Elucidating the catalytic reaction mechanism of orotate phosphoribosyltransferase by means of X-ray crystallography and computational simulations
15 p.-8 fig.-2 tab.1 graph. abst.+ 8 fig. supl.-1 tab. supl.
Use of neurofuzzy networks to improve wastewater flow-rate forecasting
A neurofuzzy wastewater flow-rate forecasting model (NFWFFM) has been developed and tested with actual data measured at the input of two wastewater treatment facilities which treat the wastewater corresponding to 150,000 and 1,250,000p.e., respectively. Good agreements between forecasted and actual flow-rates were obtained. The artificial intelligence algorithm uses only two input variables (day of the week and average daily flow-rate of day before) and one output variable (predicted average daily flow-rate). Using three months data for training the network, a long-term forecast (one month) is made with average errors below 10%. Results were compared with those obtained by applying the Cens…
[11C]-DASB microPET imaging in the aged rat: Frontal and meso-thalamic increases in serotonin transporter binding
Whereas molecular imaging studies in the aging human brain have predominantly demonstrated reductions in serotonin transporter (5-HTT) availability, the majority of the rodent studies, using autoradiographic methods, report increases in neural 5-HTT levels with age. To our knowledge, however, no previous rodent studies have assessed this topic in vivo, and therefore it remains unclear whether this discrepancy arises from methodological or inter-species differences. We performed an [(11)C]-DASB microPET study to evaluate the effects of aging on 5-HTT availability in the rat brain. To generate binding potential estimates, quantitative tracer kinetic modeling was applied using the simplified r…
Mechanism of sulfur transfer across protein-protein interfaces: The cysteine desulfurase model system
CsdA cysteine desulfurase (the sulfur donor) and the CsdE sulfur acceptor are involved in biological sulfur trafficking and in iron-sulfur cluster assembly in the model bacterium Escherichia coli. CsdA and CsdE form a stable complex through a polar interface that includes CsdA Cys328 and CsdE Cys61, the two residues known to be involved in the sulfur transfer reaction. Although mechanisms for the transfer of a sulfur moiety across protein-protein interfaces have been proposed based on the IscS-IscU and IscS-TusA structures, the flexibility of the catalytic cysteine loops involved has precluded a high resolution view of the active-site geometry and chemical environment for sulfur transfer. H…
In vivo molecular imaging of the GABA/benzodiazepine receptor complex in the aged rat brain
The GABA-ergic system, known to regulate neural tissue genesis during cortical development, has been postulated to play a role in cerebral aging processes. Using in vivo molecular imaging and voxel-wise quantification, we aimed to assess the effects of aging on the benzodiazepine (BDZ) recognition site of the GABA A receptor. To visualize BDZ site availability, [C-11]-flumazenil microPET acquisitions were conducted in young and old rats. The data were analyzed and region of interest analyses were applied to validate the voxel-wise approach. We observed decreased [C-11]-flumazenil binding in the aged rat brains in comparison with the young control group. More specifically, clusters of reduce…