Polycyclic aromatic hydrocarbons (PAHs) and their oxygen-containing derivatives (OPAHs) in soils from the Angren industrial area, Uzbekistan.

We measured the concentrations and depth distribution (0-10, 10-20 cm) of 31 PAHs and 12 OPAHs in soils at eleven equidistant sampling points along a 20-km transect in the Angren industrial region (coal mine, power plant, rubber factory, gold mine), Uzbekistan to gain an insight into their concentrations, sources, and fate. Concentrations of all compounds were mostly much higher in the 0-10 cm than in the 10-20 cm layer except in disturbed soil close to the coal mine. Proximity to one of the industrial emitters was the main determinant of PAH and OPAH concentrations. The Sigma31PAHs concentrations correlated positively with the Sigma7 carbonyl-OPAH (r=0.98, p<0.01), Sigma5 hydroxyl-OPAH (r=…

Polycyclic aromatic hydrocarbons and trace metal contamination of coastal sediment and biota from Togo

The state of contamination of tropical environments, particularly in Africa, remains a relatively under explored subject. Here, we determined polycyclic aromatic hydrocarbon (PAH) and trace metal concentrations in coastal sediment and biota samples (fish and mussels) from Togo (West Africa). In the sediments, the ∑21 PAH concentrations ranged from4 ng g(-1) to 257 ng g(-1), averaging 92 ng g(-1). Concentration ratios of low molecular weight PAHs (2-3 rings) versus high molecular weight PAHs (≥4 rings) were always lower than 1 (ranging from 0.08 to 0.46) indicating that high molecular weight PAHs were dominant in all sediment samples, and that PAHs originated mainly from anthropogenic combus…

Isotope ratios of nonexchangeable hydrogen in soils from different climate zones

Abstract The fractionation of H isotopes in global precipitation leads to a continuous decrease in 2 H concentrations of precipitation with increasing latitude, altitude and continentality. Thus, the local precipitation, soil and ground water used by plants for photosynthesis or consumed during neoformation of pedogenic clay minerals often have a spatially diagnostic δ 2 H value. We hypothesize that the δ 2 H value of isotopically nonexchangeable H in bulk soil (composed of C-bonded H in soil organic matter (SOM) and the nonexchangeable fraction of O-bonded H in pedogenic clay minerals) is correlated with the mean δ 2 H value of local precipitation water at the place and time of biomass pro…

A 2600-year record of past polycyclic aromatic hydrocarbons (PAHs) deposition at Holzmaar (Eifel, Germany)

Polycyclic aromatic hydrocarbons (PAHs) are a proxy for climate- and human-related historical fire activity which has rarely been used beyond 1800 AD. We explored the concentration and composition patterns of PAHs together with other proxies (charcoal, C, N, S, δ13C, δ15N, and δ34S) in a sediment core of Holzmaar as indicators of variations in climate and anthropogenic activity over the past 2600 years. The concentrations of pyrogenic PAHs remained low (< 500 ng g− 1) from the pre-Roman Iron Age (600 BC) until the first significant increases to ca. 1000–1800 ng g− 1 between 1700 and 1750 AD related to regional iron production. The highest increases in pyrogenic PAH concentrations occurred w…

Polychlorinated biphenyls (PCBs) in soils of the Moscow region: Concentrations and small-scale distribution along an urban–rural transect

In soils of the Moscow region, we examined PCB concentrations in bulk samples and aggregate fractions. Topsoils under grassland and forest at five locations along a southeast-bound transect from Moscow and at a northeastern background location (grassland only) were analysed. We collected aggregates1 cm and fractionated them into interior and exterior portions and also analysed the remaining soil without the aggregates1 cm. The concentrations of the sum of 17 PCBs (sigma17PCBs) in 35 bulk soil samples ranged from 3.1 to 42 microg kg(-1). This was 48-61% of the sigma33PCBs determined in 23 selected samples. The congeners 138(+158), 101 and 52 were most abundant. All PCB concentrations and the…

