Relationship between canopy architecture and fruit quality on 'Rich May' peach grafted onto 'Penta' and 'GF677' rootstocks

Observations on crop efficiency and fruit quality of peach trees of ‘Rich May’ grafted onto ‘GF677’ (peach x almond) and ‘Penta’ (Prunus domestica L.) rootstocks are reported. Results were related to canopy architecture parameters, particularly to scaffold branches, shoot and leaf blade inclination angles, to the pattern of light distribution, and to the area of sunflecks projected at midday on the ground. Trees grafted onto ‘GF677’ were more vigorous, in terms of trunk cross-sectional area, summer pruning weight and leaf area. Within canopy light distribution profiles were in agreement with foliage density, with a higher light interception observed in trees grafted on ‘GF677’. Within canop…

Growth and yields of 'Arbequina' high-density planting systems in three different olive growing areas in Italy

The sustainability of the traditional olive-growing sector in EU countries characterised by high production costs and a low selling price for the oil was mainly determined by EU subsidies available for the sector. With the opening of the free trade area by the Barcellona Declaration of 1995 and a cut in EU subsidies in 2014, crucial changes in the sector are now needed. In order to increase the competitiveness of EU olive production, attention should be given to new high-yielding and mechanized cultivation systems. In the 1990s, Spain introduced new high-density planting systems (1,200-2,000 trees/ha) using three low-vigour and early-fruiting cultivars ( Arbequina , Arbosana and Koroneiki )…

Identification of (In)Compatible S-genotypes and Molecular Characterisation of Italian Sweet Cherry Cultivars.

Italy is the first sweet cherry producer in Europe. Its rich germplasm of local varieties has not been fully characterised or exploited in breeding programmes. Sweet cherry is a self-incompatible species; this trait is controlled by a gametophytically expressed multi-allelic (S) locus. The knowledge of sweet cherry S-alleles and cross-incompatibility groups is important for growers and breeders for choosing appropriate pollinators in the orchard and planning crosses. In this work, we analysed 94 sweet cherry cultivars native of large part of the Italian regions where cherry is grown. The microsatellite primer pair set and the reference cultivars agreed for cherry by the Prunus Working Group…

Evaluation of carotenoids and furosine content in air dried carrots and parsnips pre-treated with pulsed electric field (PEF)

Carrot and parsnip slices were subjected to hot drying at 50, 60 and 70 °C with or without pulsed electric field (PEF) pre-treatment at 0.9 kV/cm and 1000 and 10,000 pulses. The effect of drying on processed samples was assessed by analysis of carotenoids, the furosine value, total phenols and the Whiteness Index (WI). PEF pre-treatment is effective in reducing drying times, but the degree of cellular breakdown makes the compounds more susceptible to chemical and enzymatic reactions. In our condition PEF pre-treatment increased the carotenoid degradation and promoted the Maillard reaction above all at the highest temperature of 70 °C. The assessment of carotenoid stability, together with th…

Effect of Pulsed Electric Field Pretreatment on Drying Kinetics, Color, and Texture of Parsnip and Carrot.

The aim of the study was to evaluate the effect of pulsed electric field (PEF) pretreatment on drying kinetics and on color and textural changes in sliced parsnip and carrot. Cell disintegration index measured in parsnips after PEF pretreatment significantly differed from the ones measured for carrots and, consequently, the reduction of the time needed to dry both samples to a final moisture content up to 5% (wet basis). The drying time of the PEF pretreated parsnip was reduced by up to 28% at 70 °C and by up to 21% at 60 °C in carrot, in comparison to untreated samples. The PEF pretreatment influenced the effective diffusivity of water into the samples going from 50 °C to 70 °C: in carrots…

PHENOLOGICAL AND MORPHOLOGICAL STUDIES OF PISTACIA TEREBINTHUS L. GENOTYPES NATIVE OF BULGARIA WITH DIFFERENT ASSET OF TREE SEXUALITY

The genus Pistacia is a dioecious species staminate and pistillate inflorescences born on different trees and are wind pollinated. In pistachio orchards, female and male flowering periods are often not synchronized. In fact, male trees spread their pollen before the flowers of female trees become receptive. Pollination is commonly devoted to spontaneous males of P. terebinthus. In this research authors refer to the results obtained observing the phenology of the blooming period of monoicous genotypes of P. terebinthus discovered in the Rhodope Mountains (Bulgaria). In some selected trees phenological stages have been observed in situ. The blooming date is influenced by local environmental c…

Water status and gas exchange of pistachio trees under different irrigation levels

The aim of this study was to investigate the physiological responses of pistachio trees to varying water supply in the attempt to identify reliable parameters to manage irrigation. Three different irrigation regimes were applied to 25-year-old pistachio trees of cultivar 'Bianca' on P. terebinthus rootstock. Midday stem water potential (ΨSWP), leaf relative water content (RWC), light-saturated net CO2assimilation (An), stomatal conductance (gs), and ambient conditions were monitored throughout the season, and light response curves of net photosynthesis were constructed. To reflect tree water status, trees were separated into three classes of ΨSWP, no deficit (ΨSWP> -1.5 MPa), mild defici…

Seasonal dynamics of photosynthesis and total carbon gain in bearing and nonbearing pistachio (Pistacia vera L.) shoots

Seasonal changes in leaf gas exchange, assimilation response to light and leaf area were monitored in bearing and nonbearing pistachio shoots. Shoot bearing status did not directly affect leaf photosynthetic rate. However, photosynthetic light-response curves strongly varied during the season demonstrating the dominant effect of the tree’s seasonal phenology on assimilation. Early in the season low photosynthetic rates were associated with high rates of dark respiration indicating limited photosynthesis in the young leaves. As leaves matured, dark respiration decreased and assimilation reached maximum values. Photosynthetic efficiency was strongly reduced late in the season due to leaf ag…

Impact of freezing rate on electrical conductivity of produce

The aim of this work was to compare the effects of freezing rate on electrical conductivity of potatoes, carrots and apples. Electrical conductivity tests were conducted on a custom ohmic cell while samples texture was measured by means of a universal testing machine. The raw un-pretreated samples were used as control. This study showed that freezing pre-treatments lead to differences in electrical conductivity of considered samples, producing structural damage, the latter being relatively more severe when the tested products undergo ohmic treatment.

