0000000000064097

AUTHOR

Leonarda Troiano

showing 3 related works from this author

MMP-7 promoter polymorphisms do not influence CD4+ recovery and changes in plasma viral load during antiretroviral therapy for HIV-1 infection.

2005

Summary Matrix metalloproteinase-7 (MMP-7) generates soluble Fas Ligand (FasL), which is involved in the apoptotic loss of CD4+ T cells during HIV infection. We evaluated whether two polymorphisms in MMP-7 promoter could influence CD4+ recover in response to antiretroviral therapy, and found that these polymorphisms are ineffective.

AdultCD4-Positive T-LymphocytesMaleImmunologyHuman immunodeficiency virus (HIV)HIV InfectionsMatrix metalloproteinasemedicine.disease_causeMMP-7; Fas ligand; CD4T cells; HIV infectionFas ligandPlasma viral loadGeneticsHumansMedicineMolecular BiologyGenetics (clinical)Polymorphism Geneticbusiness.industryMetalloendopeptidasesGeneral MedicineMiddle AgedViral LoadAntiretroviral therapySoluble fas ligandCD4 Lymphocyte CountAnti-Retroviral AgentsApoptosisMatrix Metalloproteinase 7ImmunologyHIV-1business
researchProduct

Characterization of cells with different mitochondrial membrane potential during apoptosis.

2005

Background Until now, the simultaneous analysis of several parameters during apoptosis, including DNA content and mitochondrial membrane potential (ΔΨ), has not been possible because of the spectral characteristics of the commonly used dyes. Using polychromatic flow cytometry based upon multiple laser and UV lamp excitation, we have characterized cells with different ΔΨ during apoptosis. Methods U937 cells were treated with the flavonoid quercetin (Qu) and stained with JC-1 to detect ΔΨ, propidium iodide (PI) for cell viability, Hoechst 33342 for DNA content, Annexin V conjugated with Alexa Fluor-647 for detection of phosphatidilserine (PS) exposure, marker of early apoptosis, or Mitotracke…

Programmed cell deathHistologyCell Membrane PermeabilityCell Survivalpolychromatic flow cytometry • mitochondrial membrane potential • apoptosis • JC-1 • propidium iodide • Hoechst • Annexin-VPopulationApoptosisHL-60 CellsDNA FragmentationPhosphatidylserinesBiologyPathology and Forensic MedicineFlow cytometryMembrane Potentialschemistry.chemical_compoundAnnexinCell Line TumormedicineHumansViability assayPropidium iodideeducationFluorescent Dyeseducation.field_of_studymedicine.diagnostic_testDaunorubicinCell BiologyDNAIntracellular MembranesU937 CellsCarbocyaninesFlow CytometryMolecular biologyMitochondriachemistryApoptosisCell cultureDoxorubicinLeukocytes MononuclearBenzimidazolesQuercetinCytometry. Part A : the journal of the International Society for Analytical Cytology
researchProduct

Mitochondrial alterations and tendency to apoptosis in peripheral blood cells from children with Down syndrome

2006

Different types of cells from subjects with Down syndrome (DS) have an increased susceptibility to cell death. We have studied apoptosis and mitochondrial (mt) membrane potential (DeltaPsi(m)) in peripheral blood mononuclear cells (PBMC) from DS children and age-matched healthy donors after in vitro treatment with apoptogenic molecules, along with mtDNA content. We found that PBMC from DS and healthy controls had a similar tendency to undergo apoptosis and a similar amount of mtDNA. However, in cells from DS subjects, mitochondria showed a higher loss of DeltaPsi(m), underlying the presence of an increasing susceptibility of these organelles to damaging agents.

MaleProgrammed cell deathMitochondrial DNADown syndromemedicine.medical_specialtyAdolescentDown syndromeBiophysicsApoptosisMitochondrionBiologyIn Vitro TechniquesBiochemistryPeripheral blood mononuclear cellDNA MitochondrialStructural BiologyInternal medicineOrganelleGeneticsmedicineHumansChildMolecular BiologyMembrane Potential MitochondrialmtDNAInfantCell Biologymedicine.diseaseIn vitroMitochondriaEndocrinologyApoptosisCase-Control StudiesChild PreschoolImmunologyLeukocytes MononuclearFemaleFEBS Letters
researchProduct