0000000000178132
AUTHOR
Andreas Leunig
Aufnahme und Phototoxizität von Hämatoporphyrinderivat in normalen und malignen Zellen
Die Photodynamische Therapie (PDT) stellt neben der Chemo-, Radio- und chirurgischen Therapie ein neues alternatives Therapiekonzept in der Behandlung von malignen Tumoren dar. Das Prinzip beruht auf der systemischen Applikation (i.v. Injektion) einer photosensibilisierenden Substanz, z.B. Hamatoporphyrinderivat (HPD), und der nachfolgenden Bestrahlung mit Licht geeigneter Wellenlange, z.B. Laserlicht (630 nm). Fur die tumorselektive Wirkung dieser Therapie werden die selektive Aufnahme bzw. vermehrte Speicherung der photoaktiven Substanzen im Tumorgewebe [1] sowie auch eine im Vergleich zum Normalgewebe erhohte Sensibuitat von Tumorgewebe gegenuber der PDT postuliert. Ungeklart ist, ob die…
An in vitro model to study cellular photosensitizer uptake and photodynamic dose-response relationships of tumor cells
Cellular fluorescence intensity (CFI) after incubation with varying concentrations of the photosensitizer Photofrin and the photodynamically induced dose-response relationships of hamster melanoma cells (A-MEL-3) were studied in a recently developed in vitro model. After administration of Photofrin to the extracellular serum-free medium, CFI was evaluated by flow cytometry together with constantly fluorescing latex particles used as a reference. After 5 min, 50% of maximal CFI was found, and after 60 min CFI was maximal. No further increase was obtained during the exposure to Photofrin over the incubation period of 4 h. During this plateau phase, CFI was significantly related to the concent…
Relation of early Photofrin uptake to photodynamically induced phototoxicity and changes of cell volume in different cell lines.
For efficacy of photodynamic therapy, selective uptake and retention of photoactive substances has been postulated. Therefore, measurements were performed to find out whether the photosensitiser Photofrin® is taken up differently in malignant and non-malignant cells in vitro . In addition, the sensitivity of malignant cells and nonmalignant cells to photodynamic exposure was investigated, by quantifying viability and volume alterations of the cells. Bovine aortic endothelial cells, mouse fibroblasts and amelanotic hamster melanoma cells were suspended in a specially designed incubation chamber under controlled conditions (e.g. pH, p O 2 , p CO 2 and temperature). After establishing constant…
Real-life assessment of chronic rhinosinusitis patients using mobile technology: the mySinusitisCoach project by EUFOREA
Abstract Background Chronic rhinosinusitis (CRS) is a chronic inflammatory disease associated with a substantial personal and socioeconomic burden. Monitoring of patient‐reported outcomes by mobile technology offers the possibility to better understand real‐life burden of CRS. Methods This study reports on the cross‐sectional evaluation of data of 626 users of mySinusitisCoach (mSC), a mobile application for CRS patients. Patient characteristics of mSC users were analysed as well as the level of disease control based on VAS global rhinosinusitis symptom score and adapted EPOS criteria. Results The mSC cohort represents a heterogeneous group of CRS patients with a diverse pattern of major sy…
In-vitro Untersuchungen zu photodynamisch induzierten Dosis-Wirkungs-Beziehungen
Im Gegensatz zu fruheren Annahmen wurde von Rodgers 1990 in Frage gestellt, das aus der Quantifizierung der Fluoreszenz im Gewebe Aussagen uber die Konzentration des Photosensibilisators noch uber die zu erwartenden photodynami-schen Effekte moglich sind (1).