Sustainability of pistachio production (Pistacia vera L.) under supplemental irrigation in a Mediterranean climate

Abstract The objective of this study was to examine the effects of supplemental micro-irrigation, in a commercial rainfed orchard, on tree water status, gas exchange and productivity of Pistacia vera L. (cv Bianca) grafted on P. terebinthus. Irrigating with 10–15% of estimated full evaporative demand significantly increased average annual productivity by increasing yield in the on-crop year of this alternate bearing species. Irrigation increased yield by 30%, resulting in 1600 kg of in-shell product per ha. Irrigation delayed leaf senescence while flower bud abscission was not reduced until the third year of experiment. Irrigation did not improve gas exchange during Stage I (pericarp growth…

Genetic diversity and clonal variation within the main Sicilian olive cultivars based on morphological traits and microsatellite markers

The richness of Olea europaea (L.) genetic resources in Sicily is well documented. In the last 30 years, mostof the local cultivars, landraces and ecotypes have been gathered together in a large ex-situ collection,containing more than 300 genotypes. In this study, 45 putative clones of the main Sicilian olive cultivarswere characterized morphologically using microsatellite markers to unambiguously identify possiblesuperior genotypes. The microsatellites employed were polymorphic (observed heterozygosity = 0.71;polymorphic information content = 0.59), discriminated 52% of the genotypes and enabled the detectionof intra-cultivar polymorphism, derived from both somatic mutations, indicating th…

Heat requirements for loquat fruit development may be assessed with a Beta model approach

In Mediterranean areas, loquat trees (Eriobotrya japonica Lindl.) typically bloom in fall and fruits develop during the winter period, reaching their ripening in spring. So far, little interest has been driven towards models capable of estimating heat requirements for the accomplishment of fruit development in the winter period. In this work, we verified whether in Mediterranean areas the heat accumulation (GDH) needed for loquat fruit growth, may be precisely described using a Beta model, a flexible mathematical function which, in the past, has been successfully used in peach. BBCH phenological stages of ‘Algerie’ trees were recorded in two experimental sites: Palermo, Italy (38°04’N, 13°2…

Seasonal variations of antimicrobial activity and chemical composition of essential oils extracted from three Citrus limon L. Burm. cultivars

In order to investigate the seasonal variations of antimicrobial properties and chemical composition of essential oils (EOs), three different cultivars of Citrus limon L. Burm. spp. (Femminello Santa Teresa, Monachello and Femminello Continella) were collected at 6-week intervals, from December 2012 to April 2013, for a total of four harvests. The EOs were extracted from lemon peel by hydro-distillation. The antimicrobial activity, tested by paper disc diffusion method, was evaluated against common food-related pathogenic bacteria (Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica and Enterobacter spp.). EOs were more effective against Gram-positive than Gram-negative bacte…

Preliminary identification of self-incompatibility genotypes of Sicilian almond landraces

Sicily is the main almond producer in Italy. The almond tree is one of the main protagonists of the Sicilian agrarian landscape, especially in the winter, when the bare branches are covered by white or pinkish flowers. Its presence is particularly significant in the province of Agrigento, in Syracuse and in Ragusa areas, where the almond nut is used for fresh consumption and for the preparation of typical sweets. The extremely rich Sicilian almond germplasm can be useful for genetic improvement, since many local landraces possess aromatic characteristics of the nuts of high value. Self-incompatibility in this species is gametophytic and controlled by a multi-allelic S locus, coding for the …

THERMAL TIME REQUIREMENT AND HARVEST TIME FORECAST FOR PEACH CULTIVARS WITH DIFFERENT FRUIT DEVELOPMENT PERIODS

Non-linear models using growing degree hours (GDH), based on the choice of base, critical and optimum temperatures, have been successfully applied to calculate thermal time required for spring bud burst in deciduous fruit trees. The flexibility of the model can fit the wide range of temperatures that occur during the peach fruit development period (FDP), which takes place from early spring to late summer. In this experiment, fruit growth was studied in relation to thermal time accumulated from bloom to fruit harvest for peach and nectarine cultivars whose fruit development period range from 70 to 150 days. Thermal time was calculated in terms of degree days (DD) (base temperature 7 °C, and …

Growth, yield and fruit quality of 'Tropic Snow' peach on size-controlling rootstocks under dry Mediterranean climates

Abstract A six-year trial was carried out to evaluate the vegetative and productive performance of low-chill, early-ripening ‘Tropic Snow’ peach grown on five peach × almond rootstocks, different for their vigor. The study was conducted in south-western Sicily using 45 V-trained peach trees planted in 2005 and grafted on GF677, the most common rootstock in Sicily, and four low-vigor rootstocks selected at the Department of Agricultural, Food and Environmental Sciences, University of Pisa, namely IS5/8 (Polluce), IS5/19 (Castore), IS5/23, and IS5/29. Trunk circumference, winter and summer pruning weight, total number and weight of fruits per tree, and major fruit quality traits were recorded…

Genetic relationships, structure and parentage simulation among the olive tree (Olea europaea L. subsp. europaea) cultivated in Southern Italy revealed by SSR markers

