0000000000144535

AUTHOR

Venera Weinhardt

showing 3 related works from this author

Compact Cell Imaging Device (CoCID) provides insights into the cellular origins of viral infections

2021

The overall CoCID concept is centred on providing virologists with a next-generation imaging device, which, through increased penetration and depth of focus, as well as through high natural contrast and sensitivity to organelle density (including virus-related organelles), will produce higher-fidelity ultrastructural images of whole intact cells. These insights will, in turn, help increase our understanding of the links between the structural reorganisation of cells and the mechanisms of viral entry, replication, assembly, and egress in cells. CoCID will provide this valuable imaging capability in the form of a compact lab-scale device that will greatly improve the accessibility of soft X-r…

2019-20 coronavirus outbreakCoronavirus disease 2019 (COVID-19)viruksetSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)CelltutkimuslaitteetBiologymikroskopiawater windowinfektiot03 medical and health sciences0302 clinical medicineherpesvirussoft x-ray microscopymedicinehepatiitti C -virusElectrical and Electronic Engineeringherpesvirukset030304 developmental biology0303 health scienceshepatiitti E -virusSARS-CoV-2röntgensäteilySARS-CoV-2-virusHepatitis Cmedicine.diseaseHepatitis EVirologyAtomic and Molecular Physics and Optics3. Good healthElectronic Optical and Magnetic Materialsmedicine.anatomical_structurekuvantaminenfotoniikka030220 oncology & carcinogenesiscell structure imaginghepatiittiviruksetSoft x-ray microscopyhepatitis Ehepatitis Csolubiologia
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Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells.

2022

Upon infection, viruses hijack the cell machinery and remodel host cell structures to utilize them for viral proliferation. Since viruses are about a thousand times smaller than their host cells, imaging virus-host interactions at high spatial resolution is like looking for a needle in a haystack. Scouting gross cellular changes with fluorescent microscopy is only possible for well-established viruses, where fluorescent tagging is developed. Soft X-ray tomography (SXT) offers 3D imaging of entire cells without the need for chemical fixation or labeling. Here, we use full-rotation SXT to visualize entire human B cells infected by the herpes simplex virus 1 (HSV-1). We have mapped the temporo…

viruksetisäntäsolutBioengineeringmikroskopiainfektiotMicrobiologyX-ray tomography; soft X-rays; infection imaging; HSV-1; cell mapping; cryo imagingherpes simplex -virusCapsidsoft X-raystomografiaVirologyHumans2.2 Factors relating to the physical environment2.1 Biological and endogenous factorsAetiologyherpesviruksetTomographycryo imagingHerpesvirus 1herpesinfection imagingHSV-1solutInfectious Diseasesröntgenkuvauscell mappingX-RaySexually Transmitted InfectionsInfectionX-ray tomographysolubiologiaHuman
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Quantitative microscopy reveals stepwise alteration of chromatin structure during herpesvirus infection

2019

During lytic herpes simplex virus 1 (HSV-1) infection, the expansion of the viral replication compartments leads to an enrichment of the host chromatin in the peripheral nucleoplasm. We have shown previously that HSV-1 infection induces the formation of channels through the compacted peripheral chromatin. Here, we used three-dimensional confocal and expansion microscopy, soft X-ray tomography, electron microscopy, and random walk simulations to analyze the kinetics of host chromatin redistribution and capsid localization relative to their egress site at the nuclear envelope. Our data demonstrated a gradual increase in chromatin marginalization, and the kinetics of chromatin smoothening arou…

viruseslcsh:QR1-502Herpesvirus 1 HumanmikroskopiaVirus ReplicationinfektiotElectronMicrobiologylcsh:MicrobiologyArticleFluorescenceCell LineBiokemia solu- ja molekyylibiologia - Biochemistry cell and molecular biologyherpes simplex -virustumaChlorocebus aethiopsAnimalsHumansherpesviruksetVero CellsTomographyVirus ReleaseCell NucleusMicroscopyTomography X-RayHerpesvirus 1nuclear egressHerpesviridae InfectionsHSV-1ChromatinMicroscopy ElectronInfectious DiseasesMicroscopy FluorescencetumaegressKasvibiologia mikrobiologia virologia - Plant biology microbiology virologyX-RaykromatiiniSexually Transmitted InfectionschromatinInfectionHuman
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