Early Plasmodium-induced inflammation does not accelerate aging in mice

10 pages; International audience; Aging is associated with a decline of performance leading to reduced reproductive output and survival. While the antagonistic pleiotropy theory of aging has attracted considerable attention, the molecular/physiological functions underlying the early-life benefits/late-life costs paradigm remain elusive. We tested the hypothesis that while early activation of the inflammatory response confers benefits in terms of protection against infection, it also incurs costs in terms of reduced reproductive output at old age and shortened longevity. We infected mice with the malaria parasite Plasmodium yoelii and increased the inflammatory response using an anti-IL-10 r…

Immune activation increases susceptibility to oxidative tissue damage in Zebra Finches.

Summary 1The innate immune response involves the production of highly reactive molecules (reactive oxygen and nitrogen species, ROS and RNS). These toxic compounds can effectively destroy invading pathogens but can also, non-specifically, target host cells. Tissue damage caused by ROS and RNS can be substantial if the inflammatory response is overactive, potentially inducing a so-called immunopathology. 2In this study, we induced an inflammatory response in Zebra Finches by the way of a LPS injection (lipopolysaccharide of the cell wall of Escherichia coli), using as a control a group of birds injected with saline (phosphate-buffered saline). Body mass was measured both before and 24 h afte…

Social interactions modulate the virulence of avian malaria infection

There is an increasing understanding of the context-dependent nature of parasite virulence. Variation in parasite virulence can occur when infected individuals compete with conspecifics that vary in infection status; virulence may be higher when competing with uninfected competitors. In vertebrates with social hierarchies, we propose that these competition-mediated costs of infection may also vary with social status. Dominant individuals have greater competitive ability than competing subordinates, and consequently may pay a lower prevalence-mediated cost of infection. In this study we investigated whether costs of malarial infection were affected by the occurrence of the parasite in compet…

Modification of the rat aortic wall during ageing; possible relation with decrease of peptidergic innervation.

Structural changes of the male rat aorta were followed from birth to old age in male and female rats. In males, the vessel media width and area progressively increase concomitantly with a decrease of nuclei density during ageing, suggesting an hypertrophy of the smooth muscle cells. These correlations were however not evidenced in females. TUNEL-positive cells were found in media of 4 and 6 months in both sexes, mainly on the luminal side and in the adventitia. When biochemical markers were investigated with immunohistochemistry, media was uniformly stained by the anti-vimentin and anti-alpha-smooth actin at all stages investigated. On the contrary, the surface of media stained with anti-de…

Un point sur la zone de contact entre les deux contrefaisants Hippolais icterina et Hippolais polyglotta.

Le cas du Grand contrefaisant (Hippolais icterina) et du Petit contrefaisant (Hippolais polyglotta) en sympatrie représente un contexte de zone d’hybridation « mobile » particulièrement intéressant d’un point de vue écologique et évolutif. Les travaux de Camille Ferry ont apporté une somme de connaissances importante sur le contexte écologique et comportemental de la vie en sympatrie pour ces deux espèces et initié plusieurs recherches dédiées en particulier aux conséquences morphologiques et vocales de la coexistence sympatrique des deux contrefaisants. Ces recherches montrent notamment des modifications morphologiques chez H. icterina dans le sens d’une plus grande ressemblance avec H. po…

Parental experience of a risky environment leads to improved offspring growth rate.

Abstract Parasites (or diseases) are major selective force for the evolution of life history traits and parasite-host evolution. Mothers can show a variety of responses to parasites during pregnancy with different consequences for them or their offspring. However, whether information in the maternal environment before pregnancy can cause a change in the phenotype of the offspring is unknown. To avoid the confounding effect of pathogens and to reduce the risk of direct effect of mother's immune activation, we injected female laboratory mice with lipopolysaccharides (LPS) before mating. In order to provide a constant information on the potential infectious risk of the environment, females wer…

Effects of harvesting timber stands on goshawk nesting in two European areas

We evaluated the effects of harvesting timber stands on goshawk (Accipiter gentilis) nesting in two European areas (central Italy and eastern France), by studying their occupancy and reproductive performance. We found no difference in the productivity of goshawk pairs reproducing in unlogged vs. logged stands. When considering the same nesting stand, before and after timber harvesting, we noted no differences in the number of young per breeding pair nora year effect. We observed that 87.5% of goshawk pairs nesting in logged stands moved away only when the original stand structure was altered by > 30%, and then only to the nearest neighbouring mature stand (maximum distance ca. 1.5 km). The …

Editorial: Bourgogne Franche-Comté Nature est une revue scientifique

National audience; Face aux divers discours sur la crise environnementale et ses enjeux, l’actualité nous rappelle régulièrement comment le constat scientifique est mis au défi de la désinformation. Celle-ci s’exprime en pervertissant auprès du grand public l'espace offert par le doute critique (par exemple par les soi-disant climatosceptiques, les industriels de la chimie, etc.), ou par l'agora sans filtre d'internet où tous les avis apparaissent se valoir et entretiennent la confusion entre opinion et savoir. Les naturalistes, dans ce contexte, ont un rôle premier à jouer, en produisant des faits vérifiés, et donc en défendant publiquement la démarche et les connaissances scientifiques. L…

Patterns of morphological variation in two sexually dimorphic bird species with different tail shapes

Many studies have focused on tail ornamentation in birds, but not all tail shapes have been studied in depth. Graduated and pin tails have received less attention than forked tails, despite being more likely, in terms of aerodynamic theory, to be honest signals. We report morphological variation in live specimens of two sexually dimorphic passerines from the same site with different tail shapes: graduated (Cape sugarbird Promerops cafer ) and pin (orangebreasted sunbird Antobaphes violacea ). Coefficients of variation (CVs) were calculated for all morphological traits, both non-ornamental (range 1.91–5.72) and ornamental (range 5.83–21.71). Males and females did not differ in CV for any non…

Enrichissement de schéma multidimensionnel en constellation grâce à la Classification Ascendante Hiérarchique

National audience; Les hiérarchies sont des structures cruciales dans un entrepôt de don-nées puisqu'elles permettent l'agrégation de mesures dans le but de proposer une vue analytique plus ou moins globale sur les données entreposées, selon le niveau hiérarchique auquel on se place. Cependant, peu de travaux s'intéressent à la construction de hiérarchies, via un algorithme de fouille de données, pre-nant en compte le contexte multidimensionnel de la dimension concernée. Dans cet article, nous proposons donc un algorithme, implémenté sur une architecture ROLAP, permettant d'enrichir une dimension avec des données factuelles.

