Development of a new multiplex assay for STR typing of telogen hair roots

Abstract We have developed a new strategy in which 10 STR systems plus amelogenin were simultaneously amplified from telogen hair roots with maximal fragment sizes smaller than 270 base pairs. The multiplex includes six STR loci from the European standard set of loci (ESS) for DNA databases (D3S1358, D8S1179, D21S11, THO1, FGA and VWA) as well as four additional STR systems selected for their robustness and short amplicon sizes (D2S1338, D12S391, TPOX and D5S818). Due to the biotinylation of the reverse primers from five STRs systems, these PCR products can be separated from the other six amplicons after PCR amplification. The two sub-multiplexes were then analyzed in two different runs on …

Bgl II restriction fragment length polymorphism of human complement C4A gene coincides with BF*F allele of factor B.