In this work, we assess both the morphological and genetic diversity of 68 important olive cultivars from three Southern Italian regions: Calabria, Campania and Sicily. Twenty-five phenotypic traits were evaluated and 12 simple sequence repeat (SSR) markers were analysed. All SSR primers were polymorphic and reliable. The total number of alleles per locus varied from 5 to 19 with an average number of 13.1 and a mean polymorphic information content (PIC) of 0.81. These results suggested high genetic diversity within these three olive germplasm collections. Morphological traits also showed significant variability amongst cultivars. Two cases of identity were found and ten statistically signif…

Toward the definition of a carbon budget model: seasonal variation and temperature effect on respiration rate of vegetative and reproductive organs of pistachio trees (Pistacia vera)

Summary This study, as a preliminary step toward the definition of a carbon budget model for pistachio trees (Pistacia vera L.), aimed at estimating and evaluating the dynamics of respiration of vegetative and reproductive organs of pistachio tree. Trials were performed in 2005 in a commercial orchard located in Sicily (370 m a.s.l.) on five bearing 20-year-old pistachio trees of cv. Bianca grafted onto Pistachio terebinthus L. Growth analyses and respiration measurements were done on vegetative (leaf) and reproductive (infructescence) organs during the entire growing season (April–September) at biweekly intervals. Results suggested that the respiration rates of pistachio reproductive and v…

RNA-Seq analysis to investigate alternate bearing mechanism in Pistacia vera L

Pistachio (Pistacia vera L.) production suffers a high level of alternate bearing. The mechanism underlying this negative phenomenon is different from other species, such as apple and olive. Pistachio produces a high number of inflorescence buds every year that in heavy cropping trees (“ON”) mostly fall during the kernel development phase, which occurs in July-August. Primary metabolites (i.e., carbohydrates) play a key role in the signaling related to inflorescence bud abscission. In this work, RNA-Seq was used as a tool to investigate transcriptome of inflorescence buds and fruits, sampled from branches with low (“OFF”) and high (“ON”) crop load. Reference based RNA-Seq analysis using Ara…

Predicting olive flowering phenology with phenoclimatic models

In plants, day length and temperature are the major climatic factors that affect the transition from a phenological phase to the next one. Non-linear models, such as growing degree hours (GDH), have been successfully used to calculate thermal time required for spring bud burst in deciduous fruit trees. In this experiment, temperature records and blooming dates of olive trees in different years and for 10 different sites in the Italian territory were recorded. Olive booming time was correlated to the amount of (GDH) accumulated from the date of bud rest onset, calculated as the day when the maximum negative chilling units accumulation was reached (UTAH Model), to full bloom. The GDH model wa…

Effect of soil permanent grass cover on growth, yield and water status of rainfed olive trees in Sicily

The study was carried out in an olive grove located along the hills of northern Sicily where soil had been managed for decades by chemical weeding. Starting in 2008, one portion of the grove was left non-weeded, and the permanent grass cover was managed by mowing 2-3 times per year in winter and spring. In 2011, 2012 and 2013, yield, average drupe weight, percentage of drupe black color (veraison), trunk circumference, shoot elongation, and leaf relative water content (RWC) were determined on 34 adult trees of the cultivar 'Biancolilla' with uniform size and age. In 2013, soil organic matter content and leaf nutrient concentrations were also determined in the two portions of the grove. As e…

Horticultural management of Italian Pistachio orchard systems: current limitations and future prospective

Pistachio production and consumption have strongly increased in the last years due to its capability to grow under limiting conditions, the profitability of this nut in the market and its well claimed healthy properties. The Sicilian pistachio industry is diminutive in comparison to big producing countries such as California and Iran. Its economical profitability is limited by a low productivity per surface area, strong alternate bearing, and long unproductive period, mainly associated with the lack of implementation of innovative management practices. This “traditional” agricultural system is surviving in a highly competitive international market thanks to the unique organoleptic profile o…

Evaluation of Small Vase and Y-trellis Orchard Systems for Peach and Nectarine Production in Mediterranean Regions

Two peach planting systems, Small Vase (SV) and Y-trellis (Y), were evaluated and compared in the Mediterranean settings of Southern Italy. The two orchards were located next to each other on relatively uniform soil and terrain, and the observations included two peach (‘Rich May’ and ‘Summer Rich’) and two nectarine (‘Big Bang’ and ‘Nectaross’) cultivars. In the SV system, trees were spaced at 4.5 A— 2.5 m (888 trees/ha), whereas in the Y system, trees were spaced at 5.5 A— 2 m (909 trees/ha) and no canopy gap was left between rows. Yield per tree; fruit size grade; unit price of sold peaches for each size grade; materials, labor and associated costs for cultural management;…

A sustainable phenolic compound extraction system from olive oil mill wastewater

Abstract The reduction of environmental impact and the achievement of economic advantages based on by-product recovery is the aim of applying methodologies for phenolic compounds extraction from Olive Oil Mill Wastewater. Volume reduction of Olive Oil Mill Wastewater could generate important advantages in terms of waste disposal cost abatement and, in addition, reuse in the production of foods with health properties could lead to economic benefits. Despite the fact that literature shows that several techniques have been developed, few economic approaches have been presented. This paper assesses the economic feasibility of a system based on membrane filtration and reverse osmosis processes f…

Horticultural performance of 23 Sicilian olive genotypes in hedgerow systems: Vegetative growth, productive potential and oil quality

Abstract The super high density (SHD) model is a new olive growing system characterized by earlier and higher yields, fully mechanized harvesting and reduced orchard management costs. Until recently all commercial SHD orchards were planted primarily with three varieties: ‘Arbequina’, ‘Arbosana’ and ‘Koroneiki’. To increase variety diversity, broaden available olive oil chemical and organoleptic profiles, and olive oils for marketing, minor local varieties should be evaluated for adaptability to the SHD system. This study compares multiple Sicilian native genotypes to the three current cultivars ‘Arbequina’, ‘Arbosana’ and ‘Koroneiki’. The cumulative fruit and oil production, trunk-cross sec…