Distribution and abundance patterns of a newly colonizing species in Tunisian oases: the Common Blackbird Turdus merula

We investigated distribution and abundance patterns of a recently arrived species in the oases of southern Tunisia: the Common Blackbird Turdus merula. In this system, we expected that the vegetation structure and geographical locations of oases would have played an important part in shaping the patterns of oasis occupancy and local abundance. Using data collected at 53 oases during two breeding seasons, we found that Blackbird occurrence in a given oasis depended mainly on its presence at neighbouring oases. However, vegetation structure did not prove to be a relevant predictor of Blackbird occurrence. Within occupied oases, local abundance was associated with vegetation structure, namely …

Disrupting immune regulation incurs transient costs in male reproductive function.

9 pages; International audience; BACKGROUND: Immune protection against pathogenic organisms has been shown to incur costs. Previous studies investigating the cost of immunity have mostly focused on the metabolic requirements of immune maintenance and activation. In addition to these metabolic costs, the immune system can induce damage to the host if the immune response is mis-targeted or over-expressed. Given its non-specific nature, an over-expressed inflammatory response is often associated with substantial damage for the host. Here, we investigated the cost of an over-expressed inflammatory response in the reproductive function of male mice. METHODOLOGY/PRINCIPAL FINDINGS: We experimenta…

Une nouvelle approche mixte d'enrichissement de dimensions dans un schéma multidimensionnel en constellation Application à la biodiversité des oiseaux

International audience; Les entrepôts de données (DW) et les systèmes OLAP sont des technologies d'analyse en ligne pour de grands volumes de données, basés sur les be-soins des utilisateurs. Leur succès dépend essentiellement de la phase de conception où les exigences fonctionnelles sont confrontées aux sources de données (méthodologie de conception mixte). Cependant, les méthodes de conception existantes semblent parfois inefficaces, lorsque les décideurs définissent des exi-gences fonctionnelles qui ne peuvent être déduites à partir des sources de don-nées (approche centrée sur les données), ou lorsque le décideur n'a pas intégré tous ces besoins durant la phase de conception (approche c…

Supplementary methods and figures from Aging parasites produce offspring with poor fitness prospects

A full description of the methods used to infect mice, to culture parasitic larvae, count parasite eggs and adult worms.

Food availability and competition do not modulate the costs of Plasmodium infection in dominant male canaries.

7 pages; International audience; Understanding the different factors that may influence parasite virulence is of fundamental interest to ecologists and evolutionary biologists. It has recently been demonstrated that parasite virulence may occur partly through manipulation of host competitive ability. Differences in competitive ability associated with the social status (dominant or subordinate) of a host may determine the extent of this competition-mediated parasite virulence. We proposed that differences between subordinate and dominant birds in the physiological costs of infection may change depending on the level of competition in social groups. We observed flocks of domestic canaries to …

New method for field studies on the parapatric distribution of sibling species

Abstract Spatial segregation (parapatry) often occurs between closely related species. The distributions of the two species are sometimes defined with a small overlapping zone (called a `sympatric area') which generally shifts. Exclusion is necessary to explain the persistence and shift of such a spatial pattern. Field studies are carried out to identify the type of interaction that leads to the required exclusion. This is usually achieved by estimating competition and predation parameters to define the type of interaction strong enough to imply exclusion. But interaction parameters are estimated by quantitative methods which require prolonged observation (5–10 years). These estimations are…

Why Does COVID-19 Case Fatality Rate Vary Among Countries?

SummaryBackgroundWhile the epidemic of SARS-CoV-2 is spreading worldwide, there is much concern over the mortality rate that the infection induces. Available data suggest that COVID-19 case fatality rate varies temporally (as the epidemic progresses) and spatially (among countries). Here, we attempted to identify key factors possibly explaining the variability in case fatality rate across countries.MethodsWe used data on the temporal trajectory of case fatality rate provided by the European Center for Disease Prevention and Control, and country-specific data on different metrics describing the incidence of known comorbidity factors associated with an increased risk of COVID-19 mortality at …

The design of artificial nestboxes for the study of secondary hole-nesting birds: A review of methodological inconsistencies and potential biases

The widespread use of artificial nestboxes has led to significant advances in our knowledge of the ecology, behaviour and physiology of cavity nesting birds, especially small passerines. Nestboxes have made it easier to perform routine monitoring and experimental manipulation of eggs or nestlings, and also repeatedly to capture, identify and manipulate the parents. However, when comparing results across study sites the use of nestboxes may also introduce a potentially significant confounding variable in the form of differences in nestbox design amongst studies, such as their physical dimensions, placement height, and the way in which they are constructed and maintained. However, the use of …

Physiological stress does not increase with urbanization in European blackbirds: Evidence from hormonal, immunological and cellular indicators

Urbanization changes the landscape structure and ecological processes of natural habitats. While urban areas expose animal communities to novel challenges, they may also provide more stable environments in which environmental fluctuations are buffered. Species´ ecology and physiology may determine their capacity to cope with the city life. However, the physiological mechanisms underlying organismal responses to urbanization, and whether different physiological systems are equally affected by urban environments remain poorly understood. This severely limits our capacity to predict the impact of anthropogenic habitats on wild populations. In this study, we measured indicators of physiological…

Suppressing an Anti-Inflammatory Cytokine Reveals a Strong Age-Dependent Survival Cost in Mice

7 pages; International audience; BACKGROUND: The central paradigm of ecological immunology postulates that selection acts on immunity as to minimize its cost/benefit ratio. Costs of immunity may arise because the energetic requirements of the immune response divert resources that are no longer available for other vital functions. In addition to these resource-based costs, mis-directed or over-reacting immune responses can be particularly harmful for the host. In spite of the potential importance of immunopathology, most studies dealing with the evolution of the immune response have neglected such non resource-based costs. To keep the immune response under control, hosts have evolved regulat…

Merles noirs Turdus merula urbains et forestiers. Comparaison parasitaire et immunologique.

8 pages; National audience

Reaction norms of host immunity, host fitness and parasite performance in a mouse - intestinal nematode interaction.

8 pages; International audience; The outcome of the encounter between a host and a parasite depends on the synergistic effects of the genetics of the two partners and the environment (sensulato) where the interaction takes place. Reaction norms can depict how host and parasite traits vary across environmental ranges for different genotypes. Here, we performed a large scale experiment where three strains of laboratory mice (SJL, BALB/c and CBA) were infected with four doses of the intestinal nematode Heligmosomoides polygyrus. An increasing infective dose can be considered as a proxy for the environment-dependent risk incontracting the infection. We looked at the fitness traits of hosts and …

The Hierarchical Agglomerative Clustering with Gower index: a methodology for automatic design of OLAP cube in ecological data processing context

In Press, Corrected Proof; International audience; The OLAP systems can be an improvement for ecological studies. In fact, ecology studies, follows and analyzes phenomenon across space and time and according to several parameters. OLAP systems can provide to ecologists browsing in a large dataset. One focus of the current research on OLAP system is the automatic design of OLAP cubes and of data warehouse schemas. This kind of works makes accessible OLAP technology to non information technology experts. But to be efficient, the automatic OLAP building must take into account various cases. Moreover the OLAP technology is based on the concept of hierarchy. Thereby the hierarchical clustering m…

Demographic responses to oxidative stress and inflammation in the wandering albatross (Diomedea exulans).

