New tools to detect the quality of cultured european sea bass Dicentrarchus labrax (L.)

Sensitivity of cholinesterase activities in Ciona intestinalis ascidian to in vitro exposure to organophosphate pesticide azamethiphos

Developmental toxicity of Bisfenol A (BPA) in Ciona intestinalis (Ascidiacea, Urochordata)

A biochemical study of the effects of tributyltin on unfertilized eggs, embryos and larvae of the sea squirt Ciona intestinalis

We have used unfertilized eggs, embryos and larvae of the sea squirt Ciona intestinalis (Ascidiacea, Urochordata) to study the effects of tributyltin (TBT) on acetylcholine esterase (AChE) activity. Our results showed an increase of AChE enzyme activity levels after TBT exposure in both unfertilized eggs and embryos. The effects of TBT were more pronounced during the gastrula stage, while no differences between exposed larvae and controls were observed. A mechanism of TBT action on enzyme activity during ascidian embryonic development and the possibility of employing AChE in C. intestinalis as a biomarker of TBT exposure are suggested and discussed.

APOPTOSIS IN ASCIDIAN LARVAE AFTER TRIBUTYLTIN-CHLORIDE TREATMENT

Further data on expression of acetylcholinesterase during oogenesis and embryonic development of Ciona intestinalis and Ascidia malaca (Ascidiacea,Urochordata): a biochemical and histochemical study

Lipid and fatty acid variations inCiona intestinalis ovary after tri-n-butyltin(IV)chloride exposure

Reduction of total lipids (TL) content and significant variations of triglyceride (TG) and phospholipid (PL) fractions were observed as a consequence of exposure of Ciona intestinalis ovaries to tributyltin chloride (TBTCL) solutions. In particular, an evident TG decrease and a PL increase were observed, which probably provoked an increment in membrane fluidity, because of the high concentration of long chain fatty acids and, as a consequence, PL. This could be a cell adaptive standing mechanism towards the pollutants, as observed in Saccharomyces cerevisiae. Also the increase in the content of the polyunsaturated fatty acids (PUFA), important in the synthesis of compounds such as prostagla…

The compensatory regeneration of the operculum ofHydroides norvegica: Identification of the inhibiting substance

In the polychete Hydroides norvegica there are two opercula with different degrees of organization: one is completely developed and functional, the other, present only in rudimentary outline. The latter only begins to develop when the functional operculum is removed. It was observed that in the whole body of the Hydroides, but particularly in the functional operculum, there is a substance which inhibits the development of the rudimental operculum. This substance was purified in order to determine its chemical nature. From preparation of opercular homogenates we chemically separated numerous fractions, each of which was tested for inhibitory activity and chemical and physical properties. The…

Influence of environmental and farm conditions on Dicentrarchus labrax L.reared in three different fish farms

Effects of tributyltinchloride (TBT-Cl) on acetylcholinesterase (AChE) activity in unfertilized eggs and embryos of sea squirt Ciona intestinalis (Ascidiacea, Urochordata)

Further data on expression of acetylcholinesterase in female gamete, embryos and larva of Ciona intestinalis (Ascidiacea, Urochordata)

Sex hormone profiles in Ciona intestinalis (Ascidiacea Urochordata) and their modulation by Tributyltin (TBT)

Histochemical localisation of acetylcholinesterase activity in ovary and embryos of Ciona intestinalis and Ascidia malaca (Ascidiacea, Urochordata) after exposition to trybutyltin-chloride

A GC-MS method for hormone analysis and modulation of ovarian histology and steroidogenic genes by TBT and Bisphenol-A in Ciona intestinalis

Organometallic complexes with biological molecules. XIV. Biological activity of dialkyl and trialkyltin(IV) [meso-tetra(4-carboxy- phenyl)porphinate] derivatives

Molecules. XIV. Biological Activity of Dialkyl and Trialkyltin(IV) [Meso-tetra(4-carboxyphenyl)porphinate] Derivatives C. Mansueto, E. Puccia, F. Maggio, R. Di Stefano, T. Fiore, C. Pellerito, F. Triolo and L. Pellerito* Dipartimento di Biologia Animale, Universita di Palermo, Via Archirafi 18, 90123 Palermo, Italy Dipartimento di Chimica Inorganica, Universita di Palermo, Viale delle Scienze, Parco d’Orleans, 90128 Palermo, Italy

Cholinesterases in Ciona intestinalis (Ascidiacea, Urochordata): preliminary investigation of their potential as tools for monitoring marine pollution

V.Cangialosi,I.Corsi,S.Bonacci,C.Sensini, E.Puccia,S.Focardi and A.Mazzola Influence of environmental and farm conditions on Dicentrarchus labrax L.reared in three different fish farms

Azione dell'acido domoico sull'attività colinesterasica di Tapes philippinarum, Venus gallina, Glycimeri glycimeris (Lamellibranchia, Mollusca) e Ciona intestinalis (Ascidiacea, Urochordata)

Screening of Ovarian steroidogenic pathway in Ciona intestinalis and its modulation after Tributyltin exposure

In this study, we have identified several ovarian steroids in Ciona with high similarity to vertebrate steroids and showed that cholesterol, corticosterone, dehydroepiandrosterone, estrone, estradiol-17beta, testosterone, pregnenolone, progesterone, have identical molecular spectra with vertebrate steroids. In addition, we have studied the effects of an endocrine disruptor (tributyltin: TBT) on these sex hormones and their precursors, ovarian morphology, and gene expression of some key enzymes in steroidogenic pathway in the ovary of Ciona. Ovarian specimens were cultured in vitro using different concentrations of TBT (10{sup -5}, 10{sup -4} and 10{sup -3} M). Ethanol was used as solvent co…

Evidence for endocrine disruption of tributyltin chloride in ovary of Ciona intestinalis (Ascidiacea, Urochordata)

Organometallic complexes with biological molecules. XV. Effects of tributyltin(IV)chloride on enzyme activity, Ca2+, and biomolecule and synthesis inCiona intestinalis (Urochordata) ovary

Considerable attention has been given in recent years to the possibility that xenobiotics in the environment may affect reproduction in animals. In this study, the relative impact of tributyltin(IV) (TBT) chloride, one of the most toxic environmental pollutants, was investigated using Ciona intestinalis ovary as a model system. The pleiotropic effects of TBT exposure are concentration dependent and include a decrease of ATP levels, lipid content and nucleic acid content and synthesis. In contrast, a marked increase in calcium (Ca2+) and glucose content is observed. Furthermore, TBT alters enzymatic activity, inhibiting creatine kinase and stimulating alkaline phosphatase and cholinesterase …