New selections of Prunus persica for low chill Mediterranean climate areas

In the late 1990s, CREA and the University of Palermo initiated a peach-breeding program in order to select new genotypes suitable for the fruit growing areas of southern Italy. These regions are generally characterized by short, mild winters and long, hot, dry summers. International cultivars grown in continental environments often have problems related to climatic limitations, such as the failure to satisfy the winter chill requirements. About 40 different peach and nectarine cultivars with different fruit characteristics (size, skin over colour, flesh firmness, sugar content, titratable acidity, absence of split-pit) were used as parents in a breeding program by CREA. The best selections…

Toward the valorization of olive (Olea europaea var. europaea L.) biodiversity: horticultural performance of seven Sicilian cultivars in a hedgerow planting system

Abstract An intense survey of the Sicilian’s olive growing areas for autochthonous germplasm, mainly represented by centennials olive trees (Olea europaea var. europaea L.) apparently older then III centuries, started at the beginning of the 1980s and resulted in the selection of more than 150 cultivars and accessions. This germplasm was propagated in a nursery, by grafting onto seedlings of Olea europaea L., and planted in an experimental orchard, in an olive district located in the South-west of the Island, where they were evaluated for over 30 years and selected for their early bearing, high and constant productivity, as well as high oil content of the fruits and excellent chemical (olei…

Ecophysiological Characterization of the Canopy of Peach (P. persica L. Batsch) in Two Planting Systems

Responses of Young Peach Trees to Root Confinement

Rooted cuttings of Nemaguard peach [Prunus persica (L.) Batsch.] were grown in 0.18-, 0.36-, 0.90-, and 2.40-liter containers for 16 weeks to study the influence of root confinement on growth, gas exchange, water uptake, and leaf carbohydrate and nutrient content. An automatic nutrient-solution dispensing system was used to ensure uniform fertility among treatments and to prevent drought stress. Leaf area and stem length were reduced by root confinement 6 to 7 weeks after transplanting, and differences among treatments increased throughout the experiment. Final tree dry weights were reduced by 51% over a 13-fold reduction in rooting volume, but dry weight partitioning was largely unaffected…

High-resolution UAV imagery for field olive (Olea europaea L.) phenotyping

Remote sensing techniques based on images acquired from unmanned aerial vehicles (UAVs) could represent an effective tool to speed up the data acquisition process in phenotyping trials and, consequently, to reduce the time and cost of the field work. In this study, we assessed the ability of a UAV equipped with RGB-NIR cameras in highlighting differences in geometrical and spectral canopy characteristics between eight olive cultivars planted at different planting distances in a hedgerow olive orchard. The relationships between measured and estimated canopy height, projected canopy area and canopy volume were linear regardless of the different cultivars and planting distances (RMSE of 0.12 m…

Mathematical Model of Solid Food Pasteurization by Ohmic Heating: Influence of Process Parameters

Pasteurization of a solid food undergoing ohmic heating has been analysed by means of a mathematical model, involving the simultaneous solution of Laplace’s equation, which describes the distribution of electrical potential within a food, the heat transfer equation, using a source term involving the displacement of electrical potential, the kinetics of inactivation of microorganisms likely to be contaminating the product. In the model, thermophysical and electrical properties as function of temperature are used. Previous works have shown the occurrence of heat loss from food products to the external environment during ohmic heating. The current model predicts that, when temperature gradient…

Planting density, above-ground dry-matter partitioning and fruit quality in greenhouse-grown 'Flordaprince' peach (Prunus persica L. Batsch) trees trained to 'free-standing Tatura'

SummaryThe effect of planting density on yield, fruit quality and above-ground dry-matter partitioning into vegetative and reproductive organs of six year old peach trees was investigated in Sicily on the very early-ripening cv Flordaprince in greenhouse. Increasing the number of trees per hectare from 1500 to 5000 significantly reduced total above-ground dry matter, trunk cross-sectional area and total leaf area. Differences in growth did not result in substantial modifications in the source-sink relationships between the vegetative and the reproductive organs of the above-ground part of the tree, as shown by the lack of statistical differences in dry-matter partitioning into fruit, leaves…

Effects of different irrigation regimes on a super-high-density olive grove cv. “Arbequina”: vegetative growth, productivity and polyphenol content of the oil

The effects of multiple irrigation regimes on the relationships among tree water status, vegetative growth and productivity within a super-high-density (SHD) “Arbequina” olive grove (1950 tree/ha) were studied for three seasons (2008–2010). Five different irrigation levels calculated as percentage of crop irrigation requirement using FAO procedures (Allen et al. in Crop evapotranspiration. Guidelines for computing crop water requirements. Irrigation and drainage paper 56. FAO, Rome, 1998) were imposed during the growing season. Periodically during the growing season, daytime stem water potential (Ψ STEM), inflorescences per branch, fruits per inflorescence and shoot absolute growth rate wer…

Transcriptome Analysis of Pistacia vera Inflorescence Buds in Bearing and Non-Bearing Shoots Reveals the Molecular Mechanism Causing Premature Flower Bud Abscission

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Transcriptomic responses to biotic stresses in Malus x domestica: a meta-analysis study.

