0000000000086013

AUTHOR

Rosa M. Collado

showing 3 related works from this author

The lipopolysaccharide O side chain of Vibrio vulnificus serogroup E is a virulence determinant for eels

1997

Vibrio vulnificus is a gram-negative bacterium capable of producing septicemic infections in eels and immunocompromised humans. Two biotypes are classically recognized, with the virulence for eels being specific to strains belonging to biotype 2, which constitutes a homogeneous lipopolysaccharide (LPS)-based O serogroup (which we have designated serogroup E). In the present study we demonstrated that the O side chain of this LPS determines the selective virulence of biotype 2 for eels: (i) biotype 1 strains (which do not belong to serogroup E) are destroyed by the bactericidal action of nonimmune eel serum (NIS) through activation of the alternative pathway of complement, (ii) biotype 2 str…

Lipopolysaccharidesendocrine systemanimal structuresLipopolysaccharideComplement Pathway AlternativeImmunologyMutantVirulenceVibrio vulnificusBiologyMicrobiologyMicrobiologychemistry.chemical_compoundPhagocytosisVibrionaceaeAnimalsVibrioEelsVirulenceO Antigensbiology.organism_classificationVirologyVibrioInfectious DiseaseschemistryAlternative complement pathwayImmunizationParasitologyBacteriaResearch Article
researchProduct

Effectiveness of different vaccine formulations against vibriosis caused by Vibrio vulnificus serovar E (biotype 2) in European eels Anguilla anguilla

2001

Vibriosis due to Vibrio vulnificus serovar E (biotype 2) is one of the main causes of mortality in European eels cultured in Europe. The main objective of this study was to develop a vaccine and a vaccination procedure against this pathogen. With this aim, we tested several vaccine formulations (inactivated whole-cells with and without toxoids‹inactivated extracellular products‹from capsulated and uncapsulated strains, attenuated live vaccines and purified lipopolysaccharide [LPS]) on eels maintained under controlled laboratory conditions using different delivery routes (injection and immersion). To study the immune response we estimated antibody titers and bactericidal/bacteriostatic activ…

SerotypeQuality ControlVibrio vulnificus serovar E ; Vibrio vulnificus biotype 2 ; Eel vaccines ; Vibrio vaccines ; Vaccination by injection ; Vaccination by prolonged immersionImmunization SecondaryVibrio vulnificus biotype 2Eel vaccinesVibrio vulnificusAquacultureAquatic ScienceBiologyAntibodies Viral:CIENCIAS DE LA VIDA [UNESCO]MicrobiologyFish DiseasesVaccination by injectionAntigenVibrionaceaeImmunityAntibody SpecificityUNESCO::CIENCIAS DE LA VIDAAnimalsUNESCO::CIENCIAS DE LA VIDA::Biología animal (Zoología)Vibrio vaccinesPathogenEcology Evolution Behavior and SystematicsVaccination by prolonged immersionSkinVibrio:CIENCIAS DE LA VIDA::Biología animal (Zoología) [UNESCO]VaccinationAntibody titerbiology.organism_classificationAnguillaVirologyVaccinationEuropeVibrio vulnificus serovar EVibrio InfectionsBacterial Vaccines
researchProduct

Comparative study of biological properties and electrophoretic characteristics of lipopolysaccharide from eel-virulent and eel-A virulent Vibrio vuln…

1999

ABSTRACT In Vibrio vulnificus , virulence for eels is associated with serovar E strains. In this study, we investigated some biological properties of purified lipopolysaccharides (LPSs) from serovar E and non-serovar E strains. Purified LPSs retained their O-polysaccharidic side chains and did not show any differences that could be related to host specificity, except for serological differences.

SerotypeLipopolysaccharidesErythrocytesLipopolysaccharideVirulenceVibrio vulnificusApplied Microbiology and BiotechnologyHemolysisMicrobiologychemistry.chemical_compoundFish DiseasesMiceVibrionaceaeVibrio InfectionsAnimalsVibrioEelsEcologybiologyVirulenceO Antigensbiology.organism_classificationVibrioRatscarbohydrates (lipids)Environmental and Public Health MicrobiologychemistryVibrio Infectionslipids (amino acids peptides and proteins)BacteriaFood ScienceBiotechnologyApplied and environmental microbiology
researchProduct