstract Patients with Philadelphia (Ph) chromosome-positive chronic myelogenous leukemia (CML) and on interferon (IFN)-α-2c treatment for at least two months were entered in the present pilot study. IFN-α treatment was maintained identically and cytosine arabinoside (Ara-C) was added at monthly cycles of 10 mg/m 2 /day for ten days subcutaneously. In the case of a leukocyte nadir above 10 G/1, the Ara-C dose was increased to 20 mg/m 2 /day for 10 days per month. Ten of the eleven patients entered in this study were evaluable for toxicity and response. They received a total of 87 IFN-α/Ara-C cycles (3–14/patient). Five patients received 1–5 cycles with Ara-C dose intensification to 20 mg/m …

AdultMaleCancer Researchmedicine.medical_specialtyNauseamedicine.medical_treatmentAlpha interferonGastroenterologyDrug Administration ScheduleLeukocyte CountLeukemia Myelogenous Chronic BCR-ABL PositiveInternal medicinemedicineHumansInterferon alfaAgedChemotherapybusiness.industryCytarabineHematologyMiddle Agedmedicine.diseaseCombined Modality TherapyRecombinant ProteinsSurgeryDiarrheaOncologyInterferon Type IToxicityCytarabineFemalemedicine.symptombusinessmedicine.drugChronic myelogenous leukemiaLeukemia Research
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Salivary flow and xerostomia in patients with type 2 diabetes

2018

Background: Saliva is secreted by the major and minor salivary glands. There are a number of physiological factors that can reduce this secretion such as age, sex, body weight, number of teeth present in the mouth or time of day. This decrease may also be caused by the use of certain drugs, radiotherapy for head and neck cancer, chronic rheumatic diseases such as Sjögren's syndrome and other systemic disorders such as diabetes mellitus (DM). Objective of this study was to investigate the effect of type 2 DM on salivary secretion and its relation to the sensation of xerostomia. Methods: Forty-seven patients with type 2 DM and 46 healthy individuals, aged 40-80, participated in the study. Sam…

AdultMaleCancer Researchmedicine.medical_specialtySalivadiabetes mellitus type 2saliva xerostomia diabetes mellitus type 22734030209 endocrinology & metabolismStimulationType 2 diabetesSalivary GlandsPathology and Forensic MedicineNO03 medical and health sciencessymbols.namesake0302 clinical medicinestomatognathic systemSurveys and QuestionnairesInternal medicineDiabetes mellitusSensationmedicineHumansxerostomiaFisher's exact testAgedAged 80 and overMealsalivabusiness.industryOtorhinolaryngology2734 Pathology and Forensic MedicineHead and neck cancerMED/28 - MALATTIE ODONTOSTOMATOLOGICHE030206 dentistryMiddle Agedmedicine.diseasestomatognathic diseasesDiabetes Mellitus Type 2OtorhinolaryngologysymbolsPeriodonticsFemaleOral Surgerybusiness
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Association Between Individual and Combined SNPs in Genes Related to Innate Immunity and Incidence of CMV Infection in Seropositive Kidney Transplant…

2014

In this study, we assessed the association between single-nucleotide polymorphisms (SNPs) in seven candidate genes involved in orchestrating the immune response against cytomegalovirus (CMV) and the 12-month incidence of CMV infection in 315 CMV-seropositive kidney transplant (KT) recipients. Patients were managed either by antiviral prophylaxis or preemptive therapy. CMV infection occurred in 140 patients (44.4%), including 13 episodes of disease. After adjusting for various clinical covariates, patients harboring T-allele genotypes of interleukin-28B (IL28B) (rs12979860) SNP had lower incidence of CMV infection (adjusted hazard ratio [aHR]: 0.66; 95% confidence interval [CI]: 0.46-0.96; p…

AdultMaleCandidate geneGenotypeReceptors Cell SurfaceSingle-nucleotide polymorphismDiseasePolymorphism Single NucleotideImmune systemGenotypeOdds RatioHumansImmunology and AllergyMedicineSNPLectins C-TypePharmacology (medical)Prospective StudiesAllelesAgedProportional Hazards ModelsTransplantationbusiness.industryIncidenceInterleukinsHazard ratioTLR9Middle AgedKidney TransplantationImmunity InnateTransplant RecipientsCytomegalovirus InfectionsImmunologyKidney Failure ChronicFemaleInterferonsbusinessCell Adhesion MoleculesAmerican Journal of Transplantation
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Large-Scale Gene-Centric Meta-Analysis across 39 Studies Identifies Type 2 Diabetes Loci

