Cajaninstilbene acid (CSA) exerts cytoprotective effects against oxidative stress through the Nrf2-dependent antioxidant pathway.
Cajaninstilbene acid (CSA), an active compound separated from pigeon pea leaves, possesses the highly efficient antioxidant activities. Transcription factor nuclear factor-erythroid 2-related factor 2 (Nrf2) is an important regulator of cellular oxidative stress. This study examined the role of Nrf2 in CSA-mediated antioxidant effects on human hepatocarcinoma (HepG2) cell line. The generation of reactive oxygen species (ROS) upon H2O2 and CSA treatment was lower than that of H2O2 alone. CSA activated Nrf2 as evaluated by Western blotting. A luciferase reporter assay also demonstrated that CSA-activated signaling resulted in the increased transcriptional activity of Nrf2 through binding to t…
UV-Induced Changes of Active Components and Antioxidant Activity in Postharvest Pigeon Pea [Cajanus cajan (L.) Millsp.] Leaves
In this study, the effect of UV irradiation (UV-A, UV-B, and UV-C) on phytochemicals, total phenolics, and antioxidant activity of postharvest pigeon pea leaves was evaluated. The response of pigeon pea leaves to UV irradiation was phytochemical specific. UV-B and UV-C induced higher levels of phytochemicals, total phenolics, and antioxidant activity in pigeon pea leaves compared with UV-A. Furthermore, UV-B irradiation proved to possess a long-lasting effect on the levels of phenolics and antioxidant activity. After adapting for 48 h at 4 °C following 4 h UV-B irradiation, total phenolics and antioxidant activity were approximately 1.5-fold and 2.2-fold increased from 39.4 mg GAE/g DM and …
Negative-pressure cavitation coupled with aqueous two-phase extraction and enrichment of flavonoids and stilbenes from the pigeon pea leaves and the evaluation of antioxidant activities
Abstract An efficient extraction method, negative-pressure cavitation coupled with aqueous two-phase extraction (NPC-ATPE), was developed to simultaneously extract and enrich flavonoids and stilbenes from pigeon pea leaves. The main bioactive compounds, cajaninstilbene acid (CSA), longistyline C (LLC), pinostrobin (PI) and pinostrobin chalcone (PIC) were chosen as target compounds. The extraction conditions were optimized using central composite design combined with response surface methodology. The optimized conditions were: concentration of (NH 4 ) 2 SO 4 21% (w/w), concentration of ethanol 23% (w/w) and temperature 30 °C, negative pressure −0.06 MPa, extraction time 30 min and the liquid…
Free radical scavenging capability, antioxidant activity and chemical constituents of Pyrola incarnata Fisch. leaves
Abstract In the present study, the crude extract and its four fractions of petroleum ether, ethyl acetate, n-butanol, water from Pyrola incarnata Fisch. leaves were prepared and their antioxidant activities were investigated by using four antioxidant assays in vitro. The ethyl acetate fraction showed remarkably strong antioxidant activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ABTS radical scavenging, reducing power and β-carotene-linoleic acid bleaching, which were almost comparable to capacities of the positive controls (VC and BHT). The ethyl acetate fraction had the highest total phenolic and total flavonoid contents. Furthermore, Pearson correlation analysis was …