12 pages; International audience; One of the major challenges in ecological research is the elucidation of physiological mechanisms that underlie the demographic traits of wild animals. We have assessed whether a marker of plasma oxidative stress (TBARS) and plasma haptoglobin (protein of the acute inflammatory phase response) measured at time t predict five demographic parameters (survival rate, return rate to the breeding colony, breeding probability, hatching and fledging success) in sexually mature wandering albatrosses over the next four years (Diomedea exulans) using a five-year individual-based dataset. Non-breeder males, but not females, having higher TBARS at time t had reduced fut…

Accumulation and effects of Cd, Pb and Zn in blackbirds (Turdus merula) along a heavy metal pollution gradient.

Immunity and the emergence of virulent pathogens.

6 pages; International audience; The emergence/re-emergence of infectious diseases has been one of the major concerns for human and wildlife health. In spite of the medical and veterinary progresses as to prevent and cure infectious diseases, during the last decades we have witnessed the emergence/re-emergence of virulent pathogens that pose a threat to humans and wildlife. Many factors that might drive the emergence of these novel pathogens have been identified and several reviews have been published on this topic in the last years. Among the most cited and recognized drivers of pathogen emergence are climate change, habitat destruction, increased contact with reservoirs, etc. These factor…

Variation in clutch size in relation to nest size in birds

© 2014 The Authors. Nests are structures built to support and protect eggs and/or offspring from predators, parasites, and adverse weather conditions. Nests are mainly constructed prior to egg laying, meaning that parent birds must make decisions about nest site choice and nest building behavior before the start of egg-laying. Parent birds should be selected to choose nest sites and to build optimally sized nests, yet our current understanding of clutch size-nest size relationships is limited to small-scale studies performed over short time periods. Here, we quantified the relationship between clutch size and nest size, using an exhaustive database of 116 slope estimates based on 17,472 nes…

Competition for resources modulates cell-mediated immunity and stress hormone level in nestling collared doves (Streptopelia decaocto)

International audience; Competitive stress imposed by hatching asynchrony may affect developmental trajectories of offsprings by regulating resource allocation between growth and other fitness-related traits. For instance, the down-regulation of immunity is a commonly observed phenomenon under stressful conditions. However, physiological mechanisms that regulate resources allocation to growth and immune functions in response to competition for resources, as well as inter-sexual differences in physiological strategies, are still poorly investigated. To partially fill this gap, we first conducted a descriptive study on chicks of the collared dove (Streptopelia decaocto), a species producing t…

Survival cost of an early immune soliciting in nature.

8 pages; International audience; If immune functions confer obvious benefits to hosts, life-history theory assumes that they also induce costs, leading to trade-offs between immunity and other fitness components. However, whether substantial fitness costs are associated with immune systems in the wild is debatable, as numerous factors may influence the costs and benefits associated with immune activation. Here, we explore the survival cost of immune deployment in postfledging birds. We injected Eurasian collared dove nestlings (Streptopelia decaocto) with antigens from Escherichia coli, and examined whether this immune challenge affected survival after fledging. To assess survival, birds we…

Construction de Modèles Prédictifs pour l'Analyse des Relations Oiseaux-Paysage

National audience; Cet article présente une comparaison de trois méthodes (Modèles Linéaires Généralisés, Réseaux de Neurones, Machines Vecteurs Supports) et de différentes combinaisons de prétraitements de données (filtrage, arrondi, analyse factorielle, sélection de paramètres). L'objectif de cette comparaison est de définir quel est le processus qui permet de construire le meilleur modèle prédictif, dans le cadre de la prédiction d'abondances d'espèces d'oiseaux à partir de variables décrivant le paysage. Nous comparerons les modèles grâce à l'erreur moyenne absolue et à l'information mutuelle. Cette comparaison a montré qu'aucune technique étudiée ne permet de construire des modèles pré…

Biodisponibilité et transfert d'éléments traces métalliques dans les réseaux trophiques terrestres.

National audience

Efficient unsupervised clustering for spatial bird population analysis along the Loire river

International audience; This paper focuses on application and comparison of Non Linear Dimensionality Reduction (NLDR) methods on natural high dimensional bird communities dataset along the Loire River (France). In this context, biologists usually use the well-known PCA in order to explain the upstream-downstream gradient.Unfortunately this method was unsuccessful on this kind of nonlinear dataset.The goal of this paper is to compare recent NLDR methods coupled with different data transformations in order to find out the best approach. Results show that Multiscale Jensen-Shannon Embedding (Ms JSE) outperform all over methods in this context.

Spatial dynamics of an invasive bird species assessed using robust design occupancy analysis: the case of the Eurasian collared dove ( Streptopelia decaocto ) in France

Aim  The study of the spatial dynamics of invasive species is a key issue in invasion ecology. While mathematical models are useful for predicting the extent of population expansions, they are not suitable for measuring and characterizing spatial patterns of invasion unless the probability of detection is homogeneous across the distribution range. Here, we apply recently developed statistical approaches incorporating detection uncertainty to characterize the spatial dynamics of an invasive bird species, the Eurasian collared dove (Streptopelia decaocto). Location  France. Methods  Data on presence/absence of doves were recorded from 1996 to 2004 over 1045 grid cells (28 × 20 km) covering th…

Environmental stress affects the expression of a carotenoid-based sexual trait in male zebra finches.

SUMMARY Abiotic factors including thermal stress are suggested to exert constrains on sexual ornaments through trade-offs between sexual displays and physiological functions related to self-maintenance. Given the health properties of carotenoid pigments, carotenoid-based ornaments offer a relevant context in which to investigate the effect of environmental stress, such as ambient temperature, on the production and maintenance of secondary sexual traits and, also, to explore the proximate mechanisms shaping their expression. In this study, we exposed male zebra finches (Taeniopygia guttata) to environmental stress by exposing them to two temperature regimes (6 and 26°C) over a 4 week period.…

Aging parasites produce offspring with poor fitness prospects.

