Predation of Typhlodromus longilaterus Athias-Henriot (Parasitiformes, Phytoseiidae) females on eggs and juveniles of the tetranychid mites Tetranychus urticae (Koch) and Panonychus citri (McGregor) (Acariformes, Tetranychidae)

Typhlodromus longilaterus is a generalist phytoseiid mite described by Athias-Henriot in 1957 and commonly found in Israel on spontaneous herbaceous plants. As very little is known about this species, the present study reports preliminary results on the predation capacity of phytoseiid females on eggs and juveniles of two tetranychid mites very common in the Mediterranean area, Tetranychus urticae and Panonychus citri. After 24 hours the phytoseiid females preyed on 15% of offered eggs of both tetranychids, while the prey ratio was higher on T. urticae eggs after this period (33.4% and 33.5% vs 20.8% and 16.2% after 2 and 3 days for T. urticae and P. citri respectively). The phytoseiid show…

<p class="Body"><strong>Biological parameters of <em>Neoseiulus longilaterus</em> (Athias-Henriot) (Parasitiformes, Phytoseiidae) fed on prey and pollen in laboratory conditions</strong></p>

Tetranychus urticae is the most important pest on different crops worldwide. Search for endemic phytoseiid mites co-occurring with the above pest, which might be good candidates as biocontrol agents, represents the policy followed by most scientists in recent years. The aim of the present work was to assess the various biological and demographic parameters of Neoseiulus longilaterus, associated with T. urticae on weeds, in laboratory conditions using the latter tetranychid and pollen of Oxalis pes-caprae as food. The results showed a better performance of the predator on the prey as regards the postembryonic development (7.63 and 8.18 days for prey and pollen respectively) and the demograph…

Bioactivity of Carlina acaulis essential oil and its main component to the olive fruit fly, Bactrocera oleae: ingestion toxicity, electrophysiological and behavioural insights

In recent years, botanical insecticides based on essential oils (EOs), or their main components, have received much attention as promising tools for Integrated Pest Management, due to their environmental safety and low side effects on non-target organisms. In this study, Carlina acaulis EO and its main component, carlina oxide, recently proven to be effective larvicidal and adulticidal agents against some insect pests, were analyzed for their toxicity, electroantennographic (EAG), and behavioral responses to adult olive fruit fly (Bactrocera oleae). The C. acaulis EO was more toxic to the tephritid than the carlina oxide, and both were more toxic to the same insect pest than EOs from other …

Cultivable microorganisms associated with honeys of different geographical and botanical origin

In this study, the composition of the cultivable microbial populations of 38 nectar honey and honeydew honey samples of different botanical and geographical origin were assessed. After growth in specific media, various colonies with different appearance were isolated and purified before phenotypic (morphological, physiological and biochemical traits) and genotypic [randomly amplified polymorphic DNA (RAPD), repetitive DNA elements-PCR (rep-PCR) and restriction fragment length polymorphism (RFLP)] differentiation. The identification was carried out by 16S rRNA gene sequencing for bacteria and, in addition to RFLP, by sequencing the D1/D2 region of the 26S rRNA gene for yeasts and the 5.8S-IT…

Api e pronubi selvatici nella produzione di Coriandrum sativum L. (Famiglia Apiaceae) in Sicilia occidentale

Coriandrum sativum L. (Apiaceae - Umbelliferae) is an aromatic annual plant, subspontaneous in several Mediterranean countries. It is cultivated in various states of western Asia, North Africa and Europe. In Italy with the increment of immigration the request of coriandrum fresh state (grass cimicina) and powder to seeds are in increase. In Sicilian territory, for estimating the role of pollinators (wild pollinators and bees) in the increment of seeds production of Coriandrum sativum, a triennial surveying has been carried out, comparing three treatments: self-pollination, bees pollination and free pollination. The production of Coriandrum sativum obtained have been in the first year of 132…

First record ofTetranychus ludeniZacher (Acariformes: Tetranychidae) in Italy

Tetranychus ludeni Zacher is reported here for the first time in Italy. It was found on some cultivated and spontaneous plants, often associated with Tetranychus urticae Koch. Four phytoseiid mites, widely distributed in the Mediterranean countries, Phytoseiulus persimilis Athias-Henriot, Iphiseius degenerans Berlese, Typhlodromus (Anthoseius) kerkirae Swirski and Ragusa, and Typhlodromus (Anthoseius) transvaalensis Nesbitt, were found associated with T. ludeni In Sicily. The morphological characters for the identification of the four Italian Tetranychus species are also reported.

