6533b836fe1ef96bd12a0903
RESEARCH PRODUCT
Single-shot, dual-mode, water-immersion microscopy platform for biological applications
José ÁNgel Picazo-buenoVicente MicóVincent TorreDan CojocFederico Isepponsubject
MicroscopeMaterials scienceHolographyHolographyImage processing02 engineering and technology01 natural sciencesSettore BIO/09 - Fisiologialaw.invention010309 opticsOpticslawAtomic and Molecular Physics0103 physical sciencesMicroscopyImmersionElectrical and Electronic EngineeringEngineering (miscellaneous)LightingMicroscopybusiness.industryEquipment Design021001 nanoscience & nanotechnologyInterference microscopyAtomic and Molecular Physics and OpticsLens (optics)InterferometryHolography | Computer generated holography | microscopy DHMCalibrationDigital holographic microscopyand Optics0210 nano-technologybusinessdescription
A single-shot water-immersion digital holographic microscope combined with broadband (white light) illumination mode is presented. This double imaging platform allows conventional incoherent visualization with phase holographic imaging of inspected samples. The holographic architecture is implemented at the image space (that is, after passing the microscope lens), thus reducing the sensitivity of the system to vibrations and/or thermal changes in comparison to regular interferometers. Because of the off-axis holographic recording principle, quantitative phase images of live biosamples can be recorded in a single camera snapshot at full-field geometry without any moving parts. And, the use of water-immersion imaging lenses maximizes the achievable resolution limit. This dual-mode microscope platform is first calibrated using microbeads, then applied to the characterization of fixed cells (neuroblastoma, breast cancer, and hippocampal neuronal cells) and, finally, validated for visualization of dynamic living cells (hippocampal neurons).
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-01-01 |