Senescing individuals have poor survival prospects and low fecundity. They can also produce offspring with reduced survival and reproductive success. We tested the effect of parental age on the performance of descendants in the nematode Heligmosomoides polygyrus , an intestinal parasite of rodents. We found that offspring of senescing worms had reduced within-host survival and reduced egg shedding over the first month post-infection compared with offspring produced by young parents. These results suggest that declining offspring quality is a component of senescence in parasitic nematodes and might have evolutionary consequences for the optimal schedule of age-dependent investment into repr…

Validating graph-based connectivity models using independent presence/absence and genetic datasets.

Immune evasion, immunopathology and the regulation of the immune system.

21 pages; International audience; Costs and benefits of the immune response have attracted considerable attention in the last years among evolutionary biologists. Given the cost of parasitism, natural selection should favor individuals with the most effective immune defenses. Nevertheless, there exists huge variation in the expression of immune effectors among individuals. To explain this apparent paradox, it has been suggested that an over-reactive immune system might be too costly, both in terms of metabolic resources and risks of immune-mediated diseases, setting a limit to the investment into immune defenses. Here, we argue that this view neglects one important aspect of the interaction…

An experimental manipulation of life-history trajectories and resistance to oxidative stress

Optimal investment into life-history traits depends on the environmental conditions that organisms are likely to experience during their life. Evolutionary theory tells us that optimal investment in reproduction versus maintenance is likely to shape the pattern of age-associated decline in performance, also known as aging. The currency that is traded against different vital functions is, however, still debated. Here, we took advantage of a phenotypic manipulation of individual quality in early life to explore (1) long-term consequences on life-history trajectories, and (2) the possible physiological mechanism underlying the life-history adjustments. We manipulated phenotypic quality of a co…

Mixed Driven Refinement Design of Multidimensional Models based on Agglomerative Hierarchical Clustering

20 pages; International audience; Data warehouses (DW) and OLAP systems are business intelligence technologies allowing the on-line analysis of huge volume of data according to users' needs. The success of DW projects essentially depends on the design phase where functional requirements meet data sources (mixed design methodology) (Phipps and Davis, 2002). However, when dealing with complex applications existing design methodologies seem inefficient since decision-makers define functional requirements that cannot be deduced from data sources (data driven approach) and/or they have not sufficient application domain knowledge (user driven approach) (Sautot et al., 2014b). Therefore, in this p…

Assessing the Cost of Mounting an Immune Response

International audience; The evolution of parasite resistance has often been assumed to be governed by antagonistic selection pressures. Defense against pathogens, by mounting an immune response, confers evident benefits but may also incur costs, so that the optimal level of defense is expected to depend on the balance between benefits and costs. Although the benefits of immune surveillance are well known, estimates of costs are still equivocal. Here we studied the behavioral and physiological modifications associated with exposure to a onreplicating antigen (lipopolysaccharide [LPS] of Escherichia coli) in a passerine species, the house sparrow (Passer domesticus).We further investigated wh…

Inflammation and oxidative stress in vertebrate host–parasite systems

Innate, inflammation-based immunity is the first line of vertebrate defence against micro-organisms. Inflammation relies on a number of cellular and molecular effectors that can strike invading pathogens very shortly after the encounter between inflammatory cells and the intruder, but in a non-specific way. Owing to this non-specific response, inflammation can generate substantial costs for the host if the inflammatory response, and the associated oxygen-based damage, get out of control. This imposes strong selection pressure that acts to optimize two key features of the inflammatory response: the timing of activation and resolution (the process of downregulation of the response). In this p…

Impact of host nutritional status on infection dynamics and parasite virulence in a bird-malaria system.

10 pages; International audience; Host resources can drive the optimal parasite exploitation strategy by offering a good or a poor environment to pathogens. Hosts living in resource-rich habitats might offer a favourable environment to developing parasites because they provide a wealth of resources. However, hosts living in resource-rich habitats might afford a higher investment into costly immune defences providing an effective barrier against infection. Understanding how parasites can adapt to hosts living in habitats of different quality is a major challenge in the light of the current human-driven environmental changes. We studied the role of nutritional resources as a source of phenoty…

Increased susceptibility to oxidative stress as a proximate cost of reproduction

The neuropeptide calcitonin gene-related peptide differently modulates proliferation and differentiation of smooth muscle cells in culture depending on the cell type

Abstract Calcitonin gene-related peptide (CGRP) is a neuropeptide present around vasculature very early during development, when smooth muscle cells (SMC) are still proliferating and not yet totally differentiated. We investigated the effects of CGRP on proliferation and differentiation of SMC in culture; 10 −7 M CGRP added in the medium of cultured smooth muscle cells every 2 days did not significantly changed cells growth rate in 1% FCS. At the opposite, this treatment modulated proliferation of cells grown in 10% FCS medium. Two distinct populations of SMC with different growth rates were obtained from our primary cultures. SMC which proliferated slowly in the presence of 10% fetal calf …

An experimental test of the dose-dependent effect of carotenoids and immune activation on sexual signals and antioxidant activity.

Carotenoid‐based sexual traits are thought to be reliable indicators of male quality because they might be scarce and therefore might indicate the ability of males to gather high‐quality food and because they are involved in important physiological functions (as immune enhancers and antioxidants). We performed an experiment where male and female zebra finches (Taeniopygia guttata) were provided with increasing carotenoid doses in the drinking water during 4 weeks (bill color of this species is a carotenoid‐based sexual signal). Simultaneously, birds were split into two groups: one receiving weekly injections of Escherichia coli lipopolysaccharide in order to activate the immune system, the …

Do carotenoid-based sexual traits signal the availability of non-pigmentary antioxidants?

SUMMARY Carotenoid-based signals are thought to be indicators of male quality because they must be obtained from the diet and might thus indicate the ability of individuals to gather high-quality food. However, carotenoids are also known to have important physiological functions as immunoenhancers and antioxidants, and, as such, carotenoid-based sexual traits have also been suggested to reflect the health and antioxidant status of their bearers. This last idea is based on the hypothesis that carotenoids that are allocated to sexual signals are no longer available for the detoxification system. Recently, this hypothesis has been challenged on the grounds that the antioxidant activity is not …

Benefits of immune protection versus immunopathology costs: a synthesis from cytokine KO models.

5 pages; International audience; The inflammatory response can produce damage to host tissues and in several infectious diseases the most severe symptoms are due to immunopathology rather than a direct effect of pathogen multiplication. One hypothesis for the persistence of inflammatory damage posits that the benefits of protection towards infection outweigh the costs. We used data on knocked-out (KO) cytokine models [and the corresponding wild-type (WT) controls] to test this hypothesis. We computed differences in pathogen load and host survival between WT and KO and divided them by the WT values. Using this ratio provides an internal control for variation in pathogen species, host strain,…

Morphological variation and the recent evolution of wing length in the icterine Warbler: A case of unidirectional introgression?

