0000000000044526
AUTHOR
Maria Buscemi
I tessuti del dente
Expression and localization of MMP-9, MMP-2 and iNOS in some sample of human dental pulp orthodonticaly treated.
In Naive mice in vivo localization of WI specific CD8+CD28- induced cells
Expression of Cyclooxygenase-1 and Cyclooxygenase-2 in normal and pathological human oral mucosa
Abstract: Cyclooxigenase (COX) is the rate-limiting enzyme for the conversion of arachidonic acid (AA) to prostaglandins (PGs). Two isoforms of COX have been identified: COX-1 is constitutively expressed in many cells and is involved in cell homeostasis, angiogenesis and cell-cell signalling; COX-2 is not expressed in normal condition however it is strongly expressed in inflammation. The oral cavity is costantly exposed to physical and chemical trauma that could lead to mucosal reactions such as hyperplasia, dysplasia and cancer. Early diagnosis is the most important issue to address for a positive outcome of oral cancer; therefore it would be useful to identify molecular markers whose expr…
Cordon ombilical. Nouvelles sur l’acétylcholine
Introduction L’acetylcholine (Ach) fut la premiere substance impliquee dans la transmission synaptique. Elle est synthetisee a partir de l’enzyme acetyl-choline transferase (ChAT) et est degradee par l’acetyl-choline esterase (AchE). Il existe des anticorps specifiques diriges contre la ChAT et l’AchE qui ont permis de mettre en evidence la distribution de ce neurotransmetteur au niveau des systemes nerveux central et peripherique. Il faut rappeler que les neurones somato-moteurs, pre-ganglionnaires du tronc cerebral et de la moelle epiniere, les neurones post-ganglionnaires visceraux des ganglions parasympathiques et de quelques ganglions orthosympathiques sont cholinergiques. Ces donnees …
TP53 Mutation in exon 5 and S-Phase Fraction but not Mutations in Ras gene family and DNA-ploidy are Indipendent prognostic indicators in Laryngeal Squamous Cell Carcinoma
Role of Matrix Metalloproteinases 2,9 (MMP-2,-9), Orphanin FQ and Others Peptides in Vascular Tone of Human Umbilical Cord at Term
Umbilical cord is the structure that connects the fetus to the placenta. Umbilical cord at term is made of three vessels surrounded by the Wharton's Jelly, a mocous connective tissue rich in glycosaminoglycans, proteoglycans and different types of collagens and other microfibrils, which are responsible for its elasticity and its mechanical properties. Umbilical cord seems to lack any capillaries or lymphatics and umbilical vessels are unique in lacking any innervation of the vascular tone, and the expression of some enzymes responsible for the remodelling of the extracellular matrix. Trough immunihistochemistry we investigated the expression of matrix metallo-peptidase 2 and 9 (MMP-2, MMP-9…
Le botti di guajaco tra scienza ed esoterismo:TommasoCampailla, genius loci
Atrial Natriuretic Peptide presence in parotid gland of human fetus at 13th week of development and in adult man
Our immunohistochemical study shows that atrial natriuretic peptide (ANP) is present in both human fetal and adult parotid gland. In the fetuses ANP is localized in cell clusters, while in adult parotid gland, the ANP is seen only in the wall of intra- and extralobular ducts. The results suggest that ANP might play a role in the differentiation of the parotid gland similarly as in brain and that during fetal growth ANP may play a role in the regulating the secretion of primitive salivary fluid, even if acini are still not developed. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 1, 55-58)
Role of K-RAS and P53 Gene in Salivary Glands Tumorigenesis
MMP-2, MMP-9, and iNOS Expression in Human Dental Pulp Subjected to Orthodontic Traction
Abstract Objective: To test the hypothesis that some metalloproteinases (MMP-2, MMP-9) and inducible nitric oxide synthetase (iNOS) enzymes in dental pulp samples do not vary when subjected to orthodontic treatment. Materials and Methods: Human dental pulps were taken from male and female patients (N=10; age 10–14 years). A straight wire technique was used with nickel-titanium or steel archwires. The increase of pressure applied on teeth was gradual. Five patients were subjected to premolar extractions after 14 months of treatment and one after 24 months. Samples were Bouin-fixed, paraffin-embedded, and afterwards processed for immunohistochemistry using anti-MMP-2, anti-MMP-9, and anti-iNO…
I Mitocondri
Effects of Propolis in cancer cells
Psychopathia Criminalis, veemenza e disumanizzazione. Presenza italiana di Oskar Panizza
Multiple Supernumerary Teeth with no Other Associated Diseases or Syndromes:Case Report
Localisation immunohistochimique du facteur natriurique atriale du tissu muqueux mûr du Cordon Ombilical humain après naissance et dans la Pulpe Dentaire
The prognostic role of TP53 mutations in advanced laryngeal squamous cell carcinoma (LSCC).
