0000000000109066
AUTHOR
Yanxia Hou
showing 7 related works from this author
Highly sensitive olfactory biosensors for the detection of volatile organic compounds by surface plasmon resonance imaging
2018
International audience; Nowadays, monitoring of volatile organic compounds (VOCs) is very important in various domains. In this work, we aimed to develop sensitive olfactory biosensors using odorant binding proteins (OBPs) as sensing materials. Three rat OBP3 derivatives with customized binding properties were designed and immobilized on the same chip for the detection of VOCs in solution by surface plasmon resonance imaging (SPRi). We demonstrated that the proteins kept their binding properties after the immobilization under optimized conditions. The obtained olfactory biosensors exhibited very low limits of detection in both concentration (200pM of beta-ionone) and in molecular weight of …
[Fri-P2-105] Deciphering the ligand binding properties of the mouse odorant-binding protein OBP5 from Mus musculus
2022
International audience; Odorant-binding proteins (OBPs) are abundant soluble proteins secreted in the nasal mucus of a variety of species which are believed to be involved in the transport of odorants towards olfactory receptors. In this study, we report the functional characterization of mouse OBP5 (mOBP5). mOBP5 was recombinantly expressed as a hexahistidine-tagged protein in bacteria and purified by metal affinity. Oligomeric state and secondary structure composition of mOBP5 was investigated using gel filtration and circular dichroism spectroscopy. Fluorescent experiments revealed that mOBP5 interacts with the fluorescent probe N-phenyl naphthylamine (NPN) with a micromolar affinity. Co…
Development of novel biomimetic sensor materials for optoelectronic nose applications
2019
International audience
Odorant-binding protein-based optoelectronic tongue and nose for sensing volatile organic compounds
2019
International audience; We developed an array of odorant-binding protein mutants with various binding properties. The same design is suitable for the detection and identification of volatile organic compounds (VOCs) both in the liquid phase and in the gas phase by surface plasmon resonance imaging. The obtained optoelectronic tongue is highly selective at low concentrations of VOCs with a low detection limit, but a narrow linear range. In comparison, the optoelectronic nose gives a much higher signal to noise ratio, but the discrimination of VOCs from different chemical classes requires kinetic data to get rid of non-specific signals. This work shows that these optoelectronic tongue and nos…
Development of a novel olfactory biosensor based on surface plasmon resonance imaging
2018
Monitoring volatile organic compounds (VOCs) is a key issue in many industrial domains, from food industry to public safety. Here, we propose a biomimetic alternative to traditional analytical methods such as gas chromatography and mass spectroscopy. Novel olfactory biosensor was developed using surface plasmon resonance imaging (SPRi) and odorant binding proteins (OBPs). OBPs are robust and can reversibly bind VOCs with a micromolar affinity. Herein, three mutant OBPs with tuned binding properties were immobilized on a chip in a microarray format to detect VOCs in liquid. We demonstrated that SPRi was efficient for the analysis of VOCs. The olfactory biosensor exhibited a low limit of dete…
Development of olfactory biosensors for the detection of small molecules by SPRi
2018
National audience
Surface plasmon resonance imaging for sensing volatile organic compounds: Biomimetic olfactory biosensors and optoelectronic nose
2018
National audience; Nowadays, there is a growing demand for the analysis of volatile organic compounds (VOCs) in various domains, including environment, quality control, and medical diagnostics. Traditional analytical methods, though accurate and reliable, require expensive equipment and are often time-consuming and laborious. On the other side, food & fragrance industries employ human sensory panels to evaluate the quality of an odour. However, panellists are expensive to train and employ and they can give biased results. To bridge the gap, we developed different sensor systems inspired by the human nose. Here, a biomimetic olfactory biosensor based on key-and-lock principle was designed us…