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RESEARCH PRODUCT

Mitochondrial alterations and tendency to apoptosis in peripheral blood cells from children with Down syndrome

Ornella BiagioniNicole PradaNicole PradaMauro MariottiUgo ConsoloL. CiacciMilena NasiMarcello PintiErika RoatRoberta FerraresiElisa NemesEnrico LugliLeonarda TroianoChiara GiovenzanaAndrea CossarizzaFiorella Balli

subject

MaleProgrammed cell deathMitochondrial DNADown syndromemedicine.medical_specialtyAdolescentDown syndromeBiophysicsApoptosisMitochondrionBiologyIn Vitro TechniquesBiochemistryPeripheral blood mononuclear cellDNA MitochondrialStructural BiologyInternal medicineOrganelleGeneticsmedicineHumansChildMolecular BiologyMembrane Potential MitochondrialmtDNAInfantCell Biologymedicine.diseaseIn vitroMitochondriaEndocrinologyApoptosisCase-Control StudiesChild PreschoolImmunologyLeukocytes MononuclearFemale

description

Different types of cells from subjects with Down syndrome (DS) have an increased susceptibility to cell death. We have studied apoptosis and mitochondrial (mt) membrane potential (DeltaPsi(m)) in peripheral blood mononuclear cells (PBMC) from DS children and age-matched healthy donors after in vitro treatment with apoptogenic molecules, along with mtDNA content. We found that PBMC from DS and healthy controls had a similar tendency to undergo apoptosis and a similar amount of mtDNA. However, in cells from DS subjects, mitochondria showed a higher loss of DeltaPsi(m), underlying the presence of an increasing susceptibility of these organelles to damaging agents.

yearjournalcountryeditionlanguage
2006-12-05FEBS Letters
10.1016/j.febslet.2006.12.058http://dx.doi.org/10.1016/j.febslet.2006.12.058