Selenium Partitioning and Stable Isotope Ratios in Urban Topsoils

oxides. Th e δ82/76 Se values of total Se in the topsoils were close to the bulk Earth composition with an average δ82/76 Se value of −0.03 ± SD 0.38‰ suggesting that there was no or little Se isotope fractionation in soil. We attribute the small isotope fractionation to the low bioavailability of Se as a consequence of the presence of Fe oxides (adsorbing the dominating Se(IV) forms strongly), organic matter, and SO 4 2− (prevents biouptake of the Se(IV) forms) in the study soils. Small Se isotope fractionations of −0.59 to −0.35‰ in mainly forest soils and of 0.26 to 0.45‰ in mainly alluvial soils were presumably caused by soil/plant-recycling and Se contamination by river water, respecti…

PM2.5-bound oxygenated PAHs, nitro-PAHs and parent-PAHs from the atmosphere of a Chinese megacity: Seasonal variation, sources and cancer risk assessment

Polycyclic aromatic compounds (PACs) in air particulate matter contribute considerably to the health risk of air pollution. The objectives of this study were to assess the occurrence and variation in concentrations and sources of PM2.5 bound PACs [Oxygenated PAHs (OPAHs) nitro PAHs and parent PAHs] sampled from the atmosphere of a typical Chinese megacity (Xi'an) to study the influence of meteorological conditions on PACs and to estimate the lifetime excess cancer risk to the residents of Xi'an (from inhalation of PM2.5 bound PACs). To achieve these objectives we sampled 24 h PM2.5 aerosols (once in every 6days from 5 July 2008 to 8 August 2009) from the atmosphere of Xi'an and measured the…

Net ammonification as influenced by plant diversity in experimental grasslands

Abstract Previous plant diversity experiments have mainly reported positive correlations between diversity and N mineralization. We tested whether this relationship can be explained by plant diversity-induced changes i) in the quantity or quality of organic matter or ii) in microclimatic conditions of central European grassland mixtures. We measured ex-situ net ammonification in a laboratory incubation of aboveground plant material and soil sampled in differently diverse plant mixtures. Secondly, in-situ net ammonification was assessed in a field incubation with mineralization cores containing standardized material in four treatments: soil only (control), and soil mixed with field-fresh pla…

Will nutrient cycles in a tropical montane forest in Ecuador be affected by a changing element composition of rainfall?

Concentrations and forms of heavy metals in Slovak soils

The risk assessment of heavy-metal contamination in soils requires knowledge of the controls of metal concentrations and speciation. We tested the relationship between soil properties (pH, CEC, Corg, oxide concentrations, texture) and land use (forest, grassland, arable) and the partitioning of Al, Cd, Cr, Cu, Fe, Mn, Ni, Pb, and Zn among the seven fractions of a sequential extraction procedure in 146 A horizons from Slovakia. Using a cluster analysis, we identified 92 soils as representing background metal concentrations while the remaining 54 soils showed anthropogenic contamination. Among the background soils, forest soils had the lowest heavy-metal concentrations except for Pb (highest)…

Natural Forest Management in Neotropical Mountain Rain Forests — An Ecological Experiment

In tropical forests, the first step in the destruction cycle is usually the over-exploitation of high value timber, leading not only to extinction of the extracted species (Silva Matos and Bovi 2002) but also to the conversion of the forests into pastures in many cases (Wunder 1996b). In many highlands of Ecuador the productivity of the pastures is depleted due to the invasion of bracken fern (see Chapter 28 in this volume), which finally leads again to the conversion of primary forests by local farmers (Paulsch et al. 2001; Hartig and Beck 2003). This process usually is accompanied by loss of biodiversity (Brooks et al. 200 I), increased erosion, changes of hydrology (Bruijnzel 2004), and …

Response of water and nutrient fluxes to improvement fellings in a tropical montane forest in Ecuador

Abstract Management of natural forests might be one option to reduce the high deforestation rate in Ecuador. We therefore evaluated the response of water and nutrient cycles in a natural tropical montane forest to improvement fellings with the aim of favoring economically valuable target trees which will later be harvested with additional ecosystem impacts not considered here. The study was conducted at ca. 1900–2200 m above sea level in the south Ecuadorian Andes on the east-exposed slope of the east cordillera. In June 2004, one of two paired ca. 10-ha large catchments was thinned by felling 10.2% of the initial basal area (dbh ≥ 10 cm) on 30% of the catchment. The stems remained in situ.…

Plant diversity enhances the natural attenuation of polycyclic aromatic compounds (PAHs and oxygenated PAHs) in grassland soils

