0000000000179026
AUTHOR
V.p. Patyka
Soil microbial activity and functional diversity in primeval beech forests
Virgin forests are unique ecosystems, which can be used as etalon for basic biocoenotic investigation. Soil microorganisms are very sensitive reagents on influence of biotical factors, and at the same time are the active producers of phytotoxic and phytostimulating exometabolites. Studies of soil microbiota were conducted in virgin beech forests of Shyrokoluzhansky massif of the Carpathian Biosphere Reserve. It was found the ratio and the number of different ecological-trophic groups of soil microorganisms changes with altitude. So the number of ammonificators with increasing of altitude above sea level was reduced. The soil at altitude of 1,100 meters above sea level was characterized by m…
Identification of phytopathogenic bacteria in maize seeds in Ukraine
The main bacterial diseases of maize: bacterial wilt of maize (Pantoea stewartii subsp. stewartii), goss’s bacterial wilt (Clavibacter michiganensis subsp. nebraskensis), seed rot-seedling blight of maize (Bacillus subtilis), bacterial spot of maize (Pseudomonas syringae pv. syringae), bacterial leaf spot of maize (Pantoea agglomerans) and bacterial stalk rot of maize caused by several causative agents, were described. The causative agents of these diseases are often stored and transmitted with seeds, so seed analysis for the presence of phytopathogenic bacteria is an important step in the pathogen control system. For analysis, the corn seeds that were grown in the Poltava and Kiev regions …
Specifics of pesticides effects on the phytopathogenic bacteria
Abstract The data concerning the effects of pesticides of different nature on the phytopathogenic bacteria was examined and summarized. Without extensive research on the mechanisms of interaction between pathogenic bacteria and pesticides in the literature review a similar message about microorganisms of soil and phyllosphere are included. The bacteria can be suppressed permanently by pesticides with a mechanism of action that universally affects biological processes in living systems. Long-term storage, ease of use and fast visible effect are the advantages of synthetic pesticides remedies. But chemical pollution, shifts in the balance of ecosystems, unpredictable effects of chemical pesti…
Influence of citrates nanoparticles on morphological traits of bacterial cells pseudomonas syringae pv. atrofaciens
Electron microscope investigations have revealed disintegration of the cell wall of Pseudomonas syringae pv. atrofaciens and subsequent killing of bacterial cells after application of 1% solutions of the citrates of Ag-Cu nanoparticles, the complex Co-Cu-Zn-Fe-Mn-Mo-Mg (Avatar-1) and iodine-selenium. In field conditions, it was revealed that pre-sowing treatment of wheat seeds with the aforementioned 1% solutions significantly inhibited the development of basal glume rot in cereals with artificial infection of Pseudomonas syringae рv. аtrofaciens. Consequently, reduction of the typical symptoms of basal glume rot of cereals and the percentage of isolated cells of Pseudomonas syringae рv. аt…
Characteristic of Pseudomonas syringae pv. atrofaciens Isolated from Weeds of Wheat Field
The aim of this study was the identification of the causative agent of the basal glume rot of wheat Pseudomonas syringae pv. atrofaciens from the affected weeds in wheat crops, and determination of its virulent properties. Isolation of P. syringae pv. atrofaciens from weeds of wheat crops was carried out by classical microbiological methods. To identify isolated bacteria, their morphological, cultural, biochemical, and serological properties as well as fatty acids and Random Amplification of Polymorphic DNA (RAPD)-PCR (Polymerase chain reaction) profiles with the OPA-13 primer were studied. Pathogenic properties were investigated by artificial inoculation of wheat plants and weed plants, fr…
ENHANCEMENT OF WHEAT VIRUS-RESISTANCE AT APPLICATION OF THE SE NANOPARTICLES CITRATES AND CONSORTIUM OF SOIL MICROORGANISMS
The influence of pre-sowing seed treatments of selenium nanocitrates (SeNPs) and application of soil formed microorganism consortium (biological preparation (BP) Extrakon) on wheat plants infected with wheat streak mosaic virus (WSMV) were investigated in greenhouse and fields conditions in 2018-2019 (on territories of Zabolotny Institute of Microbiology and Virology of NASU). The pre-sowing seed treatments of 1% selenium nanocitrates (SeNPs) with application of BP Extrakon initiating the growth of juvenile wheat in laboratory experiments were found. In the field experiment on variants of WSMV-infected plants, two weeks after inoculation (tillering stage), we observed the appearance of char…