Icterine Warbler Hippolais icterina and Melodious Warbler H. polyglotta are closely related species with parapatric breeding ranges. Their breeding ranges overlap only in a narrow zone in western Europe, where the Icterine Warbler population is presently declining and the Melodious Warbler population expanding. In eastern Burgundy (France), both species have bred in sympatry for at least forty years, but the Icterine Warbler started to decline in the mid-seventies. The two species differ in wing length and wing formula. Morphological variations of the wing were compared for the Icterine Warbler population in eastern Burgundy between 1965/76 and 1985/96. In the second period, wing length and…

A comparison of infestation patterns by Ixodes ticks in urban and rural populations of the Common Blackbird Turdus merula

Although spatial variation in the patterns of parasite infestations among host populations may have important ecological and epidemiological consequences, the causes underlying such variation are poorly known. In the context of a long-term study on the population biology of Common Blackbirds Turdus merula , we examined the prevalence and intensity of infestation by Ixodes ticks between birds living in rural vs. urban habitats. The overall prevalence of tick infestations was significantly higher in the rural habitat where 74% of individuals ( n = 130) were infested. This result contrasted markedly with the situation in the urban habitat where less than 2% of individuals ( n = 360) carried ti…

Species-specific song convergence in a moving hybrid zone between two passerines

Moving hybrid zones are receiving increasing attention. However, so far little is known about the proximate mechanisms underlying these movements. Signalling behaviour, by individuals engaged in interspecific sexual and aggressive interactions, may play a crucial role. In this study, we investigated song variation within a moving hybrid zone between two warblers, Hippolais polyglotta and H. icterina. In these species, song is involved in interspecific territoriality and, probably, in mixed pairings. We showed that allopatric populations of the two species are clearly acoustically differentiated. However, interspecific differences faded out in sympatry as a result of an overall pattern of co…

Increased susceptibility to oxidative damage as a cost of accelerated somatic growth in zebra finches.

1. Most animals do not grow at their maximal rate. This might appear puzzling because the early attainment of a large body size incurs several selective benefits, such as reduced risk of predation and earlier reproductive output. Several hypotheses have been suggested to explain this paradox. Among them, the cost due to high levels of oxidative stress, as the consequence of sustained metabolic activity during growth, has been put forward.

Spreading introgression in the wake of a moving contact zone

An increasing number of studies describe moving hybrid zones. This raises the issue of their actual frequency and emphasizes the need for methods that enable the detection of zone movements without historical records. Asymmetric introgression, usually considered as a signature of geographical shift, might be misleading when applied to mitochondrial or potentially non-neutral markers. We investigated mitochondrial and genomic introgression, using 30 AFLP derived markers, in a well-documented moving avian contact zone between two warblers. We found no instances of cross-species transmission of mitochondrial DNA but we detected nuclear introgression. Introgression levels were higher in the exp…

Ecologie évolutive de la réponse immunitaire.

36 pages

Male bill colour and age are associated with parental abilities and breeding performance in blackbirds

9 pages; International audience; In monogamous bird species, male parental investment may influence offspring fitness and females may gain advantages through mating with males providing extensive paternal care. However, paternal care is a benefit that can only be assessed indirectly because mate choice precedes paternal activities. Individual quality and age, both signalled by morphological characteristics, may reflect parental abilities. Because they may reflect individual foraging abilities, carotenoid-based colorations have been proposed to honestly signal parental quality. The blackbird (Turdus merula), a socially monogamous species, exhibits biparental care and males show bills that va…

Multidimensional Model Design using Data Mining: A Rapid Prototyping Methodology

[Departement_IRSTEA]Ecotechnologies [TR1_IRSTEA]MOTIVE; International audience; Designing and building a Data Warehouse (DW), and associated OLAP cubes, are long processes, during which decision-maker requirements play an important role. But decision-makers are not OLAP experts and can find it difficult to deal with the concepts behind DW and OLAP. To support DW design in this context, we propose: (i) a new rapid prototyping methodology, integrating two different DM algorithms, to define dimension hierarchies according to decision-maker knowledge; (ii) a complete UML Profile, to define a DW schema that integrates both the DM algorithms; (iii) a mapping process to transform multidimensional …

Immune activation rapidly mirrored in a secondary sexual trait.

A crucial assumption underlying most models of sexual selection is that sexual advertisements honestly reflect the phenotypic and/or genetic quality of their bearers ([1][1]). Here we show that experimental activation of the immune system is rapidly mirrored in the expression of a carotenoid-based

Early life infection and host senescence

IF 3.224 (2017); International audience; Advanced age is often associated with a chronic inflammatory status and inflammatory diseases. It has been suggested that exposure to infectious agents that stimulate the inflammatory response at early ages might have carry over effects in terms of accelerated senescence and increased mortality at late ages. However, not all pathogens and parasites have pro-inflammatory effects. In particular, parasitic nematodes have been shown to dampen the inflammatory response and to prevent or alleviate the symptoms of inflammatory diseases. We, therefore, tentatively predicted that early infection with a parasite that has anti-inflammatory properties might post…

Correlational selection on pro- and anti-inflammatory effectors.

9 pages; International audience; Parasites impose a permanent threat for hosts. As a consequence, immune defenses are important for host fitness. However, the immune response can also produce self-damage and impair host fitness if not properly regulated. Effectors that up- and downregulate the immune response should, therefore, evolve in concert, and be under the action of correlational selection. To address this issue, we assessed the shape of the selection operating on pro- and anti-inflammatory effectors following an inflammatory challenge in laboratory mice. We found that selection acts on the combination of these two traits as individuals that produced large amount of pro-inflammatory …

Lead concentrations in feathers and blood of common blackbirds (Turdus merula) and in earthworms inhabiting unpolluted and moderately polluted urban areas

9 pages; International audience; Despite the dramatic decrease of atmospheric lead (Pb) concentrations in urban areas of most industrialised countries, we hypothesised that urban common blackbirds (Turdus merula) may still be contaminated by Pb concentrations of toxicological concern due to transfer from soil through the food chain. We sampled blackbirds and earthworms, one of their main preys, in Besan?, a middle-size city of Eastern France (where atmospheric Pb concentrations decreased from 0.5 microg/m(3) in 1987 to nearly 0 in 2002) and in a rural reference site. Lead concentrations were determined in the tissues of the different functional groups of earthworms (anecic, epigeous and end…

Clutch-size variation in Western Palaearctic secondary hole-nesting passerine birds in relation to nest box design.

Møller, A.P. [et al.]

Testing hypotheses in evolutionary ecology with imperfect detection: capture-recapture structural equation modeling.