AbstractRNA-Seq analysis is a strong tool to gain insight into the molecular responses to biotic stresses in plants. The objective of this work is to identify specific and common molecular responses between different transcriptomic data related to fungi, virus and bacteria attacks in Malus x domestica. We analyzed seven transcriptomic datasets in Malus x domestica divided in responses to fungal pathogens, virus (Apple Stem Grooving Virus) and bacteria (Erwinia amylovora). Data were dissected using an integrated approach of pathway- and gene- set enrichment analysis, Mapman visualization tool, gene ontology analysis and inferred protein-protein interaction network. Our meta-analysis revealed…

Genetic similarity among Tunisian cultivated olive estimated through SSR markers

Olive (Olea europaea L. subsp. europaea var. europaea) is one of the oldest fruit tree in the Mediterranean basin, and is cultivated for oil and canned fruit. Part of this interest is driven by the economic importance of olive oil which is increasing throughout the world due to its beneficial effect to human health. In Tunisia, olive has great socio-economic importance, with more than 60 millions olive trees cultivated for olive oil production including a wide range of cultivars which are widely extended from the north to the south regions of the country for its high economic value. Here, we applied microsatellites (SSRs) molecular markers to assess the genetic variability of the most impor…

Musculoskeletal Pain in Gymnasts: A Retrospective Analysis on a Cohort of Professional Athletes

Gymnastics athletes are exposed to a high risk of injury, but also of developing musculoskeletal pain. These data are still little investigated in the available scientific literature. An online survey was distributed to 79 professional athletes who practiced artistic and rhythmic gymnastics. The survey collected demographic and anthropometric data, information about the sport practice, the training sessions, the prevalence of musculoskeletal pain gymnastics-related, and lifestyle habits. Musculoskeletal pain had a high prevalence, involving 65 of 79 athletes (82.3%). A significant correlation was found between musculoskeletal pain and the duration of sports practice, both for general pain (…

Use of phenoclimatic models to estimate the chill and heat requirements of four sweet cherry cultivars in Italy

During their dormant period, fruit trees require a certain amount of relatively low temperatures to complete bud differentiation processes and regularly resume growth the following spring. Unsatisfied chilling needs can result in delayed foliation, severe yield and fruit quality losses. There is a need for a method to estimate accurately the chilling requirement of a cultivar. This is especially important in those areas where the potential of chill accumulation in winter is low and for those crops, such as sweet cherry, which generally have a high chilling requirement. Historical temperature records and blooming dates of four sweet cherry cultivars widely cultivated in Italy - 'Burlat', 'Sw…

Genetic diversity of fig (Ficus caricaL.) genotypes grown in Southern Italy revealed by the use of SSR markers

The genetic variability among 181 fig (Ficus carica L.) accessions found in small farms located in Campania, Basilicata, Apulia, Calabria and Sicily was investigated analysing the polymorphism of 18 simple sequence repeat (SSR) markers. The SSR analysis revealed a large genetic diversity among accessions. A total of 117 alleles were detected with a mean of 6.5 locus-1. The average expected (He) and observed heterozygosity (Ho) were 0.56 and 0.66, respectively. The mean polymorphic information content (PIC) was 0.51, suggesting a significant molecular diversity among the fig accessions taken into consideration. The UPGMA cluster analysis discriminated 174 genotypes and allowed to find 8 grou…

Algerian Olive Germplasm and Its Relationships with the Central-Western Mediterranean Varieties Contributes to Clarify Cultivated Olive Diversification

Olive tree with its main final product, olive oil, is an important element of Mediterranean history, considered the emblematic fruit of a civilization. Despite its wide diffusion and economic and cultural importance, its evolutionary and phylogenetic history is still difficult to clarify. As part of the Mediterranean basin, Algeria was indicated as a secondary diversification center. However, genetic characterization studies from Maghreb area, are currently underrepresented. In this context, we characterized 119 endemic Algerian accessions by using 12 microsatellite markers with the main goal to evaluate the genetic diversity and population structure. In order to provide new insights about …

The effect of different vigour olive clones on growth, dry matter partitioning and gas exchange under water deficit

Abstract In the last decade, high-density olive planting systems have been developed to facilitate less costly mechanical harvesting and pruning by using straddle harvesters. High-density orchards allow faster production since the first crop of fruit starts a few years after planting. In high-density orchards the control of tree size is essential. It is possible to control the vigour and size of the tree by selecting suitable cultivars, by choosing rootstocks capable of controlling scion vigour and, finally, by applying deficit irrigation to reduce plant vegetative growth. Knowledge of tree water requirements is needed for precise irrigation scheduling. In this study, the dwarfing capacity …

INTRA-CULTIVAR DIVERSITY IN SOUTHERN ITALY OLIVE CULTIVARS DEPICTED BY MORPHOLOGICAL TRAITS AND SSR MARKERS

In this study, SSR analysis was performed on a large number of putative clones of the main olive cultivars from two Italian regions, Sicily and Calabria, in order to study the genetic relatedness, and to detect any degree of genetic diversity, which can be used to unambiguously identify possible superior clones. These putative clones have been collected and observed in the last ten years, following an extensive investigation carried out in traditional olive growing areas, thanks to the reports of farmers, millers, technician, growers and nurseries. The set of microsatellite (SSR) markers used allowed the detection of the genetic diversity, resulting from somatic mutation, indicating the pre…

Detecting Mild Water Stress in Olive with Multiple Plant-Based Continuous Sensors.

A comprehensive characterization of water stress is needed for the development of automated irrigation protocols aiming to increase olive orchard environmental and economical sustainability. The main aim of this study is to determine whether a combination of continuous leaf turgor, fruit growth, and sap flow responses improves the detection of mild water stress in two olive cultivars characterized by different responses to water stress. The sensitivity of the tested indicators to mild stress depended on the main mechanisms that each cultivar uses to cope with water deficit. One cultivar showed pronounced day to day changes in leaf turgor and fruit relative growth rate in response to water w…

Canopy Architecture Appraisal by Fractal Dimension of 'Flordastar' Peach Trees Grafted onto Different Rootstocks

