6533b7d2fe1ef96bd125f71a
RESEARCH PRODUCT
Effect of different cryosurgical protocols using liquid nitrogen on bone tissue: a histomorphological analyze
Fábio Wildson Gurgel CostaRosana Maria Andrade PessoaEduardo Costa Studart SoaresGerly Anne De Castro Britosubject
Pathologymedicine.medical_specialtyNecrosisChemistrymedicine.medical_treatmentObservation periodOdontologíaCryotherapyLiquid nitrogen:CIENCIAS MÉDICAS [UNESCO]Bone tissueCiencias de la saludCryosurgerymedicine.anatomical_structureUNESCO::CIENCIAS MÉDICASmedicinemedicine.symptomGeneral Dentistrydescription
The aim of the present experimental study was to evaluate the morphological effects of different liquid nitrogen cryosurgery protocols on bone tissue. The femoral diaphyses of 42 Wistar rats were exposed to three local and sequential applications of liquid nitrogen for 1 or 2 min, intercalated with periods of 5 min of passive thawing. The animals were sacrificed after 1, 2, 4 and 12 weeks and the specimens obtained were processed and analyzed histomorphologically. Histologically, an increase in bone necrosis was observed for the two protocols in the second week after cryotherapy. A significant osteogenic phase was observed after 4 weeks. Moreover, complete remodeling process was encountered at the end of the morphological observation period (12 weeks), especially on oneminute protocol. Thus, this study indicated that the 2-min protocol produced more marked bone necrosis than the 1-min protocol. In addition, the second experimental week was critical for bone necrosis with either cryotherapy protocol. Further studies are important for the understanding of the long-term behavior of bone tissue after cryoapplication of liquid nitrogen
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-01-01 | Journal of Clinical and Experimental Dentistry |