Increasing plant species richness stimulates microbial activity in soil, which might favor biodegradation of polycyclic aromatic compounds (PACs). To explore the relationship between plant community composition and PACs in grassland soils (Fluvisols exposed to an urban atmosphere), we determined the concentrations of 29 polycyclic aromatic hydrocarbons (PAHs) and 15 oxygenated PAHs (OPAHs) in topsoils of 80 plots of a grassland biodiversity experiment. The plots included different levels of plant species richness (1, 2, 4, 8, 16, 60 species) and 1–4 plant functional groups (grasses, small herbs, tall herbs, and legumes) in a randomized block design. The concentrations (ng g−1) of ∑29PAHs an…

Plant diversity effects on the water balance of an experimental grassland

In the literature, contrasting effects of plant species richness on the soil water balance are reported. Our objective was to assess the effects of plant species and functional richness and functional identity on soil water contents and water fluxes in the experimental grassland of the Jena Experiment. The Jena Experiment comprises 86 plots on which plant species richness (0, 1, 2, 4, 8, 16, and 60) and functional group composition (zero to four functional groups: legumes, grasses, tall herbs, and small herbs) were manipulated in a factorial design. We recorded meteorological data and soil water contents of the 0·0–0·3 and 0·3–0·7 m soil layers and calculated actual evapotranspiration (ETa)…

Water budgets of three small catchments under montane forest in Ecuador: experimental and modelling approach

The water budget of forested catchments controls the local water supply and influences the regional climate. To assess the anthropogenic impact on the water cycle, we constructed a water budget for three ∼10 ha catchments under lower montane forest on the east-facing slope of the Andes in south Ecuador at 1900–2150 m elevation. We used field hydrological measurements and modelled surface flows with TOPMODEL, a semi-distributed catchment model. We measured incident precipitation, throughfall, stemflow, and surface flow between May 1998 and April 2002 in hourly to weekly resolution, and determined all variables needed to parameterise TOPMODEL. On average, of the four monitored years and three…

Oxygen-containing polycyclic aromatic hydrocarbons (OPAHs) in urban soils of Bratislava, Slovakia: patterns, relation to PAHs and vertical distribution.

Abstract We determined concentrations, sources, and vertical distribution of OPAHs and PAHs in soils of Bratislava. The ∑14 OPAHs concentrations in surface soil horizons ranged 88–2692 ng g−1 and those of ∑34 PAHs 842–244,870 ng g−1. The concentrations of the ∑9 carbonyl-OPAHs (r = 0.92, p = 0.0001) and the ∑5 hydroxyl-OPAHs (r = 0.73, p = 0.01) correlated significantly with ∑34 PAHs concentrations indicating the close association of OPAHs with parent-PAHs. OPAHs were quantitatively dominated by 9-fluorenone, 9,10-anthraquinone, 1-indanone and benzo[a]anthracene-7,12-dione. At several sites, individual carbonyl-OPAHs had higher concentrations than parent PAHs. The concentration ratios of se…

Plant Growth Along the Altitudinal Gradient — Role of Plant Nutritional Status, Fine Root Activity, and Soil Properties

In tropical montane forests, aboveground net primary productivity (ANPP ) usually decreases with increasing altitude. Besides low photosynthesis (Kitayama and Aiba 2002) and direct impact of low temperatures on plant growth (Hoch and Korner 2003), low ANPP at high altitudes has often been attributed to nutrient limitation (Bruijnzeel et al. 1993; Bruijnzeel and Veneklaas 1998; Tanner et al. 1998). Plant growth is often correlated with nutrient availability in tropical montane forests. For example, the exceptionally high tree stature in a montane forest stand in Papua New Guinea was attributed to its nutrient rich soil parent material (Edwards and Grubb 1977). In montane forests of Jamaica (…

Short-term response of the Ca cycle of a montane forest in Ecuador to low experimental CaCl2 additions

The tropical montane forests of the E Andean cordillera in Ecuador receive episodic Sahara-dust inputs particularly increasing Ca deposition. We added CaCl2 to isolate the effect of Ca deposition by Sahara dust to tropical montane forest from the simultaneously occurring pH effect. We examined components of the Ca cycle at four control plots and four plots with added Ca (2 x 5kg ha(-1) Ca annually as CaCl2) in a random arrangement. Between August 2007 and December 2009 (four applications of Ca), we determined Ca concentrations and fluxes in litter leachate, mineral soil solution (0.15 and 0.30 m depths), throughfall, and fine litterfall and Al concentrations and speciation in soil solutions…

