6533b824fe1ef96bd12800e0
RESEARCH PRODUCT
Scanning force microscopy based rapid force curve acquisition on supported lipid bilayers: experiments and simulations using pulsed force mode.
Daniel KrügerAndreas JanshoffStephanie Krügersubject
Cantileverbusiness.industryChemistryLipid BilayersPhase (waves)StiffnessSimple harmonic motionMicroscopy Atomic ForceAtomic and Molecular Physics and OpticsSphingomyelinsScanning probe microscopyOpticsCholesterolmedicinePhosphatidylcholinesComputer SimulationPhysical and Theoretical Chemistrymedicine.symptombusinessMaterial propertiesLipid bilayerNon-contact atomic force microscopydescription
In situ pulsed force mode scanning force microscopy (PFM-SFM) images of phase separated solid-supported lipid bilayers are discussed with the help of computer simulations. Simultaneous imaging of material properties and topography in a liquid environment by means of PFM-SFM is severely hampered by hydrodynamic damping of the cantilever. Stiffness and adhesion images of solid-supported membranes consisting of cholesterol, sphingomyelin, and 1,2-dioleyl-phosphatidylcholine obtained in aqueous solution exhibit contrast inversion of adhesion and stiff. ness images depending on parameters such as driving frequency, amplitude, and trigger setting. Simulations using a simple harmonic oscillator model explain experimental findings and give a deeper insight into the way PFM-SFM experiments have to be performed in order to obtain interpretable results and hence pave the way for reliable material contrast imaging at high speed.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2004-07-19 | Chemphyschem : a European journal of chemical physics and physical chemistry |