On the perception of leaf morphology and visible light by Tetranychus urticae Koch (Acariformes, Tetranychidae)

The two-spotted spider mite Tetranychus urticae is one of the most polyphagous and most damaging pests worldwide. The behaviour of the mite differs on its various host plants and this could influence crop defence strategies. We investigated the perception of leaf morphology by T. urticae in relation to the effects of visible light on the selection of sites on which females prefer to live and feed. Mite females chose the shaded surface when smooth and glabrous lemon leaves were offered as a surface for living and feeding, showing a photophobic behaviour. However, they clearly preferred the abaxial surface of bean leaves regardless of the presence or absence of visible light. As no difference…

Lethal and behavioural effects of a green insecticide against an invasive polyphagous fruit fly pest and its safety to mammals

Abstract Plant essential oil-based insecticides, with special reference to those that may be obtained from largely available biomasses, represent a valuable tool for Integrated Pest Management. However, the sublethal effects and the potential effects on aggressive insect traits of these green insecticides are understudied. Herein, the lethal and sub-lethal effects of the carlina oxide, constituting more than 97% of the whole Carlina acaulis (Asteraceae) root essential oil (EO), were determined against an invasive polyphagous tephritid pest, Ceratitis capitata (medfly). The carlina oxide was formulated in a mucilaginous solution containing carboxymethylcellulose sodium salt, sucrose, and hyd…

ANALISI DELLE POPOLAZIONI MICROBICHE RICORRENTI NEL MIELE

PHYTOSEIIDAE (ACARI: PARASITIFORMES) OF SICILY (ITALY): SPECIES DIVERSITY AFTER HALF A CENTURY OF SURVEYS

Gli acari fitoseiidi sono divenuti interesse di studio in Sicilia a partire dai primi anni '70. Ricerche costanti nel tempo hanno arricchito le conoscenze sulla fauna siciliana con la segnalazione di 48 specie valide, cinque delle quali nuove per la Scienza. Nel presente lavoro riassumiamo i dati raccolti in circa cinquant'anni, con l’aggiunta di 12 nuove segnalazioni per la fauna isolana. Typhlodromus (Anthoseius) cryptus Athias-Henriot   stata confermata la specie pi  frequente (39,4%), seguita da Typhlodromus (Typhlodromus) exhilaratus Ragusa (32,6%), Kampimodromus aberrans (Oudemans) (22,9%), Typhlodromus (Anthoseius) rhenanoides Athias-Henriot (19,4%) (18,9%). Alcune specie hanno mostr…

Carlina acaulis and Trachyspermum ammi essential oils formulated in protein baits are highly toxic and reduce aggressiveness in the medfly, Ceratitis capitata

Abstract Essential oil (EO)-based botanical insecticides are considered a promising option for Integrated Pest Management (IPM). However, the sublethal effects of EO-based insecticides are understudied. No information is available on the potential effects of EO exposure on insect aggressive traits. In this study, the toxicity of two EOs with documented efficacy on insect vectors and agricultural pests, and which were obtained from the roots of silver thistle (Carlina acaulis L., Asteraceae) and seeds (i.e. schizocarps) of ajwain (Trachyspermum ammi (L.) Sprague, Apiaceae), was evaluated against adults of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (medfly), a cosmopolitan fr…

Biological activity of Bacillus spp. evaluated on eggs and larvae of red palm weevil Rhynchophorus ferrugineus

This study was conducted to characterize the Bacillus populations associated with dead Rhynchophorus ferrugineus, to develop a biological control for the red palm weevil. Dead adult beetles, collected throughout Sicily, were used for isolating internal and external spore forming bacteria (SFB) microbiota. The isolates, preliminarily allotted to the Bacillaceae family, were tested at 4 concentrations (103 to 106 CFU/mL) for their ability to inhibit hatching of eggs of R. ferrugineus and were used at 106 CFU/mL to monitor their insecticidal activity against 10 day-old larvae. Total amounts of SFB measured outside the skeleton and in the inners part of the beetles were 5.59-6.94 and 5.17-7.05 …

Developing green insecticides to manage olive fruit flies? Ingestion toxicity of four essential oils in protein baits on Bactrocera oleae

Abstract Effective and eco-friendly plant-borne insecticides for developing lure and kill control tools against tephritid flies are scarce. Herein, the activity of four essential oils (EOs) obtained from two Apiaceae, Pimpinella anisum L. and Trachyspermum ammi (L.) Sprague, and two Lamiaceae, Thymbra spicata L. and Ocimum gratissimum L., was evaluated against the olive fruit fly, Bactrocera oleae (Rossi), a key pest of olive groves. The EO chemical composition was determined by gas chromatography coupled with mass spectrometry (GC-MS) analyses. The four EOs incorporated in protein baits were tested for ingestion toxicity on B. oleae adults, mimicking lure and kill assays. Results showed con…

Gelso in Sicilia: qualità, caratterizzazione e comportamento dei frutti in post-raccolta

Il gelso è una pianta arborea originaria dell'Asia e della Persia, introdotta in Italia dai Romani per le sue proprietà medicinali, oltre che per fini alimentari. Si distinguono due specie principali, comunemente denominati: gelso bianco e nero. I frutti, tipiche more di colore bianco violaceo o nero, sono molto apprezzati dall'uomo sin dall'antichità, sebbene vengano spesso collocati tra i frutti minori per la loro scarsa diffusione commerciale. Oggi, l'elevato interesse per le proprietà nutraceutichedi tali frutti spinge gli agricoltori ad una rivalutazione della gelsicoltura. Punto dolente della commercializzazione del gelso è la gestione post-raccolta a causa della brevissima shelf-life…