Resources, recruitment limitation and invader species identity determine pattern of spontaneous invasion in experimental grasslands

Summary 1 A number of experimental studies have supported the hypothesis that diversity increases invasion resistance, but several mechanisms were proposed to explain this relationship. We studied spontaneous invasion in experimental grasslands varying in species richness (1–16 (60)) and plant functional group richness and identity (1–4; grasses, legumes, tall herbs small herbs) during the first 3 years after establishment on large-area plots of 20 × 20 m size. 2 Invader species number, biomass and density decreased with increasing community species richness. The identity of successful invaders changed through time as the relative importance of external invaders (= species not belonging to …

Global patterns of polycyclic aromatic hydrocarbons (PAHs) in soil

Abstract Recent findings suggest a higher natural contribution to PAH concentrations in soil than previously thought. I hypothesized that the PAH pattern in soil is dominated by two main types, which are indicative of background conditions on the one side (i.e., by biological and diffuse PAHs) and a strong impact by atmospheric deposition of anthropogenic emissions on the other side. To test this hypothesis, concentrations of 20 PAHs in 225 topsoil samples from 12 geographic regions were evaluated. The Σ20PAHs concentrations ranged between 4.8 and 186,000 μg kg− 1. In soils with low concentrations of Σ20PAHs, naphthalene (NAPH), phenanthrene (PHEN), and perylene (PERY), together defined as …

Tropical Andean forest derives calcium and magnesium from Saharan dust

[1] We quantified base metal deposition to Amazonian montane rain forest in Ecuador between May 1998 and April 2003 and assessed the response of the base metal budget of three forested microcatchments (8–13 ha). There was a strong interannual variation in deposition of Ca [4.4–29 kg ha−1 a−1], Mg [1.6–12], and K [9.8–30]). High deposition changed the Ca and Mg budgets of the catchments from loss to retention, suggesting that the additionally available Ca and Mg was used by the ecosystem. Increased base metal deposition was related to dust outbursts of the Sahara and an Amazonian precipitation pattern with trans-regional dry spells allowing for dust transport to the Andes. The increased base…

Tropical Andean forests are highly susceptible to nutrient inputs--rapid effects of experimental N and P addition to an Ecuadorian montane forest.

Tropical regions are facing increasing atmospheric inputs of nutrients, which will have unknown consequences for the structure and functioning of these systems. Here, we show that Neotropical montane rainforests respond rapidly to moderate additions of N (50 kg ha -1 yr -1) and P (10 kg ha -1 yr -1). Monitoring of nutrient fluxes demonstrated that the majority of added nutrients remained in the system, in either soil or vegetation. N and P additions led to not only an increase in foliar N and P concentrations, but also altered soil microbial biomass, standing fine root biomass, stem growth, and litterfall. The different effects suggest that trees are primarily limited by P, whereas some pro…

Periglacial transport distance of Pb derived from small-scale ore veins in the Rhenish Slate Mountains

Abstract The spatial extent of metal contamination caused by small-scale ore veins is increased by periglacial solifluction. Our objectives were (1) to examine the spatial distribution of Pb in cover beds that migrated over Pb ore veins, (2) to calculate the transport distance of ore-derived Pb, and (3) to determine controls on the transport distance. We examined six transects (320–775 m long) in the Rhenish Slate Mountains in West Germany. The transects included four west-exposed and two east-exposed slopes with inclinations between 2–20°. All transects were forest-covered and located at 280–450 m above sea level. Soils were Dystric Cambisols and Stagnic Luvisols. Samples of 120 B horizons…

Naphthalene production by microorganisms associated with termites: Evidence from a microcosm experiment

Abstract There have been several reports published which suggest that it is possible that the polycyclic aromatic hydrocarbons (PAHs) naphthalene (NAPH), phenanthrene (PHEN) and perylene (PERY) in tropical environments have a biological source. This source might be related to the activity of termites or their associated microorganisms. We aimed to provide direct evidence for the biological production of NAPH, PHEN and PERY by conducting microcosm experiments in the State of Tocantins, Brazil, in which termite nests (with or without termites) were placed in an enclosed environment in which we controlled all PAH fluxes and monitored changes of PAH stocks. The experiments were carried out with…

