0000000000010904

AUTHOR

Daniel Krüger

showing 2 related works from this author

Scanning force microscopy based rapid force curve acquisition on supported lipid bilayers: experiments and simulations using pulsed force mode.

2004

In situ pulsed force mode scanning force microscopy (PFM-SFM) images of phase separated solid-supported lipid bilayers are discussed with the help of computer simulations. Simultaneous imaging of material properties and topography in a liquid environment by means of PFM-SFM is severely hampered by hydrodynamic damping of the cantilever. Stiffness and adhesion images of solid-supported membranes consisting of cholesterol, sphingomyelin, and 1,2-dioleyl-phosphatidylcholine obtained in aqueous solution exhibit contrast inversion of adhesion and stiff. ness images depending on parameters such as driving frequency, amplitude, and trigger setting. Simulations using a simple harmonic oscillator mo…

Cantileverbusiness.industryChemistryLipid BilayersPhase (waves)StiffnessSimple harmonic motionMicroscopy Atomic ForceAtomic and Molecular Physics and OpticsSphingomyelinsScanning probe microscopyOpticsCholesterolmedicinePhosphatidylcholinesComputer SimulationPhysical and Theoretical Chemistrymedicine.symptombusinessMaterial propertiesLipid bilayerNon-contact atomic force microscopyChemphyschem : a European journal of chemical physics and physical chemistry
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Scrutiny of the Failure of Lipid Membranes As A Function of Headgroups, Chain Length, and Lamellarity Measured by Scanning Force Microscopy

2004

AbstractA fast, quantitative, and unambiguous screening of material properties of biomembranes using scanning force microscopy in pulsed force mode on lipid membranes is presented. The spatially resolved study of breakthrough force, breakthrough distance, adhesion, stiffness, and topography of lipid membranes as determined simultaneously by digitalized pulsed force mode provides new insight into the structure-function relationship of model membranes, which are systematically analyzed by varying chain length, lipid headgroup, and lamellarity. For this purpose, a novel unbiased analysis method is presented. A strong correlation between adhesion and breakthrough events is found on lipid bilaye…

Macromolecular SubstancesMembrane FluidityLipid BilayersBiophysicsAnalytical chemistryMolecular ConformationMicroscopy Atomic ForceMicromanipulationMotionStructure-Activity RelationshipMicroscopyMaterials TestingmedicineMembrane fluidityLipid bilayerLiposomeMembranesChemistrytechnology industry and agricultureStiffnessMembranes ArtificialElasticityMembraneStructural stabilityLiposomesBiophysicslipids (amino acids peptides and proteins)Stress Mechanicalmedicine.symptomMaterial properties
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