Search results for "units"

showing 10 items of 576 documents

Prevalence survey of healthcare-associated infections and antimicrobial use at the university hospital "paolo giaccone", palermo, italy

2013

Introduction. Healthcare-associated infections (HAIs) and antimicrobial resistance are well known major public health threats. The first goal of our study was to describe the prevalence of HAI, while the second goal was to describe the antibiotic consumption at our University Hospital, ?P. Giaccone? in Palermo, Italy. Methods. A standardized methodology for a combined Point Prevalence Survey (PPS) on healthcare-associated infections (HAIs) and antimicrobial use in European acute care hospital developed by the European Centre for Disease Prevention and Control (ECDC) was piloted across Europe. The teaching Hospital ?P. Giaccone? in Palermo, Italy, participated in the study Results. Out of 32…

MaleAntimicrobials drug resistanceCross Infectionvirus diseasesBacteremiaHealthcare-associated infectionsAntibiotic ProphylaxisMiddle AgedSettore MED/42 - Igiene Generale E ApplicataDrug UtilizationAnti-Bacterial AgentsHospitals UniversityIntensive Care UnitsItalyPoint Prevalence SurveyDrug Resistance BacterialPrevalenceHumansOriginal ArticleFemaleHealthcare associated infection Antibiotic Prophylaxis Prevalence Hospital University
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De novo loss-of-function KCNMA1 variants are associated with a new multiple malformation syndrome and a broad spectrum of developmental and neurologi…

2019

Abstract KCNMA1 encodes the large-conductance Ca2+- and voltage-activated K+ (BK) potassium channel α-subunit, and pathogenic gain-of-function variants in this gene have been associated with a dominant form of generalized epilepsy and paroxysmal dyskinesia. Here, we genetically and functionally characterize eight novel loss-of-function (LoF) variants of KCNMA1. Genome or exome sequencing and the participation in the international Matchmaker Exchange effort allowed for the identification of novel KCNMA1 variants. Patch clamping was used to assess functionality of mutant BK channels. The KCNMA1 variants p.(Ser351Tyr), p.(Gly356Arg), p.(Gly375Arg), p.(Asn449fs) and p.(Ile663Val) abolished the …

MaleAtaxiaGenotypeDevelopmental DisabilitiesMutation MissenseBiology03 medical and health sciences0302 clinical medicineNeurodevelopmental disorderProtein DomainsLoss of Function MutationGeneticsmedicineHumansMissense mutationAbnormalities MultipleGenetic Predisposition to DiseaseProtein Interaction Domains and MotifsAlleleLarge-Conductance Calcium-Activated Potassium Channel alpha SubunitsMolecular BiologyAllelesGenetic Association StudiesGenetics (clinical)Loss functionExome sequencing030304 developmental biologyGenetics0303 health sciencesInfant NewbornGeneral MedicineParoxysmal dyskinesiamedicine.diseaseElectrophysiological PhenomenaPedigreePhenotypeAmino Acid SubstitutionSpeech delayFemaleGeneral Articlemedicine.symptom030217 neurology & neurosurgeryHuman Molecular Genetics
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Association of a functional deficit of the BKCa channel, a synaptic regulator of neuronal excitability, with autism and mental retardation

2006

International audience; Objective: Autism is a complex, largely genetic psychiatric disorder. In the majority of cases, the cause of autism is not known, but there is strong evidence for a genetic etiology. To identify candidate genes, the physical mapping of balanced chromosomal aberrations is a powerful strategy, since several genes have been characterized in numerous disorders. In this study, the authors analyzed a balanced reciprocal translocation arising de novo in a subject with autism and mental retardation. Method: The authors performed the physical mapping of the balanced 9q23/ 10q22 translocation by fluorescent in situ hybridization experiments using bacterial artificial chromosom…