Spatial throughfall heterogeneity in a montane rain forest in Ecuador: Extent, temporal stability and drivers

Summary The drivers of spatial throughfall heterogeneity are still not fully understood. At an undisturbed forest site in the Ecuadorian Andes with ca. 2600 mm of annual rainfall we determined the accuracy of throughfall measurements by comparing Hellmann-type funnel gauges with troughs. At the same undisturbed and a managed, selectively-logged forest site we determined spatial variability of throughfall, temporal stability of spatial variability and the controls of spatial throughfall variability using a 4-year dataset in weekly resolution. There were no systematic differences between the collected volumes of funnel gauges and troughs. Based on the statistical distribution of annual throug…

Copper Isotope Fractionation during Complexation with Insolubilized Humic Acid

The bioavailability, mobility, and toxicity of Cu depend on Cu speciation in solution. In natural systems like soils, sediments, lakes, and river waters, organo-Cu complexes are the dominating species. Organo-complexation of Cu may cause a fractionation of stable Cu isotopes. The knowledge of Cu isotope fractionation during sorption on humic acid may help to better understand Cu isotope fractionation in natural environments and thus facilitate the use of Cu stable isotope ratios (delta(65)Cu) as tracer of the fate of Cu in the environment. We therefore studied Cu isotope fractionation during complexation with insolubilized humic acid (IHA) as a surrogate of humic acid in soil organic matter…

Isotopes Trace Biogeochemistry and Sources of Cu and Zn in an intertidal soil

River floodplain soils are sinks and potential sources for toxic trace metals like Cu and Zn. We hypothesize that stable Cu and Zn isotope ratios reflect both the mobilization and the sources of metals. We determined the soil properties, the concentrations and partitioning of Cu and Zn, and variations in δ65Cu and δ66Zn values in a core obtained from an Aquic Udifluvent developed on a freshwater intertidal mudflat of the River Elbe, Germany. The core was sampled at 2 cm intervals to a depth of 34 cm, which corresponds to approximately 9 yr of sedimentation. Elevated concentrations of Cu (up to 320 μg g−1) and Zn (up to 2080 μg g−1) indicated anthropogenic pollution. At the time of sampling …

Plant diversity effects on aboveground and belowground N pools in temperate grassland ecosystems: Development in the first 5 years after establishment

[1] Biodiversity is expected to improve ecosystem services, e.g., productivity or seepage water quality. The current view of plant diversity effects on element cycling is based on short-term grassland studies that discount possibly slow belowground feedbacks to aboveground diversity. Furthermore, these grasslands were established on formerly arable land associated with changes in soil properties, e.g., accumulation of organic matter. We hypothesize that the plant diversity-N cycle relationship changes with time since establishment. We assessed the relationship between plant diversity and (1) aboveground and soil N storage and (2) NO3-N and NH4-N availability in soil between 2003 and 2007 in…

Stable Cu and Zn isotope ratios as tracers of sources and transport of Cu and Zn in contaminated soil

Abstract Copper and Zn metals are produced in large quantities for different applications. During Cu production, large amounts of Cu and Zn can be released to the environment. Therefore, the surroundings of Cu smelters are frequently metal-polluted. We determined Cu and Zn concentrations and Cu and Zn stable isotope ratios (δ 65 Cu, δ 66 Zn) in three soils at distances of 1.1, 3.8, and 5.3 km from a Slovak Cu smelter and in smelter wastes (slag, sludge, ash) to trace sources and transport of Cu and Zn in soils. Stable isotope ratios were measured by multicollector inductively coupled plasma mass spectrometry (MC-ICP-MS) in total digests. Soils were heavily contaminated with concentrations u…

Nitrogen and phosphorus budgets in experimental grasslands of variable diversity.

