showing 4 related works from this author
Differential binding cell-SELEX method to identify cell-specific aptamers using high-throughput sequencing
2018
AbstractAptamers have in recent years emerged as a viable alternative to antibodies. High-throughput sequencing (HTS) has revolutionized aptamer research by increasing the number of reads from a few (using Sanger sequencing) to millions (using an HTS approach). Despite the availability and advantages of HTS compared to Sanger sequencing, there are only 50 aptamer HTS sequencing samples available on public databases. HTS data in aptamer research are primarily used to compare sequence enrichment between subsequent selection cycles. This approach does not take full advantage of HTS because the enrichment of sequences during selection can be due to inefficient negative selection when using live…
Injury-activated glial cells promote wound healing of the adult skin in mice
2018
Cutaneous wound healing is a complex process that aims to re-establish the original structure of the skin and its functions. Among other disorders, peripheral neuropathies are known to severely impair wound healing capabilities of the skin, revealing the importance of skin innervation for proper repair. Here, we report that peripheral glia are crucially involved in this process. Using a mouse model of wound healing, combined with in vivo fate mapping, we show that injury activates peripheral glia by promoting de-differentiation, cell-cycle re-entry and dissemination of the cells into the wound bed. Moreover, injury-activated glia upregulate the expression of many secreted factors previously…
Adult Stem Cell-Derived Extracellular Vesicles in Cancer Treatment: Opportunities and Challenges
2020
Adult stem cells (SCs) participate in tissue repair and homeostasis regulation. The relative ease of SC handling and their therapeutic effect has made of these cell popular candidates for cellular therapy. However, several problems interfere with their clinical application in cancer treatment, like safety issues, unpredictable pro-tumour effects, and tissue entrapment. Therefore cell-free therapies that exhibit SC properties are being investigated. It is now well known that adult SCs exhibit their therapeutic effect via paracrine mechanisms. In addition to secretory proteins, SCs also release extracellular vesicles (EV) that deliver their contents to the target cells. Cancer treatment is on…
Role of nerve - derived cells of neural crest origin in skin repair
2017
Perifērā neiropātija, var ietekmēt traumētas ādas atveseļošanas norisi. Izmantojot peļu brūču dzīšanas modeli, kurā glijas šūnas ir ģenētiski iezīmētas, mēs novērojām, ka ādas bojājums izraisa Švāna šūnu aktivāciju un migrāciju no nerva šķiedrām uz ievainotajiem audiem. Analizējot aktivētās glijas šūnas, mēs konstatējām, ka tās sekretē virkni faktoru, kam iepriekš novērota saistība ar brūču dzīšanu, un, aktivējot TGFβ signālceļu, tie veicina miofibroblastu veidošanos. Glijas šūnu skaita samazināšanās Sox10 inaktivētā gēna pelēs kavēja brūces aizvēršanos. Pretēji tam, glijas šūnu skaita pieaugums stimulēja miofibroblastu veidošanos. Tādējādi aktivētām glijas šūnām vai to izdalītiem faktoriem…