The objective of this research was to evaluate the modification of canopy architecture of ''Flordastar'' peach (Prunus persica L. Batsch) grafted onto rootstocks with different vigour, by the use of fractal dimension (D). The hypothesis was that different vigour rootstocks are able to modify the complexity of the branching pattern and that this effect can be assessed by a geometric parameter such as the fractal dimension (D) of the 2D projection of tree branching structure. The observations were carried out in a four-year-old experimental orchard of cv. ''Flordastar'' peach trees grafted onto Ishtara, Barrier, GF677 and MrS 2/5 rootstocks. On digital pictures of leafless, dormant peach tree…

Growth and physiological responses of young olive trees affected by Olive leaf yellowing associated virus

We studied the effect of Olive leaf yellowing associated virus (OLYaV) on biometric and physiological changes in olive. The trial was carried out on 32 two-yearold virus-free trees of 'Frantoio' grafted on virus-free olive seedlings and grown in 32-L pots. Half of the trees were inoculated with OLYaV in the fall of 2007. At 6, 18, and 24 months after inoculation, tree height, basal trunk diameter and total leaf number were determined, and leaf area tree-1 was estimated from a 50-leaf subsample. Twigs of various order were also counted and measured. At the end of the experiment, dry matter partitioning, leaf gas exchange and water potential were also measured. Total twig length and number, t…

Effect of planting system on productivity, dry-matter partitioning and carbohydrate content in aboveground components of 'Flordaprince' peach trees

Vegetative growth, fruit yields, and dry matter partitioning within above-ground components were assessed during three growing seasons for trees of an early ripening peach (Prunus persica L. Batsch `Flordaprince' on GF 677 rootstock) trained either to a free standing central leader (930 trees/ha) or to Y shape (1850 trees/ha). Individual trees trained to central leader gave higher fruit yield, had a significantly greater leaf area and accumulated more dry mass in above-ground components per tree than Y shape trees. The training systems did not differ in terms of yield efficiency (yield per trunk cross-sectional area) and leaf area index (LAI), but Y shape trees had a higher harvest index an…

Biomass and volume modeling in Olea europaea L. cv "Leccino"

Key message: This work demonstrates that the Olive tree, which is managed and pruned as a fruit tree, can be treated as a forest tree using allometric equations, to estimate both biomass production and volumes. Abstract: The Olive tree (Olea europaea L.) is an evergreen tree that can grow and accumulate a relatively high amount of dry matter, even in dry environmental conditions common in the Mediterranean basin and typical of traditional rain-fed agriculture. The objective of this research was to develop a tool to predict woody biomass and tree component volume for the olive tree, to be used for different agricultural and environmental purposes. The study was carried out in six olive grove…

Gaining Insight into Exclusive and Common Transcriptomic Features Linked to Drought and Salinity Responses across Fruit Tree Crops

The present study aimed at identifying and mapping key genes expressed in root tissues involved in drought and salinity tolerance/resistance conserved among different fruit tree species. Twenty-six RNA-Seq samples were analyzed from six published studies in five plant species (Olea europaea, Vitis riparia Michx, Prunus mahaleb, Prunus persica, Phoenix dactylifera). This meta-analysis used a bioinformatic pipeline identifying 750 genes that were commonly modulated in three salinity studies and 683 genes that were commonly regulated among three drought studies, implying their conserved role in resistance/tolerance/response to these environmental stresses. A comparison was done on the genes th…

Development of a Sensor for Continuous and Accurate Monitoring of Air Flow for Open-System Whole Canopy Gas-Exchange Measurements

The research describes the development and set-up of a simple and accurate solid-state device for continuous monitoring of airflow forced into an open-flow gas-exchange chamber for whole-canopy transpiration and photosynthesis measurements. The ventilation system was formed by a centrifugal fan powered by an electric motor and a PVC pipe (0.193 m inner diameter). To damp airflow turbulence, 50 cm of the main pipe was filled with a bundle of smaller PVC pipes (3 cm inner diameter). The measurement principle of the sensor is the thermal balance of an electrically heated copper plate compared to an unheated one. Calibration of the sensor was performed by two independent methods: gas dilution (…

Automatic detection and agronomic characterization of olive groves using high-resolution imagery and LIDAR data

The Common Agricultural Policy of the European Union grants subsidies for olive production. Areas of intensified olive farming will be of major importance for the increasing demand for oil production of the next decades, and countries with a high ratio of intensively and super-intensively managed olive groves will be more competitive than others, since they are able to reduce production costs. It can be estimated that about 25-40% of the Sicilian oliviculture must be defined as “marginal”. Modern olive cultivation systems, which permit the mechanization of pruning and harvest operations, are limited. Agronomists, landscape planners, policy decision-makers and other professionals have a grow…

Improvement in yield and fruit size and quality of the main Italian table olive cultivar 'Nocellara del Belice'

Olive (Olea europaea L.) shows alternate bearing, with unreliable cropping patterns and inconsistent fruit size and quality every year. In many countries, thinning with naphthaleneacetic acid (NAA) is used to minimise alternate bearing behavior in olives. However, in Italy, growers are reluctant to employ this practice and no detailed knowledge is available for specific cultivars. We evaluated the effects of spraying NAA on various dates on the productive and vegetative characteristics of the main Italian table olive cultivar'Nocellara del Belice'. Trunk cross-sectional area, fruit set, fruit drop, fruit size, pit size, yield per tree, crop density and flesh to pit ratio were analysed. The …

Gas Exchanges and Stem Water Potential Define Stress Thresholds for Efficient Irrigation Management in Olive (Olea europea L.)