8 pages; International audience; Studying evolutionary mechanisms in natural populations often requires testing multifactorial scenarios of causality involving direct and indirect relationships among individual and environmental variables. It is also essential to account for the imperfect detection of individuals to provide unbiased demographic parameter estimates. To cope with these issues, we developed a new approach combining structural equation models with capture-recapture models (CR-SEM) that allows the investigation of competing hypotheses about individual and environmental variability observed in demographic parameters. We employ Markov chain Monte Carlo sampling in a Bayesian frame…

Does beak coloration of male blackbirds play a role in intra and/or intersexual selection?

In many bird species, males may show brightly coloured traits and variance in male mating success may be explained by female preference and/or competition between males favouring the most coloured males. Male beak coloration has been suggested to play an important role in the pairing pattern of European blackbirds. Here, we investigate female preference and male-male interactions in relation to male beak coloration in this species. We used a field experiment to measure female and male responses toward stuffed decoys showing either of two beak coloration representing the extremes of the natural variation from yellow to orange. Decoys were situated on the centre of plots and behaviours of mal…

Validation des modèles de connectivité issus de la théorie des graphes en utilisant des données génétiques

National audience; Modéliser la connectivité fonctionnelle des habitats est déterminant pour la conservation de la biodiversité. Les graphes paysagers, en modélisant des taches d’habitat reliées par des chemins de dispersion potentiels, permettent de quantifier la connectivité du paysage. Si cette approche semble prometteuse, sa validité écologique reste à démontrer. Tester sa validité implique de confronter des données de connectivité issues d’un graphe à des données reflétant la dispersion des individus, telles que des données génétiques. Dans cet objectif, nous avons modélisé la connectivité de l’habitat d’une espèce d’oiseau forestière, la Paruline caféiette (Setophaga plumbea), en Guad…

Life history adjustments to intestinal inflammation in a gut nematode.

ABSTRACT Many parasitic nematodes establish chronic infections. This implies a finely tuned interaction with the host immune response in order to avoid infection clearance. Although a number of immune interference mechanisms have been described in nematodes, how parasites adapt to the immune environment provided by their hosts remains largely unexplored. Here, we used the gastrointestinal nematode Heligmosomoides polygyrus to investigate the plasticity of life history traits and immunomodulatory mechanisms in response to intestinal inflammation. We adopted an experimental model of induced colitis and exposed worms to intestinal inflammation at two different developmental stages (larvae and …

Breeding strategy and morphological characters in an urban population of blackbirds, Turdus merula

Pairing patterns in monogamous birds are thought to be the consequence of mutual mate choice by males and females for characters linked to individual quality in the opposite sex. Although this may result in assortative pairing for a single character, there may be no simple match between male and female phenotype if the traits linked to individual quality are sex specific. For instance, in several passerine species, females tend to select males on the basis of territory quality or characters that may reflect health or vigour, whereas males benefit from pairing with females in good condition that breed early because early breeding increases reproductive success. We investigated pairing patter…

Stabilizing natural selection on the early expression of a secondary sexual trait in a passerine bird

Natural selection is a central tenet of evolutionary theory, yet the estimation of the direction and intensity of selection remains problematic. Here, we assess the strength of selection on the early expression of a secondary sexual ornament, bill colour, in male European blackbirds (Turdus merula) using 5 years of capture-mark-recapture (CMR) data. The best-fitting model consisted of a quadratic relationship between survival rate and bill colour, indicating stabilizing natural selection on the early expression of a secondary sexual trait. There was no evidence for sexual selection acting on bill colour in the first year. We suggest that the consideration of early selection and the adoption…

Disentangling the latitudinal and altitudinal shifts in community composition induced by climate change: The case of riparian birds

11 pages; International audience; Aim: This study investigates whether, and how, the composition of riparian bird communities has been affected by climate warming and habitat change. Although these two forces act separately, their respective contributions are rarely examined. Moreover, while the response of a given community may be a function of latitude and altitude, most studies have focused on these gradients separately. Riparian ecosystems are an opportunity to investigate community change along latitudinal and elevational gradients.Location: France, three major rivers (the Doubs, the Allier and the Loire)Taxon: Birds.Methods: Drawing upon bird community monitoring data over a period of…

Validation of graph-based connectivity models using genetic data.

Modelling the functional connectivity of habitats is crucial for biodiversity conservation. By modelling potential dispersal paths among habitat patches, landscape graphs are often used to quantify landscape connectivity. While this approach seems promising, it often lacks biological validation. To ensure its ecological relevance, we assessed the ability of connectivity metrics calculated from landscape graphs to predict population genetic structure that closely reflects the dispersal of individuals, and thus functional connectivity. We modelled the habitat network of a forest bird species (Plumbeous warbler, Setophaga plumbea) in Guadeloupe using three graphs constructed either from expert…

Carotenoid trade-off between parasitic resistance and sexual display: an experimental study in the blackbird (Turdus merula).

Many parasites depress the expression of the carotenoid-based colour displays of their hosts, and it has been hypothesized that animals face a trade-off in carotenoid allocation between immune functions and ‘degree of ornamentation’. While numerous correlative studies suggest that parasite infection decreases the intensity of carotenoid-based colour displays, the existence of this trade-off has never been demonstrated experimentally in a host–parasite model. In this study, we used the blackbird ( Turdus merula ) and Isospora (an intestinal parasite) to assess whether this trade-off does indeed exist. Blackbirds were supplemented with carotenoids while simultaneously being exposed to parasi…

Maintenance of male reaction to the congeneric song in the Hippolais warbler hybrid zone

Both hybridization and competition may induce character shift on communication systems in hybrid zones, leading either to convergence or divergence. Melodious warblers Hippolais polyglotta and Icterine warblers H. icterina are interspecifically territorial and hybridize along a narrow contact zone. This spatial distribution is favoured by a reaction to the congeneric song. A character displacement, e.g. a decrease of the reaction, was expected within the hybrid zone because of reproduction costs lower than in allopatry. We investigated the pattern of variation of the interspecific reaction by comparing allopatric and sympatric populations. However, our data did not support the hypothesis ch…

Microevolutionary response of a gut nematode to intestinal inflammation.

7 pages; International audience; Parasitic helminths interfere with the immune response of their hosts to establish long-lasting, chronic infections. While favorable to the parasite, the capacity to dampen the immune response can also provide a benefit to the host in terms of reduced risk of immune disorders and immunopathology. The immunomodulatory role of nematodes has been exploited in clinical trials to treat a number of inflammatory and immune diseases. However, how parasites adapt to an inflammatory environment remains a poorly explored question. Here, we conducted a serial passage experiment where the gut nematode Heligmosomoides polygyrus was maintained for nine generations in mice …

Influence of landscape composition and diversity on contaminant flux in terrestrial food webs: a case study of trace metal transfer to European blackbirds Turdus merula.

