0000000000138797

AUTHOR

Gilbert Turian

showing 6 related works from this author

Calcium, calmodulin-dependent protein phosphorylation in Neurospora crassa

1984

Abstract A calcium, calmodulin-dependent protein kinase activity has been partially purified by calmodulin-Sepharose affinity chromatography from the soluble fraction of Neurospora crassa . The phosphorylated peptide has an apparent molecular mass on SDS-polyacrylamide gel of 47 kDa. The apparent half maximal phosphorylation is obtained after 1.5 min at 30° C in the presence of calcium and calmodulin. The apparent half maximal activation of the phosphorylation is obtained at 1 μM calcium, and 0.1 or 0.2 μM calmodulin from bovine brain or Neurospora , respectively. The 32 P incorporation is enhanced about 10-fold by calmodulin.

[SDE] Environmental SciencesCalmodulin[SDV]Life Sciences [q-bio]Biophysicschemistry.chemical_elementCalciumBiochemistryNeurosporaProtein kinaseNeurospora crassa03 medical and health sciencesAffinity chromatographyCalmodulinStructural BiologyGenetics[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyProtein phosphorylation[SDV.BV] Life Sciences [q-bio]/Vegetal BiologyProtein kinase AMolecular BiologyComputingMilieux_MISCELLANEOUS030304 developmental biology0303 health sciencesbiologyNeurospora crassa030306 microbiologyCell Biologybiology.organism_classification[SDV] Life Sciences [q-bio]chemistryBiochemistry[SDE]Environmental Sciencesbiology.proteinPhosphorylationCalcium
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Actin in Allomyces arbuscula

1991

International audience; A 42 kDa protein was isolated by affinity chromatography on DNAse I-Sepharose from 24-h-old mycelia of Allomyces arbuscula. It was identified as actin by immunoblots with monoclonal antibody probe against a chicken gizzard actin. This fungal actin has a pI of 5.9 and separates into two spots on two-dimensional polyacrylamide gel electrophoresis, suggesting its dual nature. It can polymerize into 8-10 nm filaments visualized by electron microscopy.

[SDE] Environmental Sciencesmedicine.drug_classAllomyces[SDV]Life Sciences [q-bio]Plant Sciencemacromolecular substancesMonoclonal antibodylaw.invention03 medical and health sciencesAffinity chromatographylawGeneticsmedicine[SDV.BV]Life Sciences [q-bio]/Vegetal Biology[SDV.BV] Life Sciences [q-bio]/Vegetal BiologyPolyacrylamide gel electrophoresisEcology Evolution Behavior and SystematicsActin030304 developmental biologyGel electrophoresis0303 health sciencesbiology030306 microbiologybiology.organism_classificationMolecular biologyActina[SDV] Life Sciences [q-bio]Biochemistry[SDE]Environmental SciencesElectron microscopeBiotechnology
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Calmodulin-stimulated cyclic nucleotide phosphodiesterase from Neurospora crassa.

1983

International audience

[SDV] Life Sciences [q-bio][SDE] Environmental Sciences[SDV]Life Sciences [q-bio][SDE]Environmental Sciences[SDV.BV]Life Sciences [q-bio]/Vegetal Biology[SDV.BV] Life Sciences [q-bio]/Vegetal BiologyComputingMilieux_MISCELLANEOUS
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Immunopurification of highly specific antibodies against calmodulin from Neurospora crassa

1988

International audience

[SDV] Life Sciences [q-bio][SDE] Environmental Sciences[SDV]Life Sciences [q-bio][SDE]Environmental Sciences[SDV.BV]Life Sciences [q-bio]/Vegetal Biology[SDV.BV] Life Sciences [q-bio]/Vegetal BiologyComputingMilieux_MISCELLANEOUS
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A search for myosin in elongating hyphae of Neurospora crassa

1983

International audience

[SDV] Life Sciences [q-bio][SDE] Environmental Sciences[SDV]Life Sciences [q-bio][SDE]Environmental Sciences[SDV.BV]Life Sciences [q-bio]/Vegetal Biology[SDV.BV] Life Sciences [q-bio]/Vegetal BiologyComputingMilieux_MISCELLANEOUS
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Changed protein pattern during heat shock and conidiogenous shift in Neurospora crassa

1982

International audience

[SDV] Life Sciences [q-bio][SDV]Life Sciences [q-bio]Changed protein patternconidiogenous shift in Neurospora crassaheat shockComputingMilieux_MISCELLANEOUS
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