Search results for "virus"

Single-cell analysis of population context advances RNAi screening at multiple levels

2012

Isogenic cells in culture show strong variability, which arises from dynamic adaptations to the microenvironment of individual cells. Here we study the influence of the cell population context, which determines a single cell's microenvironment, in image‐based RNAi screens. We developed a comprehensive computational approach that employs Bayesian and multivariate methods at the single‐cell level. We applied these methods to 45 RNA interference screens of various sizes, including 7 druggable genome and 2 genome‐wide screens, analysing 17 different mammalian virus infections and four related cell physiological processes. Analysing cell‐based screens at this depth reveals widespread RNAi‐induce…

Health Beliefs of Wearing Facemasks for Influenza A/H1N1 Prevention : A Qualitative Investigation of Hong Kong Older Adults

2019

Although vaccination is the first-line strategy controlling and preventing influenza A/H1N1 for older adults, personal protective measures, such as wearing facemasks, are also important preventive behaviors to reduce the risk of becoming infected with influenza A/H1N1 during a pandemic. In the current study, we aimed to explore the belief-related psychological factors of wearing facemasks for influenza A/H1N1 prevention in a sample of Hong Kong older adults. Community-dwelling Chinese adults (N = 137) aged between 65 and 80 years (Mage = 75.10; SDage = 6.49) participated in semistructured interviews. Data were analyzed using theoretical thematic analysis and identified themes were matched d…

Viroporins, Examples of the Two-Stage Membrane Protein Folding Model

2015

Viroporins are small, α-helical, hydrophobic virus encoded proteins, engineered to form homo-oligomeric hydrophilic pores in the host membrane. Viroporins participate in multiple steps of the viral life cycle, from entry to budding. As any other membrane protein, viroporins have to find the way to bury their hydrophobic regions into the lipid bilayer. Once within the membrane, the hydrophobic helices of viroporins interact with each other to form higher ordered structures required to correctly perform their porating activities. This two-step process resembles the two-stage model proposed for membrane protein folding by Engelman and Poppot. In this review we use the membrane protein folding …

Influenza vaccination among healthcare workers and absenteeism from work due to influenza-like illness in a teaching hospital in Palermo

2012

<p><strong>Background</strong>: Annual flu vaccination of healthcare workers (HCWs) is recommended worldwide as the best way to prevent influenza and to avoid its transmission. However, in several European Countries, vaccination rate among HCWs is still less than 25%. The aim of this study was to determine the HCW vaccination coverage during a three year period in a large University Hospital, identifying socio demographic and occupational variables involved in the decision to accept influenza vaccination. Moreover, for the 2007-2008 season, we also assessed the effectiveness of influenza vaccination in reducing influenza-related absenteeism.</p><p><strong&gt…

Asymmetric [N–I–N]+halonium complexes in solution?

2020

Assessment of the solution equilibria of [bis(pyridine)iodine(I)]+ complexes by ESI-MS and NMR reveals the preference of iodine(I) to form complexes with a more basic pyridine. Mixtures of symmetric [bis(pyridine)iodine(I)]+ complexes undergo statistical ligand exchange, with a predominant entropic driving force favoring asymmetric systems. The influence of ligand basicity, concentration, temperature, and ligand composition is evaluated. Our findings are expected to facilitate the investigations, and the supramolecular and synthetic applications of halonium ions’ halogen bonds. peerReviewed

Echovirus 1 infectious entry via novel multivesicular bodies

2011

Clustering-triggered endocytic pathway of α2β1 integrin

2012

Cellular factors promoting efficient baculovirus internalization and gene delivery into human cells

2013

Interactions of α2β1 integrin and its ligands, type I collagen and echovirus 1

2015

La emergencia viral como consecuencia de la interacción entre las variabilidades genéticas del virus y huésped

2015

La variabilidad genética en las poblaciones de huéspedes para la susceptibilidad al patógeno es esencial para la propagación de un virus emergente. Los modelos matemáticos predicen que la tasa de difusión del patógeno se frena a medida que aumenta la frecuencia y la diversidad de alelos de resistencia en la población huésped148,230. Sin embargo, las pruebas experimentales de esta hipótesis son escasas. Por ese motivo, este trabajo aborda la cuestión de cuánta heterogeneidad genética del huésped es necesaria para el cambio del destino evolutivo del virus. El modelo de patosistema experimental utilizado en este trabajo está compuesto por el Virus del grabado del tabaco (TEV), aislado At17b (T…