0000000000121133
AUTHOR
Béatrice Vincent
Caractères moisi-terreux des vins : origine et prévention
Note de synthèse du groupe national de travail ONIVINS - juillet 2005; National audience
Development of a qPCR assay for specific quantification of Botrytis cinerea on grapes
The aim of this study was to develop a system for rapid and accurate real-time quantitative PCR (qPCR) identification and quantification of Botrytis cinerea , one of the major pathogens present on grapes. The intergenic spacer (IGS) region of the nuclear ribosomal DNA was used to specifically detect and quantify B. cinerea . A standard curve was established to quantify this fungus. The qPCR reaction was based on the simultaneous detection of a specific IGS sequence and also contained an internal amplification control to compensate for variations in DNA extraction and the various compounds from grapes that inhibit PCR. In these conditions, the assay had high efficiency (97%), and the limit o…