0000000000137476
AUTHOR
Anna Maria Flugy Pape'
ROLE OF EXOSOMES RELEASED BY CHRONIC MYLEOGENOUS LEUKEMIA CELLS IN THE MODULATION OF TUMOR MICROENVIROMENT
Role of interleukin 8 in exosome-mediated crosstalk between chronic myelogenous leukaemia cells and bone marrow stromal cells
Crosstalk between chronic myelogenous leukemia (CML)and bone marrow stromal cells: role of exosomes in the IL8- dependent signalling mediated by EGFR activation
Introduction: CML is a myeloproliferative disorder characterized by Bcr-Abl oncoprotein with a constitutive tyr-kinase activity. Exosomes (exo) shed by cancer cells potentially affect tumor-stroma interaction through the establishment of a bi-directional crosstalk. Interleukin-8 (IL8) is a proinflammatory chemokine that regulate proliferation and survival of cancer cells. We previously demonstrated that CML-derived exo modulate bone marrow microenvironment through the IL8 secretion from stromal cells. EGFR, as well as IL8, regulate cell proliferation and survival; it has been recently demonstrated that EGFR ligands can signal via exosomes shed by cancer cells. We hypothesized that the effec…
Analysis of cellular signalling and proteomic profiling in Imatinib-resistant chronic myeloid leukemia cells following treatment with carboxyamidotriazole
Effects of Parietaria judaica on human lung microvascular endothelial cells
Analisi del profilo dell'espressione genica delle cellule di carcinoma del colon umano T84 e T84SF: ruolo del pathway di AKT nel processo metastatico
carboxyamidotriazole-orotate inhibits the growth of imatinib resistant chronic myeloid leukaemia cells and modulates exosomes-stimulated angiogenesis
The Bcr/Abl kinase has been targeted for the treatment of chronic myelogenous leukaemia (CML) by imatinib mesylate. Although more common in solid tumors, increased microvessel density was also reported in chronic myelogenous leukaemia and was associated with a significant increase of angiogenic factors, suggesting that vascularity in haematologic malignancies is a controlled process and may play a role in the leukaemogenic process thus representing an alternative therapeutic target. Carboxyamidotriazole-orotate (CTO) is the orotate salt form of carboxyamidotriazole (CAI), an orally bioavailable signal transduction inhibitor that in vitro has been shown to possess antileukaemic activities (1…
Role of microRNAs shuttled by exosomes in the crosstalk between chronic myelogenous leukemia and endothelial cells
Effetti sinergici dell’azione antitumorale dell’epigallocatechina-3-gallato in combinazione con il carboxyamido-triazole, un’inibitore dei canali del calcio voltaggio-indipendenti.
CarboxyAmido-Triazole Orotate inhibits the growth of Imatinib-resistant chronic myeloid leukaemia cells and modulates exosomes-stimulated angiogenesis
Chronic myelogenous leukemia is a myeloproliferative disorder characterized by the t(9:22) (q34:q11) reciprocal chromosomal translocation, resulting in the expression of the chimeric Bcr–Abl oncoprotein with constitutive tyrosine kinase activity. Deregulated Bcr–Abl induces the hyperactivation of various signalling pathways that promote cell growth, suppress apoptosis and alter cell adhesion. Bcr-Abl has also been involved in VEGF-mediated angiogenesis in CML and evidence indicates that the formation of new vessels plays an important role in the development and progression of CML. Imatinib mesylate (IM) is a selective well tolerated inhibitor of the Bcr–Abl tyrosine kinase that has signific…
Chronic myeloid leukaemia-derived exosomes promote tumour growth through an autocrine mechanism
Exosomes released by k562 chronic myeloid leukemia cells promote endothelial cell tubular differentiation through uptake and cell-to-cell transfer
Exosomes, microvesicles of endocytic origin released by normal and tumor cells, play an important role in cell-to-cell ommunication. Angiogenesis has been shown to regulate progression of chronic myeloid leukemia (CML). The mechanism through which this happens has not been elucidated. We isolated and characterized exosomes from K562 CML cells and evaluated their effects on human umbilical endothelial cells (HUVECs). Fluorescent-labeled exosomes were nternalized by HUVECs during tubular differentiation on Matrigel. Exosome localization was perinuclear early in differentiation, moving peripherally in cells undergoing elongation and connection. Exosomes move within and between nanotubular stru…
Studio degli effetti di Imatinib Mesilato e CAI su linee cellulari di Leucemia mieloide cronica resistenti all'Imatinib mesilato
Chronic myeloid leukaemia- derived exosomes promote tumour growth and survival through an autocrine mechanism
Crosstalk between chronic myelogenous leukemia (CML) and bone marrow stromal cells: role of interleukin 8 and CML derived- exosomes
