Characterization of the Carbapenem-Hydrolyzing Oxacillinase Oxa-58 in an Acinetobacter Genospecies 3 Clinical Isolate
ABSTRACT Based on imipenem resistance in an Acinetobacter genospecies 3 clinical isolate, we were able to identify, for the first time in this genomic species, a plasmid-encoded bla OXA-58 gene that was 100% homologous to the same gene in Acinetobacter baumannii .
Diagnóstico microbiológico de las infecciones gastrointestinales
Resumen Las infecciones agudas del tracto gastrointestinal figuran entre las enfermedades infecciosas más frecuentes. En esta revisión se examinan diversas técnicas para diagnosticar las gastroenteritis que ocasionan bacterias, virus y parásitos. El coprocultivo es el método de elección para el diagnóstico de las infecciones bacterianas intestinales, aunque las infecciones por Clostridium difficile se pueden diagnosticar mediante la detección de las toxinas A y B en las heces y las infecciones por Escherichia coli diarreagénicas se pueden diagnosticar mediante la detección por reacción en cadena de la polimerasa de factores de virulencia específicos de los diversos enteropatotipos. Las técn…
Fundamentos e implementación de Programas de Optimización de Diagnóstico Microbiológico
Resumen Los programas de optimizacion del diagnostico microbiologico (PRODIM) promueven actuaciones coordinadas orientadas a optimizar el uso de las tecnicas diagnosticas, propiciando la toma de decisiones terapeuticas, clinicas y preventivas adecuadas y «coste-efectivas». La implementacion de los PRODIM debe sustentarse en la creacion de comites multidisciplinares liderados por microbiologos clinicos para el diseno de algoritmos diagnosticos, la adecuacion del sistema informatico del laboratorio para el control de la pertinencia de las pruebas diagnosticas solicitadas, la puesta en marcha de un sistema de control de calidad, el diseno y realizacion de estudios de analisis de coste-efectivi…
Fundamentals and implementation of Microbiological Diagnostic Stewardship Programs.
Microbiological diagnostic stewardship programs promote coordinated measures aimed at optimizing the use of diagnostic techniques, thus favouring the adoption of adequate and cost-effective therapeutic, clinical and preventive decisions. The implementation of microbiological diagnostic stewardship relies upon the creation of multidisciplinary committees led by clinical microbiologists for the design of diagnostic algorithms, the adequacy of the laboratory computer system to monitor the relevance of the requested diagnostic tests, the implementation of a quality control system, the design and performance of studies of cost-effectiveness, the training of the petitioner and the technical and n…
Characterization of the carbapenem-hydrolyzing oxacillinase OXA-58 in an Acinetobacter phenon 6/ct13TU clinical isolate
The bla(OXA-58) gene identified in the Acinetobacter phenon 6/ct13TU clinical isolate presented 100% homology with the same gene in Acinetobacter baumannii. Its location in a plasmid suggests that these resistance genes may be transferred from 1 species to another.
Plasmid-mediated QnrS2 determinant from a clinical Aeromonas veronii isolate.
The main objective of this study was to determine the prevalence of the Qnr determinants in clinical and environmental Aeromonas spp. A total of 52 Aeromonas sp. isolates identified by biochemical methods (5), 25 isolated from natural waters (1) and 27 isolated from clinical samples from hospitals in Valencia, Spain, were tested for quinolone resistance by the disk diffusion method (4) (nalidixic acid, 30 μg; oxolinic acid, 2 μg; flumequine, 30 μg; ciprofloxacin, 5 μg; and levofloxacin, 5 μg). Among the studied isolates, 27 showed resistance to nalidixic acid and susceptibility to ciprofloxacin, 24 isolates were susceptible to both nalidixic acid and ciprofloxacin, and only 1, the A. veroni…