Previous research has shown that plant diversity influences N and P cycles. However, the effect of plant diversity on complete ecosystem N and P budgets has not yet been assessed. For 20 plots of artificially established grassland mixtures differing in plant diversity, we determined N and P inputs by bulk and dry deposition and N and P losses by mowing (and subsequent removal of the biomass) and leaching from April 2003 to March 2004. Total deposition of N and P was 2.3 +/- 0.1 and 0.2 +/- 0.01 g m(-2) yr(-1), respectively. Mowing was the main N and P loss. The net N and P budgets were negative (-6.3 +/- 1.1 g N and -1.9 +/- 0.2 g P m(-2) yr(-1)). For N, this included a conservative estimat…

Method optimization to measure polybrominated diphenyl ether (PBDE) concentrations in soils of Bratislava, Slovakia.

We modified an analytical method to determine polybrominated diphenyl ethers (PBDEs) in urban soils of Bratislava (Slovakia). Gel permeation chromatography (GPC) introduced as a clean-up step for soil extracts substantially reduced matrix enhancements when PBDEs were measured with gas chromatography-negative chemical ionization-mass spectrometry (GC-NCI-MS). The resulting method proved to be accurate, precise, and showed low detection limits. The sum of 15 PBDE concentrations in surface horizons of Bratislava soils ranged from 87 to 627 pg g(-1). PBDE concentrations were mostly higher in surface than deeper horizons probably because of atmospheric deposition and lack of substantial vertical…

Response of the N and P cycles of an old-growth montane forest in Ecuador to experimental low-level N and P amendments

Abstract Atmospheric nitrogen (N) and phosphorus (P) depositions are expected to increase in the tropics as a consequence of increasing human activities in the next decades. In the literature, it is frequently assumed that tropical montane forests are N-limited, while tropical lowland forests are P-limited. In a low-level N and P addition experiment, we determined the short-term response of N and P cycles in a north Andean montane forest on Palaeozoic shists and metasandstones at an elevation of 2100 m a.s.l. to increased N and P inputs. We evaluated experimental N, P and N + P additions (50 kg ha −1  yr −1 of N, 10 kg ha −1  yr −1 of P and 50 kg + 10 kg ha −1  yr −1 of N and P, respectivel…

Soil properties and tree growth along an altitudinal transect in Ecuadorian tropical montane forest

In tropical montane forests, soil properties change with increasing altitude, and tree-growth decreases. In a tropical montane forest in Ecuador, we determined soil and tree properties along an altitudinal transect between 1960 and 2450 m asl. In different vegetation units, all horizons of three replicate profiles at each of eight sites were sampled and height, basal area, and diameter growth of trees were recorded. We determined pH and total concentrations of Al, C, Ca, K, Mg, Mn, N, Na, P, S, Zn, polyphenols, and lignin in all soil horizons and in the mineral soil additionally the effective cation-exchange capacity (CEC). The soils were Cambisols, Planosols, and Histosols. The concentrati…

A Method to Quantitatively Trap Volatilized Organoselenides for Stable Selenium Isotope Analysis

If volatile organoselenides are to be analyzed for their stable Se isotope composition to elucidate sources and formation processes, organoselenides need to be trapped quantitatively to avoid artificial Se isotope fractionation. We developed an efficient trap of organoselenides to be used in microcosms designed to determine the Se isotope fractionation by microbial transformation of inorganic Se to volatile organoselenides. The recoveries of volatilized dimethyldiselenide (DMDSe) from aqueous standard solutions by activated charcoal and alkaline peroxide solution with subsequent freeze-drying and purification via a cation exchange resin were tested. Microcosm experiments with the Se-methyla…

Soils Along the Altitudinal Transect and in Catchments

Nutrient Status and Fluxes at the Field and Catchment Scale

Amazonian biomass burning-derived acid and nutrient deposition in the north Andean montane forest of Ecuador

[1] We explored the influence of biomass burning in Amazonia and northeastern Latin America on N, C, P, S, K, Ca, Mg, Al, Mn, and Zn cycles of an Andean montane forest in south Ecuador exposed to the Amazon basin between May 1998 and April 2003. We assessed the response of the element budget of three microcatchments (8–13 ha) to the variations in atmospheric deposition between the intensive burning season and outside the burning season in Amazonia. There were significantly elevated H, N, and Mn depositions during biomass burning. Elevated H deposition during biomass burning caused elevated base metal loss from the canopy and the organic horizon and deteriorated already low base metal supply…

Reply to the comment of Zimmermann et al. (2010) on “Spatial throughfall heterogeneity in a montane rain forest in Ecuador: Extent, temporal stability and drivers” [J. Hydrol. 377 (2009), 71–79]

