Leaching of diuron and oryzalin in undisturbed lysimeters of three vineyard soils (Vosne-Romanée, 21)

La mobilité du diuron et de l'oryzalin a été étudiée en colonnes de sol non perturbé au laboratoire. Les sols étudiés sont trois sols viticoles de la région Bourgogne (Vosne-Romanée, 21) et ont été prélevés selon une topolithoséquence. Leurs teneurs en carbone organique sont respectivement de 1,2 %, 1,7 % et 2,2 % pour le sol brun calcaire de mi-pente (SBC MP), le sol brun calcaire de bas de pente (SBC BP) et la rendzine. Après une hauteur de pluie simulée de 845 mm, les percolats respectifs de la rendzine, du SBC MP et du SBC BP contiennent des quantités plus importantes de diuron (3,2 %, 11,8 % et 18,8 %), que d'oryzalin (0,2 %, 4,9 % et 3,7 % des quantités appliquées). Dans les colonnes …

Transfer of hexazinone and glyphosate through undisturbed soil columns in soils under Christmas tree cultivation.

Abstract Field studies monitoring pesticide pollution in the Morvan region (France) have revealed surface water contamination by some herbicides. The purpose of this study was to investigate in greater detail the transport of two herbicides, used in Christmas tree production in the Morvan, under controlled laboratory conditions. Thus, the leaching of hexazinone (3-cyclohexyl-6-dimethyl-amino-1-methyl-1,3,5-triazine-2,4 (1H,3H) dione) and glyphosate ( N -(phosphono-methyl-glycine)) through structured soil columns was studied using one loamy sand and two sandy loams from sites currently under Christmas tree cultivation in the Morvan. The three soils were cultivated sandy brunisol [Sound refer…

Assessing the transfer of pentachlorophenol through soil columns using 13[C]isotope

Abstract The transfer of organic pollutants was studied through soil columns using 13 [C]-labelled pentachlorophenol (PCP) as a model compound. The organic carbon content and the 13 [C]/ 12 [C] ratio were measured in two soil sections, 0–3 cm and 3–6 cm, and in percolated water using an Elemental Analyser coupled with a Magnetic Mass Sector. The mass balance of carbon was evaluated and the amount of PCP was calculated in each compartment of the soil–water systems. The results show that more than 80% of the PCP-derived 13 [C] remained in the upper layer of the soil column. Approximately 20% was transferred to the lower soil layer, and less than 1% was found in the water leachates. The 13 [C]…

Insecticide residues in cotton soils of Burkina Faso and effects of insecticides on fluctuating asymmetry in honey bees (Apis mellifera Linnaeus).

8 pages; International audience; Four insecticides (acetamiprid, cypermethrin, endosulfan and profenofos) are used quarterly in the cotton-growing areas of Burkina Faso, West Africa. These insecticides were investigated in soils collected from traditionally cultivated and new cotton areas. Also, the effects of insecticide exposure on the developmental instability of honey bees, Apis mellifera, were explored. In soil samples collected three months after insecticide treatments, endosulfan and profenofos concentrations varied in the range of 10-30μgkg(-1) in the traditionally cultivated zones and 10-80μgkg(-1) in the new cotton zones, indicating a pollution of agricultural lands. However, only…

The relationship between copper accumulated in vineyard calcareous soils and soil organic matter and iron

Summary The intensive use for over 100 years of copper sulphate (Bordeaux mixture) to fight mildew in vineyards has led to a substantial accumulation of copper (Cu) in surface soils. To assess the effects of such large concentrations, the surface soils of 10 Burgundy vineyards were sampled and analysed for total organic matter (carbon and nitrogen) and metal (copper and iron) contents. Physical (i.e. size fractionation) and chemical (sequential extraction) methods were used to determine the distribution of these elements. The most Cu-contaminated plots showed the largest accumulation of organic carbon and Cu in the coarse sand and fine sand fractions. Copper was strongly correlated with org…

Copper binding capacity of root exudates of cultivated plants and associated weeds

International audience; Cu binding to root exudates of two cultivated plants, wheat (Triticum aestivum) and rape (Brassica napus), and two weeds associated with wheat, dog daisy (Matricaria inodora) and cornflower (Centaurea cyanus), was studied in vitro under hydroponic and sterile conditions. Nutrient solutions were prepared with or without P. A MetPLATE microbiological test was used to assess the metal complexing capacity of root exudates. In the P-deficient solutions, no exudation was observed for any of the four plants; consequently, no Cu binding occurred. When P was present in the nutrient solutions, the plant exudates displayed differing abilities to complex Cu. No difference was de…

