Identification of phytopathogenic bacteria in maize seeds in Ukraine
The main bacterial diseases of maize: bacterial wilt of maize (Pantoea stewartii subsp. stewartii), goss’s bacterial wilt (Clavibacter michiganensis subsp. nebraskensis), seed rot-seedling blight of maize (Bacillus subtilis), bacterial spot of maize (Pseudomonas syringae pv. syringae), bacterial leaf spot of maize (Pantoea agglomerans) and bacterial stalk rot of maize caused by several causative agents, were described. The causative agents of these diseases are often stored and transmitted with seeds, so seed analysis for the presence of phytopathogenic bacteria is an important step in the pathogen control system. For analysis, the corn seeds that were grown in the Poltava and Kiev regions …
The Effect of Pesticides on the Tomato Bacterial Speck Disease Pathogen Pseudomonas Syringae pv. Tomato
A significant part of the used pesticides does not reach the target organisms and, while remaining in the agrophytocenosis, influences all living organisms in it. Having a toxic and often mutagenic effect, pesticides induce morphological and physiological changes in the cells of microorganisms and are the cause of phenotypic heterogeneity of their populations. However, the effect of pesticides on phytopathogenic bacteria as non-target microorganisms remains out of the field of view for most researchers. However, the use of pesticides can lead to expansion of the diversity of existing phytopathogens and, as a consequence, complications of identification of the pathogens, loss of resistance b…
Specifics of pesticides effects on the phytopathogenic bacteria
Abstract The data concerning the effects of pesticides of different nature on the phytopathogenic bacteria was examined and summarized. Without extensive research on the mechanisms of interaction between pathogenic bacteria and pesticides in the literature review a similar message about microorganisms of soil and phyllosphere are included. The bacteria can be suppressed permanently by pesticides with a mechanism of action that universally affects biological processes in living systems. Long-term storage, ease of use and fast visible effect are the advantages of synthetic pesticides remedies. But chemical pollution, shifts in the balance of ecosystems, unpredictable effects of chemical pesti…
Bacterial Diseases of Bioenergy Woody Plants in Ukraine
In this study, the characterization of several bacterial diseases affecting silver birch (Betula pendula Roth.), common ash (Fraxinus excelsior L.), white poplar (Populus alba L.), and white willow (Salix alba L.) in Ukraine were described. The typical symptoms, features of pathogenesis, and characteristics of the causative agents of the most common bacterial diseases of these tree species were shown. The following types of bacterioses were noted to be especially dangerous, namely, bacterial wetwood, fire blight, bacterial canker, and tuberculosis. Bacterial necrosis of the bark was a less dangerous disease. At the same time, all of the listed types of bacterioses were registered within the…
Influence of citrates nanoparticles on morphological traits of bacterial cells pseudomonas syringae pv. atrofaciens
Electron microscope investigations have revealed disintegration of the cell wall of Pseudomonas syringae pv. atrofaciens and subsequent killing of bacterial cells after application of 1% solutions of the citrates of Ag-Cu nanoparticles, the complex Co-Cu-Zn-Fe-Mn-Mo-Mg (Avatar-1) and iodine-selenium. In field conditions, it was revealed that pre-sowing treatment of wheat seeds with the aforementioned 1% solutions significantly inhibited the development of basal glume rot in cereals with artificial infection of Pseudomonas syringae рv. аtrofaciens. Consequently, reduction of the typical symptoms of basal glume rot of cereals and the percentage of isolated cells of Pseudomonas syringae рv. аt…
Phytopathogenic Bacteria Associated with Bacterioses of Common Oak (Quercus robur L.) in Ukraine
Studying the biological characteristics of the causative agents of bacteriosis of the common oak (Quercus robur L.) (to highlight the links between the key factors of pathogenesis and bacterial groups) is a new and relevant area of research. This work aims to identify the species composition of phytopathogenic bacteria associated with common oak bacteriosis and to study the morphological, cultural, and biochemical properties of pathogens. To establish the general phytosanitary state of the surveyed stands, classical forest inventory and phytopathological (visual on based on typical symptoms of the disease and macroscopic) methods were used. The properties of isolated bacteria were studied u…
INFLUENCE OF CITRATE NANOPARTICLES ON PHOTOCHEMICAL ACTIVITY, RESISTANCE TO PATHOGENS AND PRODUCTIVITY OF WHEAT
In field experiments, increase the quantum efficiency of PSII (Fv/Fp), the Rfd-values (the chlorophyll fluorescence vitality indices) and the content of photosynthetic pigments in wheat leaves at pretreatment of wheat seeds with solutions of citrate nanoparticles has been shown. The green weight of plants at bacterial and phytoplasmas infecting at pretreatment with solutions of citrate nanoparticles, was increase, than without pre-treatment, in order: GeNPs>I-Se>SeNPs>VNPs as it has been shown. The Fv/Fp-values in leaves infected with phytoplasmas of plants at pretreatment of VNPs or I-Se solutions were more, than without pretreatment. The Fv/Fp-value in leafs with bacterial infecting at pr…
Characteristic of Pseudomonas syringae pv. atrofaciens Isolated from Weeds of Wheat Field
The aim of this study was the identification of the causative agent of the basal glume rot of wheat Pseudomonas syringae pv. atrofaciens from the affected weeds in wheat crops, and determination of its virulent properties. Isolation of P. syringae pv. atrofaciens from weeds of wheat crops was carried out by classical microbiological methods. To identify isolated bacteria, their morphological, cultural, biochemical, and serological properties as well as fatty acids and Random Amplification of Polymorphic DNA (RAPD)-PCR (Polymerase chain reaction) profiles with the OPA-13 primer were studied. Pathogenic properties were investigated by artificial inoculation of wheat plants and weed plants, fr…