HGF-Induced Branching in MDCK Cells is Dependent on the Activation of MEK1 and the Inhibition of GSK-3
Clinical Anatomy and information technology.
Expression of Nitric Oxide Synthase in Inflamed Gingival Tissue: Immunoistochemical and Image Analysis
Maxillary Bone Necrosis Following the Use of Formaldehyde-Containing Paste: Three Case Reports
Bovine seminal vesicles ANP, Oxytocin, immunohistochemical expression.
Expression of MMP-2 and MMP-9 in odontogenic myxoma in a child: report of a clinical case
Odontogenic myxoma (OM) is a benign, locally invasive, non-metastasizing neoplasm of the jaw bones. Despite the benign nature of these lesions, there is a high rate of recurrence and the current recommended therapy, depending on the size and behaviour of the lesion, can vary from curettage with peripheral ostectomy, segmental resection up to radical resections for more aggressive lesions. OM is a rare tumour which occurs predominantly in the third decade of life and it is rare in children. Matrix metalloproteinases (MMPs) are a family of extracellular endopeptidases responsible for the degradation and remodelling of extracellular matrix, they are known to be involved in the progression and …
Does the orthodontic treatment induces dental pulp cellular death? Caspase-3 and-9, HSP60 and TUNEL expression
Objective: To evaluate the hypothesis of human dental pulp cell death during orthodontic treatement (O.T) and the degree of apoptosis through the expression level of the proteins Caspase-3,-9, TUNEL and HSP60. Materials and methods: Human dental Pulp were coming from both male and female patients (N=20; age 10-14years). The technique used was the Straight Wire, which involves Nickel-Titanium or Steel archwires. The increase of pressure applied on teeth was gradual. Some patients were subjected to a premolar extraction after 3 months treatment, and others after 6 months. Samples were Bouin-fixed, paraffin-embedded and afterwards processed for immunohistochemistry using anti-caspase-3,-9 anti…
Laser Pressure Catapulting (LPC): Optimization LPC-System and Genotyping of Colorectal Carcinomas
Genotype analysis is becoming more and more useful in clinical practice, since specific mutations in tumors often correlate with prognosis and/or therapeutic response. Unfortunately, current molecular analytical techniques often require time-consuming and costly steps of analysis, thus making their routine clinical use difficult. Moreover, one of the most difficult problems arising during tumor research is that of their cell heterogeneity, which depends on their clear molecular heterogeneity. SSCP analysis discriminates by means of aberrant electrophoresis migration bands, mutated alleles which may represent as little as 15-20% of their total number. Nevertheless, in order to identify by se…
Immunohistochemical and Biomolecular Identification of Orphanin FQ, eNOS, Atrial natriuretic Factor and Oxytocin in Rat Seminal Vesicles
In previous studies performed on rodents, we detected the presence of adreno-cholinergic and peptidergic innervation in seminal vesicles and other organs of the male genital system, such as prostate and ductus deferens, in which we also investigated the expression of NOS and NADPH-diaphorase. During the present project we focused our attention on the expression of some peptides involved in local control of smooth muscle relaxation, contractility, vasodilatation and control of blood flow in rat seminal vesicles. We investigated, through immunohistochemistry and RT-PCR, the presence of four peptides: orphanin, eNOS, ANF and oxytocin. Immunohistochemistry was used to detect the presence of the…
Expression of Nitric Oxide Synthase in inflamed gingival tissue: immunohistochemical and image analysis
We have used 12 Epulis taken during surgical operation and as control, some normal gingival specimen. The aim was to clarify the relationship between the degree of inflammation and NO production in several cases of Epulis, exhibiting different degree of inflammatory process and verifying the presence of cells involved in NO production. From the immuhistochemical data in our possession we can conclude that NO is one of the factor which module the pathogenecity degree of Epulis.