With climate change and decreased water supplies, interest in irrigation scheduling based on plant water status is increasing. Stem water potential (ΨSWP) thresholds for irrigation scheduling in olive have been proposed, however, a physiologically-based evaluation of their reliability is needed. A large dataset collected at variable environmental conditions, growing systems, and genotypes was used to characterize the relation between ΨSWP and gas exchanges for olive. Based on the effect of drought stress on the ecophysiological parameters monitored, we described three levels of stress: no stress (ΨSWP above about −2 MPa), where the high variability of stomatal conductance (gs) suggests a ti…

Seasonal changes in starch content in pistachio organs as related to crop load

The alternate bearing in pistachio (Pistacia vera L.) results from inflorescence buds abscission differentiated on current season growth during the year of heavy crop. Inflorescence bud drop has been directly correlated with the presence of fruits on 1-year shoot, and in particular with the kernel grow that acts as the major sink of nutrients. Experimental evidences suggest the involvement of competition between reproductive organs for the available resources. The annual carbohydrates storage was analysed in mature pistachio trees characterized by low, medium and high crop load (about 1, 7 and 11 kg tree(-1) of dry in-shell nuts). The experiment was carried out in 2013 in a commercial pista…

A carbon budget model to predict branch carbohydrate deficiencies as a function of water stress and crop load in pistachio (Pistacia veraL.)

The effect of irrigation and crop load on alternate bearing was studied in pistachio tree using a “branch carbon budget model” developed to calculate branch carbohydrate balance. Experiences were conducted in Sicily (37°26’N, 14°03’E, 360 m a.s.l.) on female trees of ‘Bianca’. Two treatments were applied: rainfed (T0) and 100 mm of irrigation (T100). At 29, 44, 65, 86, 103 and 121 DAFB, on one fully expanded leaf selected on three trees per treatment, were monitored leaf gas exchanges. At 15 days interval, three branches treatment-1 were excised and, in the lab, the following parameters were measured: total photosynthetically active leaf surface; number of leaves, fruits, shoot fresh weight…

Deciphering transcriptional regulation mechanisms underlining fruit development and ripening in Vitis vinifera

BACKGROUND: Grapes (Vitis vinifera ) are an important woody crop cultivated in a broad range of environmental conditions. Grapefruit development is a physiological process whose molecular regulatory networks are still not sufficiently investigated. OBJECTIVE: The primary aim of the work was to identify which key genes, molecular mechanisms and networks were involved in fruit ripening and development through a comparison of available transcriptomic data at different stages during grape development and ripening. Secondly, we aimed at identifying among these fruit-related genes, which genes play also a functional role in other developmental and physiological processes in reproductive tissues (…

Genetic Improvement of Sweet Chestnut in Sicily (Castanea sativa Mill.) by the Selection of Superior Autochthonous Genotypes

In Sicily, the chestnut (Castanea sativa Mill.) grows in limited areas of the South eastern Mt. Etna volcano and of the Northern Mountains, namely Madonie and Nebrodi. In the Island, chestnut can be considered as a neglected species, because of the modest importance recognized to it for horticultural and forestry exploitation. Recently, the Sicilian regional government founded the 'Horticultural Forestry' project to promote the cultivation of chestnut as possible fruit tree for local city farmer markets, where typical products are usually sold. The project was developed in five steps, carried out during three years by the Dipartimento di Colture Arboree of Palermo University: identification…

The Effect of Plant Water Status on the Chemical Composition of Pistachio Nuts (Pistacia vera L. Cultivar Bianca)

Pistachio nuts are worldwide appreciated for their chemical and organoleptic profiles. There are several studies on the influence of irrigation on pistachio productivity, whereas there are little available data on the influence on nut quality. In this study we characterized some qualitative traits of pistachio nuts cultivar Bianca in Mediterranean environment and how plant water status affected them. Water status had a positive and significant influence on the chlorophylls content, nuts from less stressed trees showed higher values of chlorophyll a (14.7 mg/100 g) and b (21.1 mg/100 g) compared than more stressed trees (9.3 and 11.5 mg/100 g for a and b, respectively). Solid phase microextr…

The first high-density sequence characterized SNP-based linkage map of olive (Olea europaea L. subsp. europaea) developed using genotyping by sequencing

A number of linkage maps have been previously developed in olive; however, these are mostly composed of markers that have not been characterized at the sequence level, supplemented with smaller numbers of microsatellite markers. In this investigation, we sought to develop a saturated linkage mapping resource for olive composed entirely of sequence characterized markers. We employed genotyping by sequencing to develop a map of a F2 population derived from the selfing of the cultivar Koroneiki. The linkage map contained a total of 23 linkage groups comprised of 1,597 tagged SNP markers in 636 mapping bins spanning a genetic distance of 1189.7 cM. An additional 6,658 segregating SNPs were asso…

RECOVERY AND CHARACTERIZATION OF THE CHESTNUT GERMPLASM ON THE WESTERN SLOPES OF THE ASPROMONTE IN SOUTHERN CALABRIA

For a considerable period of time chestnuts were a fundamental source of food and economy for the people in the Aspromonte area. After decades in which chestnut cultivation has been neglected as a result of depopulation in mountainous zones, the production capacity in this area has reduced resulting in an erosion of the native germplasm. A survey has been carried out to improve the knowledge about the chestnut germplasm present in this area and to evaluate its qualities with the objective of preserving native genetic resources, safeguarding them from genetic erosion phenomenon and individuating local genotypes of market value which could be used to promote a re-launch of local chestnut cult…

Two new planting systems for early ripening peaches (Prunus persica L. Batsch): Yield and fruit quality in four low-chill cultivars

SummaryTrees of peach cvs Flordastar, Flordaprince, Flordacrest and Maravilha, low in chill requirement (Flordaprince 150; Flordacrest 350 C.U.), with a short fruit development period (Flordaprince 90; Flordacrest 110 d) and a very early ripening time (Flordastar mid May; Flordacrest first week of June), were planted in Sicily (37° 30′ Lat. N) both in a medium (667 trees per ha) and a high density (2000 trees per ha) plantation system. In the medium density planting system, the trees were trained to a Fusetto, and in the high density to a Y-shape. Both in the Fusetto and Y, the size of the trees was controlled by severe pruning soon after harvest, consisting in the shortening of most of the…

Validation of an online system for the continuous monitoring of tree water status for sustainable irrigation managements in olive (Olea europaea L.)

