Chromatographic monitoring of diuretics in urine samples using a sodium dodecyl sulphate—propanol micellar eluent

Abstract The effect of a varying pH, in the range 3−7, on the retention of several diuretics eluted with a sodium dodecyl sulphate (SDS) micellar mobile phase and with UV detection was studied. Significant changes in the capacity factors ( K′ of the diuretics bumetanide, ethacrynic acid, furosemide, probenecid and xipamide were observed. The protonation constants of these compounds were calculated from the k′ data. A study was also performed to select the optimum composition of the mobile phase (pH, concentration of SDS and nature and concentration of alcohol) for the separation of the above diuretics and the diuretics amiloride, bendroflumethiazide, chlorthalidone, hydrochlorothiazide, spi…