Chronic myelogenous leukemia (CML) is a myeloproliferative disorder characterized by the t(9:22) (q34:q11) reciprocal translocation, resulting in the expression of the chimeric Bcr–Abl oncoprotein with constitutive tyrosine kinase activity. Exosomes (Exo) are small vesicles of endosomal origin and of 40-100 nm diameter released by many cell types including cancer cells. Several data indicate that Exo play an important role in cell-to-cell communication and tumor-stroma interaction, thus potentially affecting cancer progression. It is well known that stromal microenvironment contributes to disease progression through the establishment of a bi-directional crosstalk with cancer cells. In the b…
Tumor microenvironment modulation by exosomes in chronic myelogenous leukemia
Exosomes are small vesicles of 40-100 nm diameter that are initially formed within the endosomal compartment and are secreted when a multivesicular body (MVB) fuses with the plasma membrane. These vesicles are released by many cell types including cancer cells and are considered messengers in intercellular communication. The exact function of exosomes in malignant cells has yet to be elucidated, but investigation has suggested roles in cell-to-cell communication, tumor-stroma interaction, and antigen presentation, thus potentially affecting cancer progression at different steps. Although production of exosomes by CML cells has been reported, little is known regarding the role of these vesic…
Comparative study of T84 and T84SF human colon carcinoma cells: in vitro and in vivo ultrastructural and functional characterization of cell culture and metastasis.
Role of E-selectin in the modulation of metastatic phenotype
Role of E-selectin in the modulation of metastatic phenotype
ESPRESSIONE DELLA E-SELECTINA IN CELLULE TUMORALI: POSSIBILE RUOLO NELLA MODULAZIONE DEL FENOTIPO METASTATICO?
Espressione della E-selectina in cellule tumorali: possibile ruolo nella modulazione del fenotipo metastatico
Effect of exosomes released from K562 CML cell line on gamma-delta T cells function
Exosomes mediate a paracrine interplay between Chronic Myelogenous Leukemia and stromal cells: a role for interleukin 8
Characterization of a subpopulation of T84 human colon cancer cells, after selection on activated endothelial cells.
In vitro antitumor effects of the cold-water extracts of Pleurotus eryngii var ferulae and Pleurotus nebrodensis on human colon cancer cells
For centuries, mushrooms have been used as folk medicines especially in Asian countries where their medicinal properties are well known. On the basis of numerous experimental evidences collected in the last decades, the immunomodulatory and anti-neoplastic properties of substances extracted from various species belonging to genera of edible mushrooms [Agaricus L., Auricularia Bull. ex Juss., Ganoderma P. Karst., Grifola Gray, Lentinus Fr., Schizophyllum Fr., Tremella Dill ex L., etc.] are extensively recognized also at scientific levels. Several works have demonstrate that anti-cancer property of these molecules is due to their ability to enhance immune system activity and/or to act directl…
Effect of Parietaria judaica pollen on human lung microvascular endothelial cells.
Carboxyamidotriazole-orotate inhibits the growth of Imatinib resistantchronic myeloid leukemia cells and modulates exosomes stimulated Angiogenesis
Chronic myeloid leukemia (CML) is characterized by the expression of Bcr–Abl oncoprotein with a constitutive tyrosine kinase that drives disease pathogenesis. Imatinib is the election therapy for CML, but some patients are resistant to this drug. Recently, attention is being focused on cell-cell communication that involves membrane vesicles called exosomes. A number of studies have described exosomes as new players in modulating the tumor microenvironment, promoting angiogenesis and tumor development; furthermore neovascularization is known to exert an important role in the progression of chronic myeloid leukaemia and may represent a valid alternative target for therapy. Little is known reg…
Exosomes released by chronic myelogenous leukemia cells modulate gamma-delta T cell activities
Role of exosomes released by chronic myelogenous leukemia cells in angiogenesis
Antitumor activity of water extract of pleurotus species growing on root residues against colon cancer cells.
Isolation and characterization of Citrus limon L. derived nanovesicles: potential use as antineoplastic agent
We isolated and characterized nanovesicles from edible Citrus limon with size and composition similar to mammalian-derived exosomes. Furthermore we show an in vitro and in vivo anti-proliferative and pro-apoptotic effect of these vesicles. This study opens to the possibility of using this natural plant-derived nanovesicles as antineoplastic agents.