Stable Copper Isotopes: A Novel Tool to Trace Copper Behavior in Hydromorphic Soils

Copper is an essential micronutrient for all organisms but may also be a pollutant. We studied the natural abundance of stable Cu isotope ratios in four soils to test whether δ 65 Cu values can be used as a tracer for biogeochemical processes in hydromorphic soils. Two of the soils were affected by stagnant water and the other two by groundwater. We determined standard soil properties and Cu partitioning into seven fractions of a sequential extraction. Copper stable isotope ratios were measured in total soil digests with multicollector inductively coupled plasma mass spectrometry. Copper concentrations in the study soils were low to average (5-34 mg kg -1 ). The variation in Cu isotope rati…

Water flow paths in soil control element exports in an Andean tropical montane forest

We tested the hypothesis that concentrations of chemical constituents in stream water can be explained by the depth of water flow through soil. Therefore, we measured the concentrations of total organic carbon (TOC), NO 3 -N, NH 4 -N, dissolved organic nitrogen (DON), P, S, K, Ca, Mg, Na, Al and Mn in rainfall, throughfall, stemflow, litter leachate, mineral soil solution and stream water of three 8-13 ha catchments on steep slopes (1900-2200 m above sea level) of the south Ecuadorian Andes, from April 1998 to April 2003. Peak C (14-22 mg litre -1 ), N (0.6-0.9 mg litre -1 ), K (0.5-0.7 mg litre -1 ), Ca (0.6-1.0 mg litre -1 ), Mg (0.3-0.5 mg litre -1 ), Al (110-390 μg litre -1 ) and Mn (3.…

Nitrate leaching in soil: Tracing the NO3− sources with the help of stable N and O isotopes

Abstract Legumes increase the plant-available N pool in soil, but might also increase NO3− leaching to groundwater. To minimize NO3− leaching, N-release processes and the contribution of legumes to NO3− concentrations in soil must be known. Our objectives were (1) to quantify NO3−-N export to >0.3 m soil depth from three legume monocultures (Medicago x varia Martyn, Onobrychis viciifolia Scop., Lathyrus pratensis L.) and from three bare ground plots. Furthermore, we (2) tested if it is possible to apply a mixing model for NO3− in soil solution based on its dual isotope signals, and (3) estimated the contribution of legume mineralization to NO3− concentrations in soil solution under field co…

Does plant diversity influence phosphorus cycling in experimental grasslands?

Plant diversity was shown to influence the N cycle, but plant diversity effects on other nutrients remain unclear. We tested whether plant species richness or the presence/absence of particular functional plant groups influences P partitioning among differently extractable pools in soil, P concentrations in soil solution, and exploitation of P resources (i.e. the proportion of total bioavailable P in plants and soil that was stored in aboveground biomass) by the plant community in a 5-year biodiversity experiment in grassland.The experimental grassland site established in 2002 had 82 plots with different combinations of numbers of species (1, 2, 4, 8, 16, 60) and functional groups (grasses,…

Towards a new generation of high-resolution meteorological input data for small-scale hydrologic modeling

Summary Current and future challenges of hydrologic sciences are to accurately predict and assess climate-driven impacts on water resources for the relevant scales of planning. However, process-based small-scale hydrologic modeling is data demanding and large uncertainties exist in data-sparse areas. The aim of our study was to test the applicability of the COSMO-DE analysis data (COSMO-DE-A) for hydrologic modeling. COSMO-DE-A data are a new meteorological data set with high temporal and spatial resolution that originates from the German Weather Service data assimilation system using the COSMO-DE weather prediction model. We collected field parameters in a small (10 km 2 ) mountainous catc…

Spatial and temporal patterns of throughfall quantity and quality in a tropical montane forest in Ecuador

Summary In forests, complex canopy processes control the change in volume and chemical composition of rain water. We hypothesize that (i) spatial patterns, (ii) the temporal stability of spatial patterns, and (iii) the temporal course of solute concentrations can be used to explore these processes. The study area at 1950 m above sea level in the south Ecuadorian Andes is far away from anthropogenic emission sources and marine influences. It received ca. 2200 mm of rain annually. We collected rain and throughfall on an event and within-event basis for five precipitation periods between August and October 2005 at up to 25 sites and analyzed the samples for pH and concentrations of K, Na, Ca, …

