0000000000114941

AUTHOR

Antonio Pellı́n-perez

showing 3 related works from this author

CHARACTERIZATION OF A NEW RAT CELL LINE ESTABLISHED FROM 2′AAF-INDUCED COMBINED HEPATOCELLULAR CHOLANGIOCELLULAR CARCINOMA

2001

A rat cell line-nominated CC-62 derived from a combined hepatocellular and cholangiocellular carcinoma obtained by administration of 2-acetylaminofluorene to male Wistar rats, has been established. Using light and electron microscopy it was determined that morphologically the tumor consisted of a mixed population of hepatocytes and cholangiolar neoplastic cells, intermingled with small, undifferentiated oval-like cells. The CC-62 line has been maintained through 90 passages in culture adopting a paving stone arrangement. Doubling time at the 12th passage was 23 h. Immunostaining with a panel of antisera was performed to identify the cytological profiles of the cell line. There was no k-ras …

MaleCarcinoma HepatocellularC-MetTransplantation HeterologousPopulationCellMice NudeHistogenesisBiologyCholangiocarcinomaMicechemistry.chemical_compoundTumor Cells CulturedCarcinomamedicineAnimalsRats Wistareducationeducation.field_of_studyHepatocyte Growth FactorReverse Transcriptase Polymerase Chain ReactionLiver NeoplasmsDNA NeoplasmCell BiologyGeneral Medicine2-AcetylaminofluoreneProto-Oncogene Proteins c-metAneuploidymedicine.diseaseImmunohistochemistryMolecular biologyRatsTransplantationMicroscopy ElectronBile Ducts IntrahepaticGenes rasmedicine.anatomical_structureBile Duct NeoplasmschemistryCell cultureKaryotypingTumor Suppressor Protein p53ImmunostainingDevelopmental BiologyIn Vitro Cellular & Developmental Biology - Animal
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Cytogenetic and molecular findings related to rhabdomyosarcoma. An analysis of seven cases.

2003

Rhabdomyosarcoma (RMS) is the most common soft-tissue sarcoma in childhood. Histologically, it is subdivided histologically into two main subtypes: alveolar (ARMS) and embryonal (ERMS). ARMS is characterized by t(2;13)(q35;q14) or its variant t(1;13)(p36;q14), which fuse PAX3 and PAX7, respectively, with FKHR to produce chimeric genes. ERMS is frequently associated with loss of heterozygosity of 11p15.5. We investigated seven RMS (three ARMS and four ERMS) by means of cytogenetic, fluorescence in situ hybridization, and molecular analyses, including the study of the main genes implicated in the G1- to S-phase cell cycle transition, and correlated these studies with pathologic findings and c…

MaleCancer ResearchPAX3Genes mycLocus (genetics)Chimeric geneBiologyLoss of heterozygosityGene duplicationRhabdomyosarcomaGeneticsmedicineHumansPaired Box Transcription FactorsRhabdomyosarcomaChildMolecular BiologyPAX3 Transcription FactorIn Situ Hybridization FluorescenceChromosome AberrationsHomeodomain Proteinsmedicine.diagnostic_testForkhead Box Protein O1Hybridization probePAX7 Transcription FactorForkhead Transcription Factorsmedicine.diseaseMolecular biologyDNA-Binding ProteinsChild PreschoolFemaleFluorescence in situ hybridizationTranscription FactorsCancer genetics and cytogenetics
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Cytogenetic study of a spindle-cell rhabdomyosarcoma of the parotid gland.

1999

The cytogenetic analysis of a spindle-cell rhabdomyosarcoma of the parotid gland in a 6-year-old boy is reported. The tumor cells showed an abnormal karyotype with a hypotriploid modal chromosome number and clonal structural rearrangements affecting chromosomes 1, 8, 12, 21, and 22. The tumor karyotype was: 59, XY, -1, -3, -4, -5, -6, +8, +8, +del(8)(q22q24), -9, -10, del(12)(q13), -15, -16, -17, -18, der(21)t(12;21)(p11;p11), -22, der(22)t(1;22)(q12;p11).

Malecongenital hereditary and neonatal diseases and abnormalitiesCancer Researchmedicine.medical_specialtyPathologyTransplantation HeterologousMice NudeBiologyMyosinsDesminMiceRhabdomyosarcomaGeneticsmedicineAnimalsChromosomes HumanHumansVimentinRhabdomyosarcomaSpindle cell rhabdomyosarcomaChildMolecular BiologyChromosome AberrationsMyoglobinCytogeneticsKaryotypeModal Chromosome NumberAnatomymedicine.diseaseImmunohistochemistryActinsParotid glandParotid Neoplasmsmedicine.anatomical_structureKaryotypingImmunohistochemistrySarcomaNeoplasm TransplantationCancer genetics and cytogenetics
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