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showing 10 items of 14701 documents
Cryptic diversity within three South American whip spider species (Arachnida, Amblypygi)
2020
4 pages; International audience; Cryptic diversity (CD), the presence of highly divergent phylogenetic lineages within closed morphological species, has been documented for many taxa. Great arachnid orders such as Araneae or Scorpiones are well studied and many cases of CD have been described therein; to date, however, related research on smaller arachnid orders, such as whip spiders (Amblypygi), remains lacking. In the current study, we investigated CD based on cytochrome oxidase 1 (COI) in three nominal species of the genus Heterophrynus (H. alces, H. batesii, and H. longicornis), represented by 65 specimens. The sequences were compared using three different methods. All three species sho…
Give me a sample of air and I will tell which species are found from your region : Molecular identification of fungi from airborne spore samples
2017
Fungi are a megadiverse group of organisms, they play major roles in ecosystem functioning and are important for human health, food production and nature conservation. Our knowledge on fungal diversity and fungal ecology is however still very limited, in part because surveying and identifying fungi is time demanding and requires expert knowledge. We present a method that allows anyone to generate a list of fungal species likely to occur in a region of interest, with minimal effort and without requiring taxonomical expertise. The method consists of using a cyclone sampler to acquire fungal spores directly from the air to an Eppendorf tube, and applying DNA barcoding with probabilistic specie…
Micropropagation of Sicilian cultivars with an aim to preserve genetic diversity in hazelnut (Corylus avellana L.)
2018
The use of a small number of cultivars in agriculture can lead to a loss of agrobiodiversity. Since in vitro techniques are valuable tools for conserving plant biodiversity, an efficient micropropagation protocol for four Italian hazelnut cultivars, ‘Carrello’, ‘Ghirara’, ‘Minnulara’, and ‘Panottara’, was developed. The highest axillary bud survival was obtained after decontamination with 40 min 1% sodium hypochlorite followed by 40 min 0.1% sodium merthiolate in ‘Minnulara’ and ‘Ghirara’, while the 35þ35 min treatment was the best for ‘Carrello’ and ‘Panottara’. Shoot multiplication was higher in ‘Minnulara’ and ‘Ghirara’ when 6.6 lM N6-benzyladenine was used, even if some hyperhydric shoo…
Toward the valorization of olive (Olea europaea var. europaea L.) biodiversity: horticultural performance of seven Sicilian cultivars in a hedgerow p…
2019
Abstract An intense survey of the Sicilian’s olive growing areas for autochthonous germplasm, mainly represented by centennials olive trees (Olea europaea var. europaea L.) apparently older then III centuries, started at the beginning of the 1980s and resulted in the selection of more than 150 cultivars and accessions. This germplasm was propagated in a nursery, by grafting onto seedlings of Olea europaea L., and planted in an experimental orchard, in an olive district located in the South-west of the Island, where they were evaluated for over 30 years and selected for their early bearing, high and constant productivity, as well as high oil content of the fruits and excellent chemical (olei…
Microspore Embryogenesis Through Anther Culture in Citrus clementina Hort. ex Tan.
2016
Anther culture is a biotechnological method that allows to obtain, in one step, homozygous plants, very important to plant breeding, due to their numerous applications in mutation research, selection, genome sequencing, genetic analysis, and transformation. To induce the microspores, i.e., the immature male gametes, to switch from the normal gametophytic pathway to the sporophytic one, it is necessary to submit them to a type of stress, such as high or low temperature, starvation, or magnetic field. Stress can be applied to the donor plants and/or the fl oral buds or the anthers or the isolated microspores, before or during the culture. In this chapter, the protocol to induce gametic embryo…
Quantitative genetics of temperature performance curves of Neurospora crassa
2020
AbstractEarth’s temperature is increasing due to anthropogenic CO2emissions; and organisms need either to adapt to higher temperatures, migrate into colder areas, or face extinction. Temperature affects nearly all aspects of an organism’s physiology via its influence on metabolic rate and protein structure, therefore genetic adaptation to increased temperature may be much harder to achieve compared to other abiotic stresses. There is still much to be learned about the evolutionary potential for adaptation to higher temperatures, therefore we studied the quantitative genetics of growth rates in different temperatures that make up the thermal performance curve of the fungal model systemNeuros…
Seasonal changes in morpho-functional aspects of two Anemonia sulcata (Pennant, 1777) wild populations
2017
Marine benthic organisms can be used as indicators of the quality of environmental status and as monitoring tools to detect natural or anthropogenic perturbations. In temperate waters, metabolic and biochemical responses may be governed by physiological changes driven by seasonal factors. Gathering baseline information on the mechanisms underlying seasonal acclimation patterns is therefore a critical step towards the understanding of the physiological responses of biological indicators. In poikilothermic metazoans, the production of regulatory metabolic enzymes can be used as tools for deciphering the acclimation potential. The aim of this study was to characterize the natural seasonal vari…
Corncrake conservation genetics at a European scale: the impact of biogeographical and anthropological processes
2016
Abstract Understanding patterns of genetic structure, gene flow and diversity across a species range is required to determine the genetic status and viability of small peripheral populations. This is especially crucial in species distributed across a large range where spatial heterogeneity makes it difficult to predict the distribution of genetic diversity. Although biogeographical models provide expectations of how spatially structured genetic variation may be at the range scale, human disturbance may cause strong deviations from these theoretical predictions. In this study, we investigated genetic structure and demography at a pan-European scale in the corncrake Crex crex , a grassland bi…
Spatio-temporal dynamics of genetic variation in the Iberian lynx along its path to extinction reconstructed with ancient DNA
2017
here is the tendency to assume that endangered species have been both genetically and demographically healthier in the past, so that any genetic erosion observed today was caused by their recent decline. The Iberian lynx (Lynx pardinus) suffered a dramatic and continuous decline during the 20th century, and now shows extremely low genome- and species-wide genetic diversity among other signs of genomic erosion. We analyze ancient (N = 10), historical (N = 245), and contemporary (N = 172) samples with microsatellite and mitogenome data to reconstruct the species' demography and investigate patterns of genetic variation across space and time. Iberian lynx populations transitioned from low but …
Inter- and intraspecific hypervariability in interstitial telomeric-like repeats (TTTAGGG)n in Anacyclus (Asteraceae).
2017
BACKGROUND AND AIMS: Interstitial telomeric repeat (ITR) sites, consisting of tandem repeats of telomeric motifs localized at intrachromosomal sites, have been reported in a few unrelated organisms including plants. However, the causes for the occurrence of ITRs outside of the chromosomal termini are not fully understood. One possible explanation are the chromosomal rearrangements involving telomeric sites, which could also affect the location of other structural genome elements, such as the 45S rDNA. Taking advantage of the high dynamism in 45S rDNA loci previously found in Anacyclus (Asteraceae, Anthemideae), the occurrence and patterns of variation of ITRs were explored in this genus wit…