Abstract As a result of climate change a large reduction of agricultural water through improved irrigation management is a major need for agriculture sustainability. To this aim, always more sensitive sensors to monitor plant water status have been developed in recent years. Among them, the leaf patch clamp pressure probes are very promising for water management of olive but until now have been tested only in few environmental and management conditions. In this work these sensors have been tested for two consecutive years on two completely different management systems: a traditional rainfed orchard and a super high density (SHD) drip irrigated orchard. Within the SHD orchard the probes have…

Study of energetic properties of different tree organs in six Olea europaea L. cultivars

AbstractPruning is an important horticultural practice for the management of olive orchards (Olea europaea L.) that generates a considerable amount of residues every year. Olive orchards are increasingly expanding beyond the Mediterranean Basin to new growing Countries (Australia, California, Argentina, Chile and Uruguay) and this will certainly lead to larger availability of pruning material. Currently, the interest in use of olive tree pruning residues for energy purposes is increasing but unfortunately, the information on the differences among organs of the tree, in terms of calorific value and ash content, is scarce. Another unknown aspect is the effect of cultivar vigour on dry matter …

Yield and profitability of modified spanish bush and Y-trellis training systems for peach

Peach trees trained to modified Spanish bush (MSB) and Y-trellis (Y) were evaluated and compared in the Mediterranean settings of Southern Italy. The observations included two peach (Rich May and Summer Rich) and two nectarine (Big Bang and Nectaross) cultivars. In the MSB system, trees were spaced at 4.5 x 2.5 m (888 trees/ha), whereas in the Y system, trees were spaced at 5.5 x 2 m (909 trees/ha). Costs at planting, yield per tree, fruit size grade, unit price of sold peaches for each size grade, amount and cost of materials and labor for cultural management, and grower's profit were quantified throughout all six years from planting and at full crop (year 4-6). Fixed costs of the MSB syst…

Morphological and molecular variability within the fig cultivar 'Dottato' in the Italian protected designation origin area "fichi di Cosenza"

The morphological and molecular diversity among fig accessions of 'Dottato', found in the PDO "Fichi di Cosenza" area was studied by evaluating 24 morphological traits and by genotyping with 18 microsatellite markers. The microsatellite allelic profiles among the putative clones of 'Dottato' indicated a moderate genetic variability, discriminating unambiguously most of the accessions. Only two groups of identity were found. The average expected and observed heterozygosity were 0.43 and 0.62, respectively. The mean polymorphic information content (PIC) was 0.4, varying from 0.08 (LMFC26) to 0.067 (FCUP 38-6). The morphological clustering allowed the distinction of all genotypes. Some genotyp…

In-Field and Early Detection of Xylella fastidiosa Infections in Olive Using a Portable Instrument

Xylella fastidiosa subsp. pauca (Xfp) is a gram-negative pathogenic bacteria responsible for serious diseases (Purcell, 2013) that inflicts considerable economic loss (Li et al., 2007; Luvisi et al., 2017). The pathogen has been linked to olive quick decline syndrome (OQDS). This devastating olive disease was first observed in Salento (Apulia, southeastern Italy) in 2009. Infected trees respond to Xfp infection with scattered desiccation of twigs and small branches in the upper crown, which extend to the rest of the canopy, showing the characteristic blight effect. The disease causes tree death within a few years from the onset of symptoms (Martelli, 2016). The primary agronomic procedure f…

Molecular and morphological diversity of on-farm hazelnut (Corylus avellana L.) landraces from southern Europe and their role in the origin and diffusion of cultivated germplasm

Hazelnut (Corylus avellana L.) is a traditional nut crop in southern Europe. Germplasm exploration conducted on-farm in five countries (Portugal, Spain, Italy, Slovenia, and Greece) identified 77 landraces. The present work describes phenotypic variation in nut and husk traits and investigates genetic relationships using ten simple sequence repeat (SSR) markers among these landraces, 57 well-known references cultivars, and 19 wild accessions. Among the 77 landraces, 42 had unique fingerprints while 35 showed a SSR profile identical to a known cultivar. Among the 42 unique landraces, morphological observations revealed high phenotypic diversity, and some had characteristics appreciated by th…

Evaluation of morphological and genetic diversity of loquat accessions grown in sicily

In this study 20 accessions of loquat (Eriobotrya japonica Lindl.) that originated from Sicily and other countries were characterized phenotypically and genotyped using seven microsatellite markers. The microsatellites clearly distinguished 14 accessions; three pairs of local accessions were not differentiated, and three accessions from abroad can be considered bud sport mutations of the main Spanish cultivar ‘Algerie’. The mean alleles per locus was four, ranging from two with the marker EJ075 and five with EJ012 and EJ271; the mean Polymorphic Information Content was 0.53; the average expected and observed heterozygosities resulted 0.62 and 0.59, respectively. These values revealed a …

LOW VIGOR PEACH × ALMOND ROOTSTOCKS FOR INTENSIVE PEACH PLANTINGS IN MEDITERRANEAN ENVIRONMENTS

A three-year trial was carried out to evaluate the vegetative and productive performance of five peach rootstocks of varied vigor. The study was conducted in south-western Sicily using 45 V-trained trees of 'Tropic Snow' peach planted in 2005 and grafted on GF677 and four low-vigor rootstocks selected at the Department of Agricultural, Food and Environmental Sciences, University of Pisa, namely IS5/8 (Polluce), IS5/19 (Castore), IS5/23, IS5/29. Trunk circumference, winter and summer pruning weight, number and mass of fruit were recorded from 2010 to 2012. All IS selections reduced trunk cross-sectional area (TCSA) and pruning weight compared to GF677 with IS5/23 inducing the highest reducti…