6533b861fe1ef96bd12c5a87
RESEARCH PRODUCT
Characteristic of Pseudomonas syringae pv. atrofaciens Isolated from Weeds of Wheat Field
Yuliia KolomiietsDariusz SuszanowiczAntonina KalinichenkoMonika SporekLidiia PasichnykLiudmyla ButsenkoV.p. Patykasubject
0301 basic medicine030106 microbiologyVirulencelcsh:Technologyfatty acidslaw.inventionlcsh:Chemistry<i>Pseudomonas syringae</i> pv. <i>atrofaciens</i>03 medical and health scienceslawRAPDwheatBotanyPseudomonas syringaeweedsbasal glume rotGeneral Materials ScienceInstrumentationlcsh:QH301-705.5Polymerase chain reactionFluid Flow and Transfer ProcessesPseudomonas syringae pv.atrofaciensbiologyInoculationlcsh:TProcess Chemistry and TechnologyGlumefungiGeneral Engineeringfood and beveragesphenotypic and genotypic propertiesbiology.organism_classificationlcsh:QC1-999Computer Science ApplicationsRAPD030104 developmental biologylcsh:Biology (General)lcsh:QD1-999lcsh:TA1-2040Weedlcsh:Engineering (General). Civil engineering (General)Bacterialcsh:Physicsdescription
The aim of this study was the identification of the causative agent of the basal glume rot of wheat Pseudomonas syringae pv. atrofaciens from the affected weeds in wheat crops, and determination of its virulent properties. Isolation of P. syringae pv. atrofaciens from weeds of wheat crops was carried out by classical microbiological methods. To identify isolated bacteria, their morphological, cultural, biochemical, and serological properties as well as fatty acids and Random Amplification of Polymorphic DNA (RAPD)-PCR (Polymerase chain reaction) profiles with the OPA-13 primer were studied. Pathogenic properties were investigated by artificial inoculation of wheat plants and weed plants, from which bacteria were isolated. For the first time, bacteria that are virulent both for weeds and wheat were isolated from weeds growing in wheat crops. It was shown that the fatty acids profiles of the bacteria isolated from the weeds contained typical for P. syringae pv. atrofaciens fatty acids, in particular, hydroxy acids: 3-hydroxydecanoic, 2-hydroxydodecanoic, and 3-hydroxydodecanoic. RAPD-PCR profiles of the newly isolated strains were identical to those of the collection strains P. syringae pv. atrofaciens UCM B-1011 and P. syringae pv. atrofaciens UCM B-1014 and contained a dominant fragment of 700 bp. The isolated strains, according to their phenotypic and genotypic properties, were identified as P. syringae pv. atrofaciens. It was established that the causative agent of basal glume rot of wheat P. syringae pv. atrofaciens is polyphagous and capable of infecting a wide range of plants. The main control measure for cereals diseases caused by P. syringae pv. Atrofaciens&mdash
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-12-01 | Applied Sciences |