Nouvelle utilisation du Collagène de Type I dans la Détermination du potentiel metastatique des Cellules Tumorales : Etude Morphologique et Biochimique
Nm-23-H1 expression does not predict clinical survival in colorectal cancer patients
The gene Nm23, which encodes for a nucleoside diphosphate kinase, has been defined as a metastasis-suppressor gene because of the inverse correlation between its expression and the metastatic capacity of the tumor cells. For colorectal cancer, however, the findings are equivocal. The aim of our study was to assess, in 160 patients undergoing surgery for colorectal cancer (CRC), the expression of the Nm23-H1 protein and to evaluate its possible associations with traditional clinicopathologic variables, with DNA-ploidy and proliferative activity (S-phase fraction, SPF), and with disease-free and overall survival of patients. Nm23-H1 expressions were evaluated on paraffin-embedded tissue by im…
Le Granulome éosinophile des maxillaires: une zone radiotransparente qui n’est pas toujours pathognomonique d’une véritable pathologie.
Modification of the peptides ANP and oxytocin in the rat hypothalamic paraventricular nucleus during the physical exercises.
Presence of ANP in internal carotid artery (ICA) with and without atherosclerotic lesions
Mandibulary Eosinophilic Granuloma: Case Series
Eosinophilic granuloma is a proliferation of Langerhans cells in the bones that most commonly involving the skeletal system. The disease generally affects children or young adults with predominance in males and it is characterized by a single or multiple skeletal lesions. This study describes a few cases of mandibular Eosinophilic Granuloma and the effects of surgical curettage of lesions
Le remaniement de la Matrice Métallo-Proteinase est impliquée dans l'Odontogènese? Etude du MMP-9
Regulation of odontogenic gene expression in dental stem cells
Italian Journal of Anatomy and Embryology, Vol 115, No 1/2 (Supplement) 2010
Expression of gelatinases (MMP-2, MMP-9) and cyclooxygenases (COX-1, COX-2) in some benign salivary gland tumors.
Salivary gland tumors, most of which are rare benign tumors, represent a histologically heterogenous group with the greatest diversity of morphological and cellular features. The aim of this study is to analyse the expression and possible interactions between gelatinases (MMP-2, MMP-9) and cyclooxygenases (COX-1, COX-2) in some benign salivary gland tumors. We investigated the expression of gelatinases and cyclooxigenases in control salivary gland, Pleomorphic adenoma and Warthin's tumor through immunohistochemistry and Reverse Transcription – Polymerase Chain Reaction (PCR). We identified the expression of both classes of enzyme in normal samples and in the two types of pathological sampl…
La consulenza genetica in oncologia: implicazioni biomolecolari e cliniche.
MEK1 Required for Invasive Growth of mIMCD3 renal Cells in a 3D Collagen Matrix
Branching morphogenesis is an essential process during kidney development: This process controls epithelialization of the metanephric mesenchyme during the induction of branching structures and in this way determines the number of nephrons. Various grow factors, such HGF, have been implicated in branching morphogenesis in renal cells, including mIMCD3 ( murine inner medullary collecting duct) and MDCK cells. Growth factors which induce branching morphogenesis in renal cells also activate the MEK1/ERK pathway. The authors analyzed the potential role that this pathway might play in branching morphogenesis. The authors show that PD98059, a specific inhibitor of MEK1,inhibits branching of mIMCD…
Effects of Sulpiride Treatment on the Hypothalamic Supraoptic and Paraventricular Nuclei
S2-P114 MAXILLARY BONE NECROSIS FOLLOWING THE USE OF FORMALEHYD-CONTAINING PASTE. THREE CASE REPORTS
Carcinoma ereditario dell'ovaio (Ereditary Ovarian Cancer)
The neurosecretion of oxytocin in the hypothalamic magnocellular nuclei of training rats
Preliminary observations on the immunohistochemical distribution of nNOS in the development of human foetus eye
Histochemical and ultrastructural remarks of the urinary tract in some mammals.