Nutrient Additions Affecting Matter Turnover in Forest and Pasture Ecosystems

Nutrient inputs into ecosystems of the tropical mountain rainforest region are projected to further increase in the next decades. To investigate whether important ecosystem services such as nutrient cycling and matter turnover in native forests and pasture ecosystems show different patterns of response, two nutrient addition experiments have been established: NUMEX in the forest and FERPAST at the pasture. Both ecosystems already responded 1.5 years after the start of nutrient application (N, P, NP, Ca). Interestingly, most nutrients remained in the respective systems. While the pasture grass was co-limited by N and P, most tree species responded to P addition. Soil microbial biomass in the…

Soil and Plant Nitrogen Pools as Related to Plant Diversity in an Experimental Grassland

Increasing plant species richness decreases soil NO 3 - concentrations in experimental plant mixtures, but the role of particular plant functional groups has remained unclear. Most analyses have focused on particular times of the year or were restricted to NO 3 - . We tested whether plant species richness or particular plant functional groups affect the size of plant-available N pools in soil (KCl-extractable NO 3 - , dissolved inorganic N and organic N [DON] and total dissolved N [TDN] in soil solution) and N concentrations and pools in aboveground biomass. Furthermore, we assessed seasonal variations in the effects of plant species richness and plant functional groups. The experimental gr…

Isotope Fractionation of Selenium During Fungal Biomethylation by Alternaria alternata

The natural abundance of stable Se isotopes may reflect sources and formation conditions of methylated Se. We aimed at (1) quantifying the degree of methylation of selenate [Se(VI)] and (hydro)selenite [Se(IV)] by the fungus Alternaria alternata at pH 4 and 7 and (2) determining the effects of these different Se sources and pH values on 82Se/76Se ratios (δ82/76Se) in methylselenides. Alternaria alternata was incubated with Se(VI) and Se(IV) in closed microcosms for 11-15 days and additionally with Se(IV) for 3-5 days at 30 °C. We determined Se concentrations and δ82/76Se values in source Se(VI) and Se(IV), media, fungi, and trapped methylselenides. In Se(VI) incubations, methylselenide vola…

Stable Cu isotope fractionation in soils during oxic weathering and podzolization

Abstract Copper stable isotope ratios are fractionated during various biogeochemical processes and may trace the fate of Cu during long-term pedogenetic processes. We assessed the effects of oxic weathering (formation of Cambisols) and podzolization on Cu isotope ratios (δ65Cu). Two Cambisols (oxic weathered soils without strong vertical translocations of soil constituents) and two Podzols (soils showing vertical translocation of organic matter, Fe and Al) were analyzed for Cu concentrations, partitioning of Cu in seven fractions of a sequential extraction and δ65Cu values in bulk soil. Cu concentrations in the studied soils were low (1.4–27.6 μg g−1) and Cu was mainly associated with stron…

Comparison of Different Techniques for the Measurement of Precipitation in Tropical Montane Rain Forest Regions

Abstract Characteristics of different precipitation measurements in a tropical mountain valley in southern Ecuador are compared in this study to determine potential errors. The instruments are used for different ecological purposes like erosion studies, through fall measurements, investigation of atmospheric chemistry, and modeling of area rainfall distribution. Five recording devices (two precipitation radars, an electro-optical present weather sensor, and two tipping buckets) and three totaling gauges were operated in parallel at a designated site. Data were taken between 1998 and 2003 with different temporal resolution and different operational periods. The general agreement between the …

Analysis of Polycyclic Aromatic Hydrocarbons and Their Oxygen-Containing Derivatives and Metabolites in Soils

Although polycyclic aromatic hydrocarbons (PAHs) have been extensively studied, the knowledge of their oxygen-containing derivatives and metabolites (OPAHs) in soils is limited. We modified and tested an existing analytical protocol involving pressurized liquid extraction of soil followed by fractionation of target compounds into PAHs and OPAHs on a silica gel column and gas chromatography/ mass spectrometry-based separation and quantification. Polycyclic aromatic hydrocarbons and carbonyl-OPAHs were quantified directly after separation on silica gel columns, and hydroxyl/carboxyl-OPAHs were quantified after silylation with N,O-bis(trimethylsilyl)trifluoroacetamide. Recoveries between 78 an…