Effect of different cryosurgical protocols using liquid nitrogen on bone tissue: a histomorphological analyze

The aim of the present experimental study was to evaluate the morphological effects of different liquid nitrogen cryosurgery protocols on bone tissue. The femoral diaphyses of 42 Wistar rats were exposed to three local and sequential applications of liquid nitrogen for 1 or 2 min, intercalated with periods of 5 min of passive thawing. The animals were sacrificed after 1, 2, 4 and 12 weeks and the specimens obtained were processed and analyzed histomorphologically. Histologically, an increase in bone necrosis was observed for the two protocols in the second week after cryotherapy. A significant osteogenic phase was observed after 4 weeks. Moreover, complete remodeling process was encountered…