STAT proteins: From normal control of cellular events to tumorigenesis
Signal transducers and activators of transcription (STAT) proteins comprise a family of transcription factors latent in the cytoplasm that participate in normal cellular events, such as differentiation, proliferation, cell survival, apoptosis, and angiogenesis following cytokine, growth factor, and hormone signaling. STATs are activated by tyrosine phosphorylation, which is normally a transient and tightly regulates process. Nevertheless, several constitutively activated STATs have been observed in a wide number of human cancer cell lines and primary tumors, including blood malignancies and solid neoplasias. STATs can be divided into two groups according to their specific functions. One is …
Nadph Diaphorase Histochemical Presence in some Mammal's Penis
Intrabdominal and extrabdominal portion of penis (rat, guinea pig, hamster and rabbit) have been used for histochemical activity Nadph-diaphorase. Pharmacological and electrophysiological studies, both in vivo and in vitro, make us to belive that, in man, dog, rabbit and rat, the relaxation of vascular smooth muscle of the cavernous body of penis, that is responsible for erection, is produced by the activation of a Non-Adrenergic, Non-Cholinergic (NANC) nervous pathway whose mediator is a new neurotrasmitter: Nitric Oxide (NO). Our observations provide the morphohistochemical base for the localization of NADPH-diaphorase in the different constitutive structures of the penis in some mammalia…
Human Umbilical Cord at Term. Immunohistochemical expression of MMP-2, MMP-9, Orphanin F/Q and others Peptides involved in the regulation of vascular tone
TP 53, H-K-Ras, P16INK4A gene molecular analysis in salivary gland tumors.
Immunohistochemical and transcriptional expression of the matrix metalloproteinases MMP-2 and MMP-9 in normal and pathological human oral mucosa
The oral cavity is exposed to chronic or recurrent, physical and chemical trauma that could lead to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). The objective of this study is to investigate the expression and the possible changes of the two matrix metalloproteinases MMP-2 and MMP-9 in normal and pathological human oral mucosa samples. Normal oral mucosa samples and three different types of pathological conditions (hyperplasia, dysplasia and carcinoma) were used for this study. Immunohistochemical analysis was used to evaluate protein expression for the two enzymes, while Reverse Transcription ? Polymerase Chain Reaction (RT-PCR) was used to investigate gene expression. Image…
TP53, H-K-RAS, P16INK4A GENE MOLECULAR ANALYSIS IN SALIVARY GLAND TUMORS
Ildegarda: del corpo, dell'anima
Expression of remodelling enzymes (MMP2-MMP9) in samples of salivary gland's tumors
Immunohistochemical localization of MMP-9 and Orphanin in some sample of dental pulp.
CORDON OMBILICAL.NOUVELLES SUR L'ACETYLCHOLINE
Human umbilical cord expresses several vasoactive peptides involved in the local regulation of vascular tone: protein and gene expression of Orphanin, Oxytocin, ANP, eNOS and iNOS.
Full-term human umbilical cord contains three blood vessels: two arteries coiled around a vein and surrounded by Wharton’s jelly, a mucous tissue with few mesenchymal stromal cells and abundant extracellular matrix. Umbilical vessels lack innervations, thus endothelial cells must play a role in the control of blood flow. The aim of this study was to investigate in human umbilical cord the expression of five peptides that could be involved in the regulation of vascular tone: Orphanin FQ, Oxytocin, Atrial Natriuretic Peptide (ANP), endothelial Nitric Oxide Synthase (eNOS) and inducible Nitric Oxide Synthase (iNOS). The expression of these molecules in full-term human umbilical cord …
GIOVANNI GORGONE" THE NESTOR OF ANATOMISTS"
Expression of cytokeratins in human umbilical cord at term
Background. Cytokeratins are the proteins that costitute intermediate filaments. In the cytoplasm, keratin filaments extend from the nucleus to the plasma membrane. This link is important for the structural organization of the cytoplasm and for many cellular processes such as mitosis, cell movement, cell differentiation and adhesion. The pattern of expression of cytokeratins is frequently organ or tissue specific and depends mainly on the type of epithelium, the level of differentiation and the stage of development. The expression of cytokeratins in umbilical cord has been investigated by many groups, but these previous studies gave only fragmentary informations: each single study focused o…
IL GRANULOMA CENTRALE A CELLULE GIGANTI DEI MASCELLARI
Central giant cell granuloma ( CGCG ), is a fibro-osseous lesion. The majority of lesions are observed in the mandible and mainly in children and young adults, more often in females than in males. This lesion, which the pathogenesis still remains obscure, appears as radiolucency, well or ill-defined, uni or multilocular with trabeculations coursing through the lesion. Histologic study shows giant cells, fibroblastic cells, and foci of hemorrhage and osteoid tissue. On the basis of clinical, radiological and histologic features, central giant cell granulomas can be classified as "non-aggressive" or "aggressive"; the histomorphometric analysis prove a significant increase in large giant cells…
DNA-Aneuploidy, High SPF and Specific P53 Mutations are Indipendent Prognostic Factors in Colorectal Cancer (CRC)
Le remaniement de la Matrice Metallo-Proteinase est impliquée dans l’Odontogènese? Etude sur le MMP-9
Magnetic Resonance Spectroscopy: principles and clinical applications. Experimental Medicine Reviews
Presence of ANP in the suprachiasmatic nucleus of the developing rat.