13 pages; International audience; Although understanding the influence of the spatial arrangement of habitats and interacting communities on the processes of pollutant flux and impacts is critical for exposure and risk assessment, to date few studies have been devoted to this emergent topic. We tested the hypothesis that landscape composition and diversity affect the transfer of trace metals to vertebrates. Bioaccumulation of Cd and Pb in blood and feathers of European blackbirds Turdus merula (n=138) was studied over a smelter-impacted area (Northern France). Landscape composition (type and occurrence of the different habitats) and diversity (number of different habitat types and the propo…

Interspecific variation in the relationship between clutch size, laying date and intensity of urbanization in four species of hole-nesting birds

Marie Vaugoyeau [et al.]

The energetic cost of humoral immunity in the Collared Dove, Streptopelia decaocto : is the magnitude sufficient to force energy-based trade-offs?

International audience; 1. Energy saving is often suggested as the basis of a resource trade-off between immunocompetence and other fitness-relevant traits. This suggests that the energetic cost of an immune response is significant and sufficient to force trade-offs. To date, few studies have investigated the energetic cost of the humoral component of the immune system in birds and furthermore, existing results are contradictory. 2. We addressed this question through two experiments. In experiment 1, the basal metabolic rate (BMR) of Collared Doves, Streptopelia decaocto , challenged with sheep red blood cells (SRBC) was compared with the BMR of control birds. The energetic cost of immunity…

Age reduces resistance and tolerance in malaria-infected mice.

7 pages; International audience; Once infected, hosts can rely on two strategies to cope with parasites: fight them (resist the infection) or minimize the damage they induce (tolerate the infection). While there is evidence that aging reduces resistance, how tolerance varies as hosts become old has been barely studied. Here, we used a rodent malaria parasite (Plasmodium yoelii) to investigate whether 2- and 12-month old house mice differ in their capacity to resist and tolerate the infection. We found that 12-month old mice harbored higher parasitemia, showing that age reduces resistance to malaria. Infection-induced deterioration of host health was assessed using red blood cell and body ma…

Dimension enrichment with factual data during the design of multidimensional models: application to bird biodiversity

20 pages; International audience; Data warehouses (DW) and OLAP systems are technologies allowing the on-line analysis of huge volume of data according to decision-makers’ needs. Designing DW involves taking into account functional requirements and data sources (mixed design methodology) [1]. But, for complex applications, existing automatic design methodologies seem inefficient. In some cases, decision-makers need querying, as a dimension, data which have been defined as facts by actual automatic mixed approachs. Therefore, in this paper, we offer a new mixed refinement methodology relevant to constellation multidimensional schema. The proposed methodolgy allows to decision-makers to enric…

Morphology and genetics reveal an intriguing pattern of differentiation at a very small geographic scale in a bird species, the forest thrush Turdus lherminieri.

12 pages; International audience; Mobile organisms are expected to show population differentiation only over fairly large geographical distances. However, there is growing evidence of discrepancy between dispersal potential and realized gene flow. Here we report an intriguing pattern of differentiation at a very small spatial scale in the forest thrush (Turdus lherminieri), a bird species endemic to the Lesser Antilles. Analysis of 331 individuals from 17 sampling sites distributed over three islands revealed a clear morphological and genetic differentiation between these islands isolated by 40-50 km. More surprisingly, we found that the phenotypic divergence between the two geographic zone…

Sensitive measure of prevalence and parasitaemia of haemosporidia from European blackbird (Turdus merula) populations: value of PCR-RFLP and quantitative PCR.

8 pages; International audience; Haemosporidian parasites are common in birds in which they act as an important selective pressure. While most studies so far have focused on the effect of their prevalence on host life-history traits, no study has measured the effect of parasitaemia. We developed molecular methods to detect, identify and quantify haemosporidia in 2 natural populations of the Blackbird Turdus merula. Three different parasite genotypes were found - 1 Haemoproteus and 2 Plasmodium. A PCR-RFLP screening revealed that only approximately 3% of blackbirds were free of parasites, compared to the 34% of uninfected birds estimated by blood smear screening. A quantitative PCR (q-PCR) a…

Disentangling the effect of host genetics and gut microbiota on resistance to an intestinal parasite

11 pages; International audience; Resistance to infection is a multifactorial trait, and recent work has suggested that the gut microbiota can also contribute to resistance. Here, we performed a fecal microbiota transplant to disentangle the contribution of the gut microbiota and host genetics as drivers of resistance to the intestinal nematode Heligmosomoides polygyrus. We transplanted the microbiota of a strain of mice (SJL), resistant to H. polygyrus, into a susceptible strain (CBA) and vice-versa. We predicted that if the microbiota shapes resistance to H. polygyrus, the FMT should reverse the pattern of resistance between the two host strains. The two host strains had different microbi…

Bill colour and immunocompetence in the European blackbird

The level of expression of secondary sexual characters has been suggested to signal male ability to resist parasitic infestations. To test this idea, several studies have examined the link between sexual signals and immunocompetence in birds. However, most of them have used only a single aspect of immune response to evaluate immunocompetence. We investigated the relation between bill colour and immunocompetence in captive male European blackbirds, Turdus merula, during the breeding season by assessing both cell-mediated and humoral components of the immune system. The blackbird is a sexually dimorphic species with bill colour varying from yellow to orange in males. Humoral immunity was asse…

Plastic and micro-evolutionary responses of a nematode to the host immune environment

9 pages; International audience; Parasitic organisms have to cope with the defences deployed by their hosts and this can be achieved adopting immune evasion strategies or optimal life history traits according to the prevailing pattern of immune-mediated mortality. Parasites often encounter variable immune environments both within and between hosts, promoting the evolution of plastic strategies instead of fixed responses. Here, we explored the plasticity and micro-evolutionary responses of immunomodulatory mechanisms and life history traits to the immune environment provided by the host, using the parasitic nematode Heligmosomoides polygyrus. To test if the parasite responds plastically to t…

Age-dependent allocation of carotenoids to coloration versus antioxidant defences.

