Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy
AbstractThe current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2) highlights the need for coordinated research to combat COVID-19. A particularly important aspect is the development of medication. In addition to viral proteins, structured RNA elements represent a potent alternative as drug targets. The search for drugs that target RNA requires their high-resolution structural characterization. Using nuclear magnetic resonance (NMR) spectroscopy, a worldwide consortium of NMR researchers aims to characterize potential RNA drug targets of SCoV2. Here, we report the characterization of 15 conserved RNA elements located at the 5′ end, the ribosomal frameshift segment and t…
Structure-Based Design of an RNA-Bindingp-Terphenylene Scaffold that Inhibits HIV-1 Rev Protein Function
Inside Back Cover: Structure-Based Design of an RNA-Bindingp-Terphenylene Scaffold that Inhibits HIV-1 Rev Protein Function (Angew. Chem. Int. Ed. 50/2013)
Cognición social y habilidades de comunicación en adultos jóvenes con síndrome de Asperger
Abstract: The aim of this study is to compare patients with Asperger syndrome (AS) and control participants on social cognition tasks and communication skills. Twenty young adults were assessed in three social cognition domains; recognition of basic emotions, recognition of complex emotions and Theory of Mind (ToM). In addition participants completed a self-report questionnaire as a measure of social communication skills. The results indicate that adults with AS perform below neurotypical control participants in emotion processing, ToM and communication skills. There were no significant correlations between the variables of social cognition and communication skills in the group of AS. Taken…
Imbalance of immunological synapse-kinapse states reflects tumor escape to immunity in glioblastoma
Since the proper activation of T cells requires the physical interaction with target cells through the formation of immunological synapses (IS), an alteration at this level could be a reason why tumors escape the immune response. As part of their life cycle, it is thought that T cells alternate between a static phase, the IS, and a dynamic phase, the immunological kinapse (IK), depending on high or low antigen sensing. Our investigation performed in tissue samples of human glioma shows that T cells are able to establish synapsing interactions not only with glioma tumorigenic cells, but also with stromal myeloid cells. Particularly, the IS displaying a T cell receptor-rich (TCR-rich) central…
[Transphenoidal endoscopic approaches for pituitary adenomas: a critical review of our experience].
Abstract Background The surgical approach to the pituitary fossae has evolved from transcranial to sublabial and transseptal microscopic ones, up to the current transsphenoidal endoscopic approach. Objectives To present our experience in the transnasal transsphenoidal approach for pituitary adenomas and the modifications introduced to improve tumoral resection and to lower iatrogenia. Material and methods Over nine years, we operated on 37 patients with pituitary adenomas using the transsphenoidal endoscopic approach. We utilised optical lens of 0° (approach) and 30° (adenoma resection), optic navigator, surgical instruments for nasal endoscopic and pituitary surgery. During the neurosurgic…
Continuous intra-amygdalar infusion of GABA in the amygdala kindling model of epilepsy in rat.
Objective: To explore the effect of continuous intra-amygdalar infusion of GABA in the amygdala kindling model of epilepsy in rat. Methods: An electrode and cannula complex was implanted in adult male Wistar rats, the electrode being targeted to the left basolateral amygdala. The animals were subjected to a standard kindling procedure. Osmotic minipumps filled with either GABA or mannitol were connected to cannulas and allowed to infuse during 7 days. Kindling experiments measuring after-discharge and seizure thresholds, seizure severity and duration, and behavioral toxicity were performed before, during and after the drug infusion period. Results: Both after-discharge and seizure threshold…
Abordajes transesfenoidales endoscópicos de los adenomas hipofisarios: una revisión crítica de nuestra experiencia
Resumen Introduccion El abordaje quirurgico de la region hipofisaria ha evolucionado desde las vias transcraneales, a las sublabiales y transseptales microscopicas hasta los abordajes endoscopicos actuales. Objetivos El objetivo de este trabajo es exponer y reflexionar sobre nuestra experiencia en el abordaje transnasal transesfenoidal endoscopico de los tumores hipofisarios, con las modificaciones que hemos introducido para mejorar la exeresis del tumor y disminuir la iatrogenia. Material y metodos A lo largo de 9 anos hemos intervenido a 37 pacientes afectos de adenomas hipofisarios mediante abordaje transesfenoidal endoscopico. Utilizamos lentes de 0 y 30 grados, navegador optico, instru…
Role of Deprotonation and Cu Adatom Migration in Determining the Reaction Pathways of Oxalic Acid Adsorption on Cu(111)
Scanning tunneling microscopy (STM), X-ray photoelectron spectroscopy (XPS), and first principles theoretical calculations have been used to gain insight into the fundamental processes involved in the adsorption and self-assembly of oxalic acid on Cu(111). The experimental data demonstrate that several reaction pathways are involved in the chemisorption of oxalic acid on Cu(111), one of which leads to deprotonation of the acid into oxalate molecules that form ordered structures on the surface. Theoretical calculations indicate that the adsorption of oxalate molecules is not stable on the surface unless copper adatoms are taken into consideration. Coordination with copper adatoms prevents ox…
The Study of Carbamoyl Phosphate Synthetase 1 Deficiency Sheds Light on the Mechanism for Switching On/Off the Urea Cycle
12 páginas, 4 figuras, 2 tablas.