Localisation immunohistochemique du factuer natriuretique atriale du tissu muqueux mur du Cordon Ombelical humain après naissance et dans la Pulpe Dentaire.
Immunohistochemical and transcriptional expression of matrix metalloproteinases in full-term human umbilical cord and Human Umbilical Vein Endothelial Cells
Matrix metalloproteinases (MMPs) are extracellular zinc-dependent endopeptidases involved in the degradation and remodelling of extracellular matrix in physiological and pathological processes. MMPs also have a role on cell proliferation, migration, differentiation, angiogenesis and apoptosis. Umbilical cord is a special organ subjected to many changes during pre-natal life and whose cells can maintain a certain degree of plasticity also in post-natal period; for example recently they have been used as a source of stem cells. In this work we investigated the expression of MMPs in human umbilical cord and Human Umbilical Vein Endothelial Cells (HUVEC) though immunohistochemistry, RT-PCR and …
Functional Aspects of Adrenocholinergic and Peptidergic Innervation about Prostate and Seminal Vesicles in Mammals: A Review
This study was aimed to explain the functional role of mediation in the organs of the male reproductive system (prostate and seminal vesicles of rat and hamster), representing throught the visualization of adrenergic fibres (Falck Method), cholinergic fobres (Martinez-Rodriguez method) and by immunohistochemical methods to detect the P substance and the CGRP (PAP) in glandular structures. The adrenocholinergic and peptidergic mediators have a growing interest for their synergic action in the control of contractility, vasodilatation and most likely, of the secretory activity of the epithelium, probably expressed by andocrine-paracrine mechanisms.
Embriologia del collo e del mediastino superiore
ANP presence in the choroid plexus in the lateral ventricles in the development rat
COX- Expression in the inflamed gingival tissue:Immunohistochemistry,Cytochemistry and Biochemistry test.
Prostaglandins (PG) are chemical mediators for most physiological and pathological responses ,including cytoprotection of the gastric mucosa, blood coagulation,inflammation and pain (1,2,3). E2 Prostaglandins (PG2) play an important role of pathogenesis of periodontal diseases, since PG2 determines vasodilatation and increased vascular permeability, oedema and erythema.Therefore,it is not surprising to find high levels of PG2 in inflamed gingival tissues.Indeed ,in inflamed gingival tissue,PG2 levels of the Gingival Fluid (GCF) are known to be higher in periodontitis than in healthy gingiva.Moreover ,in different parodontitis the high PG2 level of the GCF is related to periodontal ligament …
The liver: three-dimensional geometrical models. About a new stereology.
Expression of Remodelling Enzymes MMPs in Hernia Formation
Cell differentiation markers in human umbilical cord; an immunohistochemical study
The full-term human umbilical cord is made of three blood vessels (two arteries and a vein) surrounded by the Wharton’s jelly, a soft connective tissue made of few mesenchymal stromal cells and an abundant extracellular matrix. Both endothelial and Wharton’s jelly cells are versatile in their differentiation potential; they have shown to be able to differentiate into several cell lineages. Since the differentiation potential of these cells is very wide, we thought it could be interesting to investigate the expression of several cell differentiation markers in the different regions of human umbilical cord. Immunohistochemistry showed the expression of E-cadherin and cytokeratins in the amnio…
Vent'anni di storia sullo studio immunoistochimico delle polpe dentali di mammiferi Twenty years'history of immunohistochemical mammals dental pulp studies
Orphanin FQ and motility in seminal vesicles
Expression of matrix metalloproteinases in full-term human umbilical cord and Human Umbilical Vein Endothelial Cells.
Background. Matrix metalloproteinases (MMPs) are extracellular zinc-dependent endopeptidases involved in breakdown and remodelling of extracellular matrix in physiological and pathological processes. MMPs have also a role on cell proliferation, migration, differentiation, angiogenesis and apoptosis. Umbilical cord is a special organ subjected to many changes during pre-natal life and whose cells can maintain a certain degree of plasticity also in post-natal period; for example recently they have been used as a source of stem cells. For these reasons we believe it is interesting to investigate the expression of some remodelling enzymes in this tissue. Human Umbilical Vein Endothelial cells (…