0000000000132963
AUTHOR
K. L. Dramane
Docosahexaenoic acid modulates the expression of T-bet and GATA-3 transcription factors, independently of PPARα, through suppression of MAP kinase activation
The present study was conducted on CD4(+) T cells, isolated from wild type (WT) and PPARalpha(null) mice, in order to assess the mechanism of action of docosahexaenoic acid (DHA), an n-3 fatty acid, in the modulation of two transcription factors, i.e., T-bet and GATA-3, implicated in T-cell differentiation towards, respectively, T(H)1 and T(H)2 phenotype. The T-cells from PPARalpha(null) mice secreted higher IFN-gamma and lower IL-4 concentrations than WT T-cells. Furthermore, the deletion of PPARalpha gene in T-cells resulted in the upregulation of T-bet and downregulation of GATA-3 both at mRNA and protein levels. DHA exerted not only an inhibitory effect on T-cell proliferation, but also…
Modulation of adipokines and cytokines in gestational diabetes and macrosomia.
Abstract Context/Objective: Not much is known about the implication of adipokines and different cytokines in gestational diabetes mellitus (GDM) and macrosomia. The purpose of this study was to assess the profile of these hormones and cytokines in macrosomic babies, born to gestational diabetic women. Design/Subjects: A total of 59 women (age, 19–42 yr) suffering from GDM with their macrosomic babies (4.35 ± 0.06 kg) and 60 healthy age-matched pregnant women and their newborns (3.22 ± 0.08 kg) were selected. Methods: Serum adipokines (adiponectin and leptin) were quantified using an obesity-related multiple ELISA microarray kit. The concentrations of serum cytokines were determined by ELISA…
Modulations physiologiques et immunologiques au cours du diabète gestationnel et de la macrosomie : implication d’une programmation in utero et nouveau concept de « mémoire métabolique »
Le diabete gestationnel est un important facteur de risque pour l’obesite fœtale et neonatale, la macrosomie. Cette revue vise a faire le point sur l’etat des connaissances actuelles sur les plans metabolique et immunologique, en insistant sur l’implication du nouveau concept de la « memoire metabolique » creee au cours du diabete gestationnel, dans la survenue des maladies metaboliques chez l’enfant lorsqu’il atteint l’âge adulte. De nombreuses etudes ont montre que les anomalies du metabolisme des hydrates de carbone et des lipides, observees chez les bebes macrosomes nes de meres diabetiques, sont dues a l’hyperglycemie maternelle qui induit l’hyperinsulinemie fœtale. Nous avons developp…
Antioxidant status and circulating lipids are altered in human gestational diabetes and macrosomia.
Fetuses from mothers with gestational diabetes are at increased risk of developing neonatal macrosomia and oxidative stress. We investigated the modulation of antioxidant status and circulating lipids in gestational diabetic mothers and their macrosomic babies and in healthy age-matched pregnant women and their newborns. The serum antioxidant status was assessed by employing anti-radical resistance kit (KRL; Kirial International SA, Couternon, France) and determining levels of vitamin A, C, and E and the activity of superoxide dismutase (SOD). Circulating serum lipids were quantified, and lipid peroxidation was measured as the concentrations of serum thiobarbituric acid-reactive substances …