6533b7d1fe1ef96bd125d60e
RESEARCH PRODUCT
Development of a new multiplex assay for STR typing of telogen hair roots
P.m. SchneiderP.m. SchneiderK. Bendersubject
GeneticsBase pairSTR multiplex systemGeneral MedicineBiologyAmpliconMolecular biologylaw.inventionCapillary electrophoresisSTR analysislawMultiplexAmelogeninPolymerase chain reactiondescription
Abstract We have developed a new strategy in which 10 STR systems plus amelogenin were simultaneously amplified from telogen hair roots with maximal fragment sizes smaller than 270 base pairs. The multiplex includes six STR loci from the European standard set of loci (ESS) for DNA databases (D3S1358, D8S1179, D21S11, THO1, FGA and VWA) as well as four additional STR systems selected for their robustness and short amplicon sizes (D2S1338, D12S391, TPOX and D5S818). Due to the biotinylation of the reverse primers from five STRs systems, these PCR products can be separated from the other six amplicons after PCR amplification. The two sub-multiplexes were then analyzed in two different runs on a capillary gel electrophoresis instrument.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-04-01 | International Congress Series |