6533b874fe1ef96bd12d6015

RESEARCH PRODUCT

Free radical scavenging capability, antioxidant activity and chemical constituents of Pyrola incarnata Fisch. leaves

Yu-jie FuYuangang ZuCheng-bo GuMeng LuoDong-yang ZhangThomas EfferthFan-song MuXiao-hui YaoChunying Li

subject

chemistry.chemical_classificationAntioxidantChromatographyABTSDPPHmedicine.medical_treatmentFlavonoidEthyl acetateHigh-performance liquid chromatographychemistry.chemical_compoundchemistrymedicinePetroleum etherAgronomy and Crop ScienceScavenging

description

Abstract In the present study, the crude extract and its four fractions of petroleum ether, ethyl acetate, n-butanol, water from Pyrola incarnata Fisch. leaves were prepared and their antioxidant activities were investigated by using four antioxidant assays in vitro. The ethyl acetate fraction showed remarkably strong antioxidant activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ABTS radical scavenging, reducing power and β-carotene-linoleic acid bleaching, which were almost comparable to capacities of the positive controls (VC and BHT). The ethyl acetate fraction had the highest total phenolic and total flavonoid contents. Furthermore, Pearson correlation analysis was used to analyze the correlation. According to the results, a significant correlation between total phenolic contents and antioxidant activities were observed. At last the ethyl acetate fraction was analyzed by HPLC. Eight kinds of phenolic compounds were identified by ESI-MSn.

yearjournalcountryeditionlanguage
2013-08-01Industrial Crops and Products
https://doi.org/10.1016/j.indcrop.2013.04.058