0000000000001873
AUTHOR
Dan Cojoc
Speckle based sensing device for fast detection of malaria
We propose a new technique for malaria detection. It is based upon extraction of correlation based statistics of speckle patterns generated while illuminating red blood cells with a laser and inspecting them under a microscope.
Toward fast malaria detection by secondary speckle sensing microscopy
Diagnosis of malaria must be rapid, accurate, simple to use, portable and low cost, as suggested by the World Health Organization (WHO). Despite recent efforts, the gold standard remains the light microscopy of a stained blood film. This method can detect low parasitemia and identify different species of Plasmodium. However, it is time consuming, it requires well trained microscopist and good instrumentation to minimize misinterpretation, thus the costs are considerable. Moreover, the equipment cannot be easily transported and installed. In this paper we propose a new technique named "secondary speckle sensing microscopy" ((SM)-M-3) based upon extraction of correlation based statistics of s…
Monitoring Human Neutrophil Differentiation by Digital Holographic Microscopy
We report on the usefulness of digital holographic microscopy (DHM) for the assessment of human neutrophil differentiation from myeloid cells. The cell and nuclear regions have been designated by image segmentation of the optical phase function, and the changes of the cell nucleus morphology in relation to the whole cell morphology have been examined during the process of granulocytic differentiation into mature neutrophils in PLB-985 cell line. Nucleus phase volume and circularity and the ratios between the nucleus and the cell projected area and volume provide a reliable set of parameters to characterize the maturation process. As control, cell differentiation has been monitored in parall…
Single-shot, dual-mode, water-immersion microscopy platform for biological applications
A single-shot water-immersion digital holographic microscope combined with broadband (white light) illumination mode is presented. This double imaging platform allows conventional incoherent visualization with phase holographic imaging of inspected samples. The holographic architecture is implemented at the image space (that is, after passing the microscope lens), thus reducing the sensitivity of the system to vibrations and/or thermal changes in comparison to regular interferometers. Because of the off-axis holographic recording principle, quantitative phase images of live biosamples can be recorded in a single camera snapshot at full-field geometry without any moving parts. And, the use o…
Shift and scale-invariant correlator using a radially stretched phase-only filter
A radial stretching of the phase only filter depending on the energy angular distribution of the target spectrum is used to perform shift and scale invariant pattern recognition. The complex conjugate of a basic size target Fourier transform and the cumulative energy angular distribution are calculated. Then the radially stretched filter providing the same energy contribution to the correlation peak independent on the target size is prepared and used in a conventional correlator, with spherical-wave illumination. The maximum scale ratio of recognizable objects equals 1:1.5. Computer simulations and experimental results, showing the performance of the filter are presented.
Full field of view super-resolution imaging based on two static gratings and white light illumination.
The usage of two static gratings for obtaining super-resolved imaging dates back to the work by Bachl and Lukosz in 1967. However, in their approach a severe reduction in the field of view was the necessary condition for improving the resolution. We present an approach based on two static gratings without sacrificing the field of view. The key idea for not paying with the field of view is to use white light illumination to average the ghost images obtained outside the region of interest since the positions of those images are wavelength dependent. Moreover, large magnification is achieved by using a commercial microscope objective instead of a test system with a unity magnification as prese…
Full field of view super-resolution imaging via two static masks
The usage of two static gratings for obtaining super resolved imaging dates back to the work by Bachl and Lukosz in 1967. However, in that approach, a severe reduction in the field of view was the necessary condition for improving the resolution. In this paper we present two approaches that are also based upon two static gratings but without the need to sacrifice in the field of view. The key idea for not paying with the field of view is performed in two ways: First, by using white light illumination that averages the ghost images obtained outside the region of interest since the positions of those images are wavelength dependent. Second, by using two random functions for the encoding and t…
Novel image processing approach to detect malaria
In this paper we present a novel image processing algorithm providing good preliminary capabilities for in vitro detection of malaria. The proposed concept is based upon analysis of the temporal variation of each pixel. Changes in dark pixels mean that inter cellular activity happened, indicating the presence of the malaria parasite inside the cell. Preliminary experimental results involving analysis of red blood cells being either healthy or infected with malaria parasites, validated the potential benefit of the proposed numerical approach. S.F. acknowledges the grant from Area Science Park of Trieste. J.G. acknowledges the support he got from project FIS2013-47548-P. D.M. and B.F. acknowl…
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Comparative between the white light imaging and the retrieved phase distribution from DHM
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2D wrapped phase distribution retrieved from DHM
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3D unwrapped phase distribution retrieved from DHM
2885655.mov
Comparative between the white light imaging and the retrieved phase distribution from DHM
2885654.mov
3D unwrapped phase distribution retrieved from DHM
2885653.mov
2D wrapped phase distribution retrieved from DHM