6533b7d2fe1ef96bd125e015
RESEARCH PRODUCT
Chromatographic monitoring of diuretics in urine samples using a sodium dodecyl sulphate—propanol micellar eluent
E. Bonet-domingoJ.r. Torres-lapasio′oM.j. Medina-hern′andezM.c. Garc′ia-alvarez-coquesubject
TriamtereneChromatographySodiummedicine.medical_treatmentchemistry.chemical_elementBiochemistryAnalytical ChemistryAmilorideHydrochlorothiazidechemistryMicellar solutionsmedicineEnvironmental ChemistryBendroflumethiazideXipamideDiureticSpectroscopymedicine.drugdescription
Abstract The effect of a varying pH, in the range 3−7, on the retention of several diuretics eluted with a sodium dodecyl sulphate (SDS) micellar mobile phase and with UV detection was studied. Significant changes in the capacity factors ( K′ of the diuretics bumetanide, ethacrynic acid, furosemide, probenecid and xipamide were observed. The protonation constants of these compounds were calculated from the k′ data. A study was also performed to select the optimum composition of the mobile phase (pH, concentration of SDS and nature and concentration of alcohol) for the separation of the above diuretics and the diuretics amiloride, bendroflumethiazide, chlorthalidone, hydrochlorothiazide, spironolactone and triamterene. Maximum resolution was achieved with a 0.042 M SdS+4.0% propanal eluent. Limits of detection (at 254 nm) ranged between 0.082 μml −1 for xipamide and 0.76 μml −1 for ethacrynic acid. The method was applied to the study of the urinary excretion of several diuretics.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-03-01 | Analytica Chimica Acta |