SUMMARYAging is commonly attributed to age-related changes in oxidative damage due to an increased production of reactive oxygen species (ROS) and a weakened efficacy of enzymatic antioxidants. These age-related changes might therefore modify the use of dietary antioxidants, including carotenoids. As carotenoids are closely associated with the expression of secondary sexual signals, the allocation of carotenoids to sexual signal versus antioxidant defences may vary with age. In this study, we explored how carotenoid-based ornament and antioxidant activity varied with age and how an inflammatory-induced oxidative burst affected ornament and antioxidant activity across a range of ages. Using …

Species-specific song convergence in a moving hybrid zone between two passerines

International audience; Moving hybrid zones are receiving increasing attention. However, so far little is known about the proximate mechanisms underlying these movements. Signalling behaviour, by individuals engaged in interspecific sexual and aggressive interactions, may play a crucial role. In this study, we investigated song variation within a moving hybrid zone between two warblers, Hippolais polyglotta and H. icterina . In these species, song is involved in interspecific territoriality and, probably, in mixed pairings. We showed that allopatric populations of the two species are clearly acoustically differentiated. However, interspecific differences faded out in sympatry as a result of…

Testosterone and oxidative stress: the oxidation handicap hypothesis

Secondary sexual traits (SST) are usually thought to have evolved as honest signals of individual quality during mate choice. Honesty of SST is guaranteed by the cost of producing/maintaining them. In males, the expression of many SST is testosterone-dependent. The immunocompetence handicap hypothesis has been proposed as a possible mechanism ensuring honesty of SST on the basis that testosterone, in addition to its effect on sexual signals, also has an immunosuppressive effect. The immunocompetence handicap hypothesis has received mixed support. However, the cost of testosterone-based signalling is not limited to immunosuppression and might involve other physiological functions such as the…

A Methodology and Tool for Rapid Prototyping of Data Warehouses Using Data Mining: Application to Birds Biodiversity

Data Warehouses (DWs) are large repositories of data aimed at supporting the decision-making process by enabling flexible and interactive analyses via OLAP systems. Rapid prototyping of DWs is necessary when OLAP applications are complex. Some work about the integration of Data Mining and OLAP systems has been done to enhance OLAP operators with mined indicators, and/or to define the DW schema. However, to best of our knowledge, prototyping methods for DWs do not support this kind of integration. Then, in this paper we present a new prototyping methodology for DWs, extending [3], where DM methods are used to define the DW schema. We validate our approach on a real data set concerning bird b…

Pair-bonding in birds and the active role of females: a critical review of the empirical evidence.

Over the recent years the role of females in maintaining or breaking the pair-bond in socially monogamous birds has received growing attention. Here, we review the overall evidence for a direct and predominant role of female behaviour in initiating or preventing divorce and its relevance for the understanding of both interspecific and intraspecific variation in divorce rate in monogamous bird species. The evidence is so far limited to a few species. We discuss the relevance of some alternative explanations and confounding factors. We conclude that the possible predominance of females in both initiating divorce or limiting its occurrence deserves further consideration. Future studies should …

Supplement 1. BUGS code to implement the CR-SEM approach.

File List BUGScodes.txt (md5: 065a902fc724b73a8db114ad6c8875ae) Description BUGS codes for fitting SEM-CR models to the blackbird and blue tits data.

Data from: Correlational selection on pro- and anti-inflammatory effectors

Parasites impose a permanent threat for hosts. As a consequence, immune defenses are important for host fitness. However, the immune response can also produce self-damage and impair host fitness if not properly regulated. Effectors that up- and down-regulate the immune response should, therefore, evolve in concert, and be under the action of correlational selection. To address this issue, we assessed the shape of the selection operating on pro- and anti-inflammatory effectors following an inflammatory challenge in laboratory mice. We found that selection acts on the combination of these two traits as individuals that produced large amount of pro-inflammatory cytokines could achieve relative…

Data from: Impact of host nutritional status on infection dynamics and parasite virulence in a bird-malaria system

1. Host resources can drive the optimal parasite exploitation strategy by offering a good or a poor environment to pathogens. Hosts living in resource-rich habitats might offer a favourable environment to developing parasites because they provide a wealth of resources. However, hosts living in resource-rich habitats might afford a higher investment into costly immune defences providing an effective barrier against infection. Understanding how parasites can adapt to hosts living in habitats of different quality is a major challenge in the light of the current human-driven environmental changes. 2. We studied the role of nutritional resources as a source of phenotypic variation in host exploi…

Appendix A. Model selection.

Model selection.

Appendix B. Posterior predictive checks.

Posterior predictive checks.

Data from: Aging parasites produce offspring with poor fitness prospects

Senescing individuals have poor survival prospects and low fecundity. They can also produce offspring with reduced survival and reproductive success. We tested the effect of parental age on the performance of descendants in the nematode Heligmosomoides polygyrus, an intestinal parasite of rodents. We found that offspring of senescing worms had reduced within-host survival and reduced egg shedding over the first month post-infection compared with offspring produced by young parents. These results suggest that declining offspring quality is a component of senescence in parasitic nematodes and might have evolutionary consequences for the optimal schedule of age-dependent investment into reprod…

Appendix C. Posterior distributions of the CR-SEM parameters (conditional on the covariates being in the model).

Posterior distributions of the CR-SEM parameters (conditional on the covariates being in the model).

Data from: Early Plasmodium-induced inflammation does not accelerate aging in mice

Aging is associated with a decline of performance leading to reduced reproductive output and survival. While the antagonistic pleiotropy theory of aging has attracted considerable attention, the molecular/physiological functions underlying the early-life benefits/late-life costs paradigm remain elusive. We tested the hypothesis that while early activation of the inflammatory response confers benefits in terms of protection against infection, it also incurs costs in terms of reduced reproductive output at old age, and shortened longevity. We infected mice with the malaria parasite Plasmodium yoelii and increased the inflammatory response using an anti-IL-10 receptor antibody treatment. We qu…

Data from: Morphology and genetics reveal an intriguing pattern of differentiation at a very small geographic scale in a bird species, the forest thrush Turdus lherminieri

Mobile organisms are expected to show population differentiation only over fairly large geographical distances. However, there is growing evidence of discrepancy between dispersal potential and realized gene flow. Here we report an intriguing pattern of differentiation at a very small spatial scale in the forest thrush (Turdus lherminieri), a bird species endemic to the Lesser Antilles. Analysis of 331 individuals from 17 sampling sites distributed over three islands revealed a clear morphological and genetic differentiation between these islands isolated by 40–50 km. More surprisingly, we found that the phenotypic divergence between the two geographic zones of the island of Guadeloupe was …

Data from: Life-history adjustments to intestinal inflammation in a gut nematode

Many parasitic nematodes establish chronic infections. This implies a finely tuned interaction with the host immune response in order to avoid infection clearance. While a number of immune interference mechanisms have been described in nematodes, how parasites adapt to the immune environment provided by their hosts remains largely unexplored. Here, we used the gastrointestinal nematode Heligmosomoides polygyrus to investigate the plasticity of life history traits and immunomodulatory mechanisms in response to intestinal inflammation. We adopted an experimental model of induced colitis and exposed worms to intestinal inflammation at two different developmental stages (larvae and adults). We …