2012

To identify genetic factors contributing to type 2 diabetes (T2D), we performed large-scale meta-analyses by using a custom ∼50,000 SNP genotyping array (the ITMAT-Broad-CARe array) with ∼2000 candidate genes in 39 multiethnic population-based studies, case-control studies, and clinical trials totaling 17,418 cases and 70,298 controls. First, meta-analysis of 25 studies comprising 14,073 cases and 57,489 controls of European descent confirmed eight established T2D loci at genome-wide significance. In silico follow-up analysis of putative association signals found in independent genome-wide association studies (including 8,130 cases and 38,987 controls) performed by the DIAGRAM consortium id…

AdultMaleCandidate geneSNP ARRAYAdolescentGenotypeSUSCEPTIBILITY LOCI030209 endocrinology & metabolismGenome-wide association studySingle-nucleotide polymorphismLocus (genetics)BLOOD-PRESSUREBiologyPolymorphism Single NucleotideArticleYoung Adult03 medical and health sciences0302 clinical medicineEthnicityGeneticsHumansEUROPEAN AMERICANSGenetic Predisposition to DiseaseRESOURCE CAREGenetics(clinical)GENOME-WIDE ASSOCIATIONGenetics (clinical)Aged030304 developmental biologyGenetic associationAged 80 and overGeneticsAFRICAN-AMERICANS0303 health sciencesINSULIN-RESISTANCECOMMON VARIANTSMiddle Aged3. Good healthSNP genotypingDiabetes Mellitus Type 2Genetic LociCase-Control StudiesRISK-FACTORSFemaleTCF7L2Follow-Up StudiesGenome-Wide Association StudySNP array
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klebsiella pneumoniae-reactive t cells in blood and synovial fluid of patients with ankylosing spondylitis comparison with hla–b27 + healthy control …

1995

Objective To study the frequency of Klebsiella pneumoniae-responsive T cells in the peripheral blood (PB) of ankylosing spondylitis (AS) patients compared with that in healthy HLA-B27+ donors, and to examine T lymphocyte clones (TLC) derived from AS patient synovial fluid (SF) for the presence of Klebsiella reactivity. Methods Limiting dilution analysis of PB T cells in 8 patients with active AS and in 8 HLA-B27+ healthy subjects was used to determine the frequency of PB T cells responsive to K pneumoniae and Escherichia coli GroEL. SF T cells from a patient with active AS were cloned, and 125 TLC were characterized in proliferation assays. Results There were fewer T cells in the PB of AS p…

AdultMaleCellular immunityT-LymphocytesT cellImmunologyEpitopeEpitopesImmune systemRheumatologyReference ValuesSynovial FluidMethodsmedicineHumansImmunology and AllergySynovial fluidSpondylitis AnkylosingPharmacology (medical)HLA-B27 AntigenAgedHLA-B27Blood Cellsbusiness.industryChaperonin 60T lymphocyteMiddle AgedMolecular biologyClone CellsKlebsiella pneumoniaePhenotypemedicine.anatomical_structureImmunologyFemaleBacterial antigenbusinessArthritis & Rheumatism
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Oligophrenin 1 mutations frequently cause X-linked mental retardation with cerebellar hypoplasia

2005

Background: Mutations of oligophrenin 1, one of the first genes identified in nonspecific X-linked mental retardation (MRX), have been described in patients with moderate to severe cognitive impairment and predominant cerebellar hypoplasia, in the vermis. Objective: To further delineate the phenotypic and mutational spectrum of the syndrome, by screening oligophrenin 1 in two cohorts of male patients with mental retardation (MR) with or without known posterior fossa anomalies. Methods: Clinical examination, cognitive testing, MRI studies, and mutational analysis (denaturing gradient gel electrophoresis and direct sequencing) on blood lymphocytes were performed in 213 unrelated affected indi…

AdultMaleCerebellumAdolescentGenotypeDNA Mutational AnalysisNonsense mutationNervous System Malformationsmedicine.disease_causeCohort StudiesExonCerebellar DiseasesCerebellummedicineHumansGenetic TestingChildCerebellar hypoplasiaGeneticsMutationSplice site mutationGTPase-Activating ProteinsNuclear Proteinsmedicine.diseaseMagnetic Resonance ImagingHypoplasiaPedigreeDevelopmental disorderAlternative SplicingCytoskeletal ProteinsPhenotypemedicine.anatomical_structureFacial AsymmetryCodon NonsenseChild PreschoolMutationMental Retardation X-LinkedRNA Splice SitesNeurology (clinical)PsychologyGene DeletionNeurology
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