Search results for "valuation"
showing 10 items of 2494 documents
Voltage gradient electrophoresis of nucleic acids on agarose gels.
1993
A very simple method is described which allows the separation of DNA molecules in a wide molecular weight range (from 0.6 to about 30 kb) in the same electrophoresis agarose gel. This is based on the achievement of a voltage gradient through a simple device consisting of a Plexiglas plate placed slantwise with respect to the gel surface plane, submerged in the electrophoretic running buffer. Further applications of our system are also described.
Quantitative RT-PCR measurement of human cytochrome P-450s: application to drug induction studies.
2000
A quantitative RT-PCR assay has been developed that is able to measure the mRNA content of the major human CYPs (1A1, 1A2, 2A6, 2B6, 2C9, 2C19, 2D6, 2E1, 3A4, and 3A5). The technique is highly specific, reproducible, rapid, and sensitive enough to quantitate low and high abundant mRNAs. The PCR primers were selected to specifically match each CYP mRNA, to have a very close annealing temperature, and to render PCR products of similar sizes. The PCR conditions were designed to allow the simultaneous measurement of the various human liver CYPs in a single run. To achieve precise and reproducible quantitation of each cytochrome mRNA, a external standard (luciferase mRNA) is added to the probes …
Semi-automatic quantitative RT-PCR to measure CYP induction by drugs in human hepatocytes
2003
An assay has been developed for the quantitative measurement of CYP mRNA content of the major human isoforms (1A1, 1A2, 2A6, 2B6, 2C9, 2C19, 2D6, 2E1, 3A4 and 3A5) in human hepatocytes. The method is based on the conversion of mRNAs into their corresponding cDNAs, followed by PCR amplification using appropriate primers. Making use of appropriate internal and external standards it is possible to estimate changes in CYP mRNA content of hepatocytes. The technique has been standardised to run semi-automatically. This procedure can be used to assess the CYP induction potential of new pharmaceuticals at a pre-clinical stage of development. To this aim, human hepatocytes obtained from functional l…
The Role of Assumptions in Ohlson Model Performance: Lessons for Improving Equity-Value Modeling
2021
In this paper, we test whether the short-run econometric conditions for the basic assumptions of the Ohlson valuation model hold, and then we relate these results with the fulfillment of the short-run econometric conditions for this model to be effective. Better future modeling motivated us to analyze to what extent the assumptions involved in this seminal model are not good enough approximations to solve the firm valuation problem, causing poor model performance. The model is based on the well-known dividend discount model and the residual income valuation model, and it adds a linear information model, which is a time series model by nature. Therefore, we adopt the time series approach. In…
SOLUZIONI OPERATIVE ED ASPETTI QUALITATIVI PER LA GEOCODIFICA DI POPOLAZIONI IN AMBITO SANITARIO.
2015
IntroduzioneL'utilizzo dei Geographic Information Systems (GIS) in ambito sanitario, continua a ricevere grande attenzione. La disponibilità di informazioni sulla localizzazione degli individui, consente di effettuare associazioni territoriali sulle cause delle patologie, nonché di indagare aspetti legati alla distribuzione dei servizi sanitari ed all'equità di accesso alle cure. ObiettiviAnalizzare le principali problematiche e le soluzioni operative per la geocodifica di popolazioni e proporre un indice di qualità della geocodifica di cui poter tenere conto in sede di analisi. MetodiCome caso studio vengono illustrate le fasi che hanno portato alla georeferenziazione di indirizzi residenz…
School Neighbourhood Built Environment Assessment for Adolescents’ Active Transport to School: Modification of an Environmental Audit Tool and Protoc…
2020
School neighbourhood built environments (SN-BE) can influence adolescents&rsquo
Geobotanical approach to detect land-use change of a Mediterranean landscape: a case study in Central-Western Sicily
2018
A landscape is a palimpsest of the interactions between human activities and ecological dynamics. In an interdisciplinary perspective of dialogue between the ‘Two Cultures’ (Natural Sciences and Humanities), a study of a rural area has been carried out through a reading of plant ecosystems as signs of human impact. The purpose of this paper—as part of the project ‘Harvesting Memories’: Ecology and landscape archaeology of Castro/Giardinallo Valley and Mt. Barrau district (Corleone, Palermo, Sicily)—is to analyse the formative-processes of a Sicilian rural landscape and its changes in the last century. A key element in the reconstruction of the formation of the present landscape is the serie…
Social Insurance Accounting for a Notional Defined Contribution Scheme Combining Retirement and Long-Term Care Benefits
2018
This paper develops a social insurance accounting model for a notional defined contribution (NDC) scheme combining retirement and long-term care (LTC) contingencies. The procedure relies on standard double-entry bookkeeping and enables us to compile a “Swedish” type actuarial balance sheet (ABS) following a framework equivalent to an open group approach. This methodology is suitable for reporting the system’s solvency status and can show periodical changes in the system’s financial position by means of an income statement. The information underpinning the actuarial valuation is based on events and transactions that are verifiable at the valuation date, without consid…
Real Options: an Application to RMS Investment Evaluation
2007
LKS-92 Coordinates Transformation to ITRF2000
2014
LKS-92 is Latvian geodetic coordinate system used as an EUREF89 realization in Latvia. LKS-92 is official coordinate system for procuring of essential geospatial data. For aeronautical and other purposes ITRF2000 is used in publication of geospatial data. Research is done to obtain transformation parameters from LKS-92 realization epoch 1992.75 through LatPos to ITRF2000 epoch 2000.00. Valuation of two different coordinate adjustment strategies and accuracy of parameters is done. Results of research could be used for transformation from LKS-92 to ITRF2000 for all kind of geospatial data.