MaleCandidate geneChromosomes Artificial BacterialIndolesDNA Mutational AnalysisRegulatorChromosomal translocationautism mental retardation KCNMA1 genelarge conductance Ca(2+)-activated K(+) (BK(Ca)) channel synaptic transmission chromosomal translocationSynaptic TransmissionTranslocation GeneticPair 10CA2+-ACTIVATED K+ CHANNELSCloning MolecularChildLarge-Conductance Calcium-Activated Potassium Channel alpha SubunitsMUTATIONIn Situ HybridizationIn Situ Hybridization FluorescenceReverse Transcriptase Polymerase Chain ReactionBacterialChromosome MappingETIOLOGYPsychiatry and Mental healthArtificialKCNMA1 Gene[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]HaploinsufficiencyPsychologyChromosomes Human Pair 9POTASSIUM CHANNELSHumanPair 9Autistic Disorder; Child; Chromosome Aberrations; Chromosome Mapping; Chromosomes; Artificial; Bacterial; Chromosomes; Human; Pair 10; Chromosomes; Human; Pair 9; Cloning; Molecular; DNA Mutational Analysis; Humans; In Situ Hybridization; Fluorescence; Indoles; Intellectual Disability; Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; Male; Reverse Transcriptase Polymerase Chain Reaction; Synaptic Transmission; Translocation; GeneticTranslocationNeurotransmissionChromosomesFluorescenceGeneticIntellectual DisabilitymedicineHumansAutistic DisorderRELEASEChromosome AberrationsCOMPLEXChromosomes Human Pair 10MolecularAutistic Disorder; Child; Chromosome Aberrations; Chromosome Mapping; Chromosomes Artificial Bacterial; Chromosomes Human Pair 10; Chromosomes Human Pair 9; Cloning Molecular; DNA Mutational Analysis; Humans; In Situ Hybridization Fluorescence; Indoles; Intellectual Disability; Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; Male; Reverse Transcriptase Polymerase Chain Reaction; Synaptic Transmission; Translocation GeneticPERVASIVE DEVELOPMENTAL DISORDERSmedicine.diseaseDevelopmental disorderINDIVIDUALSLARGE-CONDUCTANCEAutismSCREENNeuroscience[SDV.MHEP]Life Sciences [q-bio]/Human health and pathologyCloning
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Characterization and referral patterns of ST-elevation myocardial infarction patients admitted to chest pain units rather than directly to catherizat…

2017

Abstract Background Direct transfer to the catheterization laboratory for primary percutaneous coronary intervention (PCI) is standard of care for patients with ST-segment elevation myocardial infarction (STEMI). Nevertheless, a significant number of STEMI-patients are initially treated in chest pain units (CPUs) of admitting hospitals. Thus, it is important to characterize these patients and to define why an important deviation from recommended clinical pathways occurs and in particular to quantify the impact of deviation on critical time intervals. Methods and results 1679 STEMI patients admitted to a CPU in the period from 2010 to 2015 were enrolled in the German CPU registry (8.5% of 19…

MaleCardiac CatheterizationChest Painmedicine.medical_specialtyReferralmedicine.medical_treatmentMedizin030204 cardiovascular system & hematologyDirect transferCoronary AngiographyChest painLower riskTime-to-TreatmentElectrocardiography03 medical and health sciences0302 clinical medicineSt elevation myocardial infarctionGermanymedicineHumansRegistriescardiovascular diseases030212 general & internal medicineMyocardial infarctionReferral and Consultationbusiness.industryIncidenceCoronary Care UnitsPercutaneous coronary interventionMiddle AgedLaboratories Hospitalmedicine.diseaseSurvival RateEmergency medicineConventional PCIST Elevation Myocardial InfarctionFemalemedicine.symptomCardiology and Cardiovascular Medicinebusiness
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Growth Differentiation Factor-15 (GDF-15) Levels Are Associated with Cardiac and Renal Injury in Patients Undergoing Coronary Artery Bypass Grafting …

2014

International audience; Objective: Growth differentiation factor-15 (GDF-15) has been identified as a strong marker of cardiovascular disease; however, no data are available concerning the role of GDF-15 in the occurrence of organ dysfunction during coronary artery bypass grafting (CABG) associated with cardiopulmonary bypass (CPB). Methods: Five arterial blood samples were taken sequentially in 34 patients from anesthesia induction (IND) until 24 h after arrival at the intensive care unit (ICU). Plasma levels of GDF-15, follistatin-like 1 (FLST1), myeloperoxidases (MPO), hydroperoxides and plasma antioxidant status (PAS) were measured at each time-point. Markers of cardiac (cardiac-troponi…

MaleCardiothoracic SurgeryCardiovascular ProceduresGeneral Anesthesialcsh:Medicine030204 cardiovascular system & hematologyAntioxidantslaw.inventionPostoperative Complications0302 clinical medicineAnesthesiologylawTroponin IMedicine and Health SciencesCoronary Heart DiseaseMedicineAnesthesiaCoronary Artery Bypasslcsh:Science0303 health sciencesCardiopulmonary BypassCoronary Artery Bypass GraftingMultidisciplinaryAcute kidney injuryAcute Kidney InjuryMiddle AgedLipocalins[SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system3. Good healthCardiac surgeryIntensive Care UnitsCardiovascular Diseasesembryonic structuresCardiologyArterial bloodFemalemedicine.symptomResearch Articlemedicine.medical_specialtyFollistatin-Related ProteinsGrowth Differentiation Factor 15Heart DiseasesCardiac SurgeryCardiologyRenal functionSurgical and Invasive Medical Procedures03 medical and health sciencesLipocalin-2[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular systemPredictive Value of TestsProto-Oncogene ProteinsInternal medicineCardiopulmonary bypassHumansAgedPeroxidase030304 developmental biologybusiness.industryTroponin Ilcsh:ROrgan dysfunctionHydrogen Peroxidemedicine.diseaseSurgerylcsh:QGDF15businessBiomarkersAcute-Phase Proteins
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The German CPU Registry: Comparison of troponin positive to troponin negative patients

2013

Lars S. Maier ⁎, Harald Darius , Evangelos Giannitsis , Raimund Erbel , Michael Haude , Christian Hamm , Gerd Hasenfuss , Gerd Heusch , Harald Mudra , Thomas Munzel , Claus Schmitt , Burghard Schumacher , Jochen Senges , Thomas Voigtlander , Jan B. Schuttert a a Dept. of Cardiology & Pneumology/Heart Centre, Georg-August-University, Gottingen, Germany b Dept. of Cardiology, Angiology & Intensive Care Medicine, Vivantes Hospital Neukolln, Berlin, Germany c Dept. of Cardiology, Angiology & Pneumology, Ruprecht-Karls-University, Heidelberg, Germany d Dept. of Cardiology, West-German Heart Centre, University of Essen, Germany e Dept. of Cardiology & Nephrology, Lukas Hospital, Neuss, Germany f …

MaleChest Painmedicine.medical_specialtyMedizin030204 cardiovascular system & hematologyGerman03 medical and health sciences0302 clinical medicineGermanyHumansMedicineProspective StudiesRegistries030212 general & internal medicineQuality of careChest pain units; Myocardial infarction; Quality of care; TroponinAgedAngiologyChest pain unitsbusiness.industryTroponin IQuality of careMiddle Agedmedicine.diseaseTroponinlanguage.human_language3. Good healthMyocardial infarctionlanguageFemaleMedical emergencyCardiology and Cardiovascular MedicinebusinessFollow-Up StudiesInternational Journal of Cardiology
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Laboratory investigations in genetic syndromes: examples of clinical approach in the neonatal unit.

2010

Contiguous gene deletion syndromes: the importance of an accurate genetic definition for a careful clinical monitoring. Contiguous gene deletion syndromes are so named because the deletion manifests as a distinctive cluster of otherwise unrelated single-gene disorders in the same subject. An accurate genetic definition of the deleted region is extremely important for the appropriate management of these patients and for unravelling the function of the involved genes. The microarray-based comparative genomic hybridization (CGH arrays) analysis is the actual molecular method able to accurately define the bounds of a deleted region, since it allows an evaluation of DNA copy number alterations a…

MaleComparative Genomic Hybridizationgenetic syndrome neonategenetic syndromesDisorders of Sex DevelopmentGene DosageGenetic Diseases InbornInfant NewbornLaboratories HospitalEarly DiagnosisPhenotypeSettore MED/38 - Pediatria Generale E SpecialisticaIntensive Care Units NeonatalIntensive Care NeonatalChromosomes HumanHumansFemaleGenetic TestingGene Deletion
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Psychological experience of patients 3 months after a stay in the intensive care unit: A descriptive and qualitative study.

2015

Abstract Purpose The purpose was to describe psychological experiences of patients 3 months after a stay in the intensive care unit (ICU) using qualitative methods. Methods Twenty patients underwent clinical interview lasting 1 hour and completed the Impact of Event Scale—Revised and Hospital Anxiety and Depression questionnaires. All interviews were recorded and coded using thematic analysis. Results All patients (100%) reported that they could not remember their ICU stay; half reported confused memories (50%) or disorientation (50%). Negatives memories were also reported (20%-45%), namely, pain, distress, sleep difficulties, noise, fear, feeling of abandonment; 20% reported positive memor…

MaleCoping (psychology)medicine.medical_specialtymedia_common.quotation_subjectFamily supportEmotions[SHS.PSY]Humanities and Social Sciences/PsychologyAnxietyCritical Care and Intensive Care Medicinelaw.inventionStress Disorders Post-Traumatic03 medical and health sciences0302 clinical medicineOptimismlawSurveys and QuestionnairesAdaptation PsychologicalInterview PsychologicalmedicineHumans030212 general & internal medicineProspective StudiesPsychiatryComputingMilieux_MISCELLANEOUSQualitative Researchmedia_commonAgedbusiness.industryDepression030208 emergency & critical care medicineFearLength of StayMiddle AgedIntensive care unitPatient Discharge3. Good healthDistressIntensive Care UnitsMemory Short-TermFeelingAnxietyFemaleThematic analysismedicine.symptombusinessJournal of critical care
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Guideline-adherence regarding critical time intervals in the German Chest Pain Unit registry

2020

Background: Since 2008, the German Cardiac Society certified 256 Chest Pain Units (CPUs). Little is known about adherence to recommended performance measures in patients with suspected acute coronary syndrome (ACS) presenting to CPUs. We investigated guideline-adherence regarding critical time intervals and selected performance measures in German Chest Pain Units. Methods: From 2008 to 2014, 23,804 consecutive patients with suspected ACS were prospectively enrolled in the Chest Pain Unit registry of the German Cardiac Society. Results: Median time from symptom onset to first medical contact was 2 h in patients with ST-elevation myocardial infarction (STEMI) and 4 h in patients with unstable…

MaleCritical timeMedizinische Fakultät » Universitätsklinikum Essen » Institut für PathophysiologieTime FactorsMedizin030204 cardiovascular system & hematologyCritical Care and Intensive Care MedicineChest painGermanElectrocardiography610 Medical sciences Medicine0302 clinical medicineGermanyProspective StudiesRegistries030212 general & internal medicineNon-ST Elevated Myocardial InfarctionGuideline adherenceGeneral MedicineMiddle AgedHospitalizationlanguageFemaleAcute coronary syndromeGuideline Adherencemedicine.symptomCardiology and Cardiovascular MedicineHospital UnitsChest Painmedicine.medical_specialtyAcute coronary syndromeguideline-adherence61003 medical and health sciencesPercutaneous Coronary Interventiontime intervalsmedicineHumansIn patientddc:610Angina Unstablecardiovascular diseasesAcute Coronary SyndromeAgedbusiness.industryUnstable anginamedicine.diseaseChest Pain Unitlanguage.human_languageEmergency medicineExercise TestST Elevation Myocardial InfarctionTomography X-Ray ComputedbusinessEuropean Heart Journal: Acute Cardiovascular Care
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A discoidal lipoprotein from the coelomic fluid of the polychaete Nereis virens.

2005

A discoidal lipoprotein was isolated from the coelomic fluid of the polychaete, Nereis virens, by density gradient centrifugation. The lipoprotein was present in both sexes and moved as a uniform band in an agarose gel. The average diameter of the lipoprotein particles determined by electron microscopy was 42 nm with a thickness of 10 nm. SDS electrophoresis showed two apoprotein subunits with molecular masses of 247 and 85 kDa, respectively. In lectin blots, both apoproteins were reactive with Concanavalin A indicating the presence of N-glycans. The small subunit was also reactive with peanut lectin, indicating additional O-glycosylation. The total lipid content was 48% and consisted mainl…

MaleEmbryo NonmammalianGlycosylationPhysiologyLipoproteinsBiologyIn Vitro TechniquesBiochemistrychemistry.chemical_compoundPolysaccharidesAnimalsParticle SizeMolecular BiologyDifferential centrifugationMolecular massLectinPolychaetaLipidsThin-layer chromatographyBody FluidsMolecular WeightElectrophoresisProtein SubunitschemistryBiochemistryConcanavalin Abiology.proteinAgaroselipids (amino acids peptides and proteins)FemaleLipoproteinComparative biochemistry and physiology. Part B, Biochemistrymolecular biology
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