Leaching of glyphosate and AMPA under two soil management practices in Burgundy vineyards (Vosne-Romanée, 21-France)

Some drinking water reservoirs under the vineyards of Burgundy are contaminated with herbicides. Thus the effectiveness of alternative soil management practices, such as grass cover, for reducing the leaching of glyphosate and its metabolite, AMPA, through soils was studied. The leaching of both molecules was studied in structured soil columns under outdoor conditions for 1 year. The soil was managed under two vineyard soil practices: a chemically treated bare calcosol, and a vegetated calcosol. After 680 mm of rainfall, the vegetated calcosol leachates contained lower amounts of glyphosate and AMPA (0.02% and 0.03%, respectively) than the bare calcosol leachates (0.06% and 0.15%, respectiv…

Alachlor and Bentazone Losses from Subsurface Drainage of Two Soils

International audience; Atrazine (6-chloro-N2-ethyl-N4-isopropyl-1,3,5-triazine-2,4-diamine) is frequently detected at high concentrations in ground water. Bentazone [3-isopropyl-1H-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide] plus alachlor (2-chloro-2',6'-diethyl-N-methoxymethylacetanilide) is a potential herbicide combination used as a substitute for atrazine. Thus, the objective of this study was to assess the environmental risk of this blend. Drainage water contamination by bentazone and alachlor was assessed in silty clay (Vertic Eutrochrept) and silt loam (Aquic Hapludalf) soils under the same management and climatic conditions. Drainage volumes and concentrations of alachlor and bent…

Laboratory leaching studies of oryzalin and diuron through three undisturbed vineyard soil columns

Article de revue (Article scientifique dans une revue à comité de lecture); International audience; The leaching of diuron and oryzalin through undisturbed soil columns was studied in the laboratory using three vineyard soils from Vosne-Romanée (Burgundy): a rendosol, a calcosol and a vegetated calcosol. After 845 mm of simulated rainfall in 15 days, soil leachates contained higher amounts of diuron (3.2%, 11.8% and 18.8% of applied diuron, respectively) than oryzalin (0.2%, 4.9%, 3.7%, respectively). A greater proportion of soil extractable residues was obtained for diuron (42.5%, 26.8% and 32.2%, respectively) than for oryzalin (14.7%, 12% and 15.5%, respectively). The greater mobility of…

Influence of organic amendments on diuron leaching through an acidic and a calcareous vineyard soil using undisturbed lysimeters.

9 pages; International audience; The influence of different organic amendments on diuron leaching was studied through undisturbed vineyard soil columns. Two composts (A and D), the second at two stages of maturity, and two soils (VR and Bj) were sampled. After 1 year, the amount of residues (diuron þ metabolites) in the leachates of the VR soil (0.19e0.71%) was lower than in the Bj soil (4.27e8.23%), which could be explained by stronger diuron adsorption on VR. An increase in the amount of diuron leached through the amended soil columns, compared to the blank, was observed for the Bj soil only. This result may be explained by the formation of mobile complexes between diuron and water-extrac…

Leaching of oryzalin and diuron through undisturbed vineyard soil columns under outdoor conditions

12 pages; International audience; Field studies monitoring herbicide pollution in the vineyards of Burgundy (France) have revealed that drinking water reservoirs are contaminated with several pre-emergence herbicides. An assessment of the leaching of two such herbicides, diuron and oryzalin, was therefore performed using lysimeters, under outdoor conditions, from May 2001 to May 2002. Four vineyard soils from Vosne-Roman?(Burgundy) were chosen along a topolithosequence: a rendosol and three calcosols. After 673 mm of rainfall, greater amounts of diuron than oryzalin were measured in percolates: respectively 0.10-0.84% and 0.02-0.43% of applied herbicide, depending on soils. Measurements for…

Compost Effect on Diuron Retention and Transport in Structured Vineyard Soils

12 pages; International audience; Diuron is frequently detected in surface- and groundwater under the vineyards, where organic amendments are often used, in Burgundy of France. Undisturbed column experiments were conducted to study the influence of three composted organic amendments on diuron leaching through columns of two vineyard soils from Vosne-Romanée (VR, calcareous Cambisol) and Beaujolais (Bj, sandy Leptosol), France. Bromide (used as non-reactive tracer) and diuron breakthrough curves (BTCs) were analyzed using convectivedispersive equation (CDE), two-region (mobile-immobile, MIM) and two-site models. No influence of the composts was observed on the bromide recovery rates. The CDE…

Water-stable aggregates and organic matter pools in a calcareous vineyard soil under four soil-surface management systems

Facilitated transport of diuron and glyphosate in high copper vineyard soils.

6 pages; International audience; The fate of organic herbicides applied to agricultural fields may be affected by other soil amendments, such as copper applied as a fungicide. The effect of copper on the leaching of diuron and glyphosate through a granitic and a calcareous soil was studied in the laboratory using sieved-soil columns. Each soil was enriched with copper sulfate to obtain soil copper concentrations of 125, 250, 500, and 1000 mg kg(-1). Glyphosate leaching was influenced by soil pH and copper concentration, whereas diuron leaching was not. In the calcareous soil, glyphosate leaching decreased as copper levels increased from 17 mg kg(-1) (background) to 500 mg kg(-1). In the gra…

Microbial acidification and pH effects on trace element release from sewage sludge.

Leaching of sludge-borne trace elements has been observed in experimental and field studies. The role of microbial processes in the mobilization of trace elements from wastewater sludge is poorly defined. Our objectives were to determine trace element mobilization from sludge subjected to treatments representing microbial acidification, direct chemical acidification and no acidification, and to determine the readsorption potential of mobilized elements using calcareous sand. Triplicate columns (10-cm diameter) for incubation and leaching of sludge had a top layer of digested dewatered sludge (either untreated, acidified with H2SO4, or limed with CaCO3; all mixed with glass beads to prevent …

Evaluating equilibrium and non-equilibrium transport of bromide and isoproturon in disturbed and undisturbed soil columns.

19 pages; International audience; In this study, displacement experiments of isoproturon were conducted in disturbed and undisturbed columns of a silty clay loam soil under similar rainfall intensities. Solute transport occurred under saturated conditions in the undisturbed soil and under unsaturated conditions in the sieved soil because of a greater bulk density of the compacted undisturbed soil compared to the sieved soil. The objective of this work was to determine transport characteristics of isoproturon relative to bromide tracer. Triplicate column experiments were performed with sieved (structure partially destroyed to simulate conventional tillage) and undisturbed (structure preserve…

Water-stable aggregates and organic matter pools in a calcareous vineyard soil under four soil-surface management systems

. Vineyards in Champagne, France are generally situated on slopes where the soils are subject to erosion. Therefore it is important to find a soil-surface management practice that protects the soil against water erosion. We assessed the potential of mulches or grass covers to stabilize soil aggregates in a calcareous sandy loam from a vineyard in Champagne after 9 years under different management systems. Four different treatments were studied: (i) a bluegrass (Poa pratensis) surface cover between the vine rows (GC) with bare soil under the vines (R); two organic mulches of (ii) coniferous (CB) or (iii) poplar (PB) bark that covered the entire soil surface, and (iv) bare soil between the ro…

Diuron mobility through vineyard soils contaminated with copper

International audience; The herbicide diuron is frequently applied to vineyard soils in Burgundy, along with repeated treatments with Bordeaux mixture (a blend of copper sulfate and calcium hydroxide) that result in elevated copper concentrations. Cu could in principle affect the fate and transport of diuron or its metabolites in the soil either directly by complexation or indirectly by altering the populations or activity of microbes involved in their degradation. To assess the effect of high Cu concentrations on diuron transport, an experiment was designed with ten undisturbed columns of calcareous and acidic soils contaminated with 17–509 mg kg−1 total Cu (field-applied). Grass was plant…

Isolation and characterisation of Nocardioides sp. SP12, an atrazine-degrading bacterial strain possessing the gene trzN from bulk- and maize rhizosphere soil

International audience; We report the characterisation of Nocardioides sp. SP12, an atrazine-degrading bacteria isolated from atrazine-treated bulk- and maize rhizosphere soil. Based on 16S rDNA alignment, strain SP12 showed close phylogenic relationships with Nocardioides sp. C157 and Nocardioides simplex. Internal transcribed spacer (ITS) sequences of strain SP12 were longer than those of other Nocardioides sp. and present Ala- and Ile-tRNA unlike Actinomycetales. Nocardioides sp. SP12 presents a novel atrazine catabolic pathway combining trzN with atzB and atzC. Atrazine biodegradation ends in a metabolite that co-eluted in HPLC